期刊
APPLIED SURFACE SCIENCE
卷 328, 期 -, 页码 444-452出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.apsusc.2014.12.070
关键词
Electrospinning; Zein ultrafine fiber; Gold nanoparticles; Laccase; Biosensor
类别
资金
- National High-Tech R&D Program of China [2012AA030313]
- National Natural Science Foundation of China [51006046, 51203064, 21201083, 51163014]
- Changjiang Scholars and Innovative Research Team in University [IRT1135]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Industry-Academia-Research Joint Innovation Fund of Jiangsu Province [BY2012068]
- Science and Technology Support Program of Jiangsu Province [SBE201201094]
- Innovation Program for Graduate Education in Jiangsu Province [CXZZ13_07]
A novel laccase biosensor based on a new composite of laccase-gold nanoparticles (Au NPs)-crosslinked zein ultrafine fibers (CZUF) has been fabricated for catechol determination in real solution samples. Firstly, crosslinked zein ultrafine fibers containing gold nanoparticles (A-CZUF) were prepared by combining electrospinning and one-step reduction method using poly(ethyleneimine) (PEI) as reducing and crosslinking agent. A smooth morphology and relative average distribution of A-CZUF were depicted by scanning electron microscope (SEM) and transmission electron microscopy (TEM). The Fourier transform infrared spectroscopy (FT-IR) analysis indicated that PEI molecules attached to the surface of the zein ultrafine fibers via the reaction of functional groups between PEI and glyoxal. The results obtained from ultraviolet visible spectroscopy (UV-vis spectroscopy), X-ray diffraction (XRD) and thermal gravimetric analysis (TGA) for A-CZUF confirmed the existence of Au NPS coated on the surface of CZUF. Square wave voltammetry (SWV) and cyclic voltammetry (CV) were used to detect the electrochemical performance of the proposed biosensor. The results demonstrated that this biosensor possessed a high sensitive detection to catechol, which was attributed to the direct electron transfer (DET) facilitated by Au NPs and high catalytic ability obtained from laccase. In addition, the proposed biosensor exhibited good reproducibility, stability and selectivity. (C) 2014 Elsevier B.V. All rights reserved.
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