期刊
MEASUREMENT SCIENCE AND TECHNOLOGY
卷 21, 期 10, 页码 -出版社
IOP Publishing Ltd
DOI: 10.1088/0957-0233/21/10/102002
关键词
FLIM; FRET; fluorescence; anisotropy; GFP; biosensors
Fluorescence lifetime imaging microscopy (FLIM) and fluorescence anisotropy imaging microscopy (FAIM) are versatile tools for the investigation of the molecular environment of fluorophores in living cells. Owing to nanometre-scale interactions via Forster resonance energy transfer (FRET), FLIM and FAIM are powerful microscopy methods for the detection of conformational changes and protein-protein interactions reflecting the biochemical status of live cells. This review provides an overview of recent advances in photonics techniques, quantitative data analysis methods and applications in the life sciences.
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