Review
Cell & Tissue Engineering
Wen-zhao Liu, Zhan-jun Ma, Jie-ru Li, Xue-wen Kang
Summary: SCI often leads to serious motor and sensory dysfunction of the limbs below the injured segment, causing physical, psychological harm and economic burden. MSC therapy for SCI has shown promise but faces challenges like low survival rate and immune rejection, while exosomes derived from MSCs have great potential in the treatment of SCI.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell & Tissue Engineering
Mayank Sharma, Michael A. Bellio, Merline Benny, Shathiyah Kulandavelu, Pingping Chen, Chawisa Janjindamai, Chenxu Han, Liming Chang, Shanique Sterling, Kevin Williams, Andreas Damianos, Sunil Batlahally, Kaitlyn Kelly, Daniela Aguilar-Caballero, Ronald Zambrano, Shaoyi Chen, Jian Huang, Shu Wu, Joshua M. Hare, Augusto Schmidt, Aisha Khan, Karen Young
Summary: This study compares the efficacy of GMP-grade EVs obtained from Wharton's Jelly MSCs (WJ-MSCs) and bone marrow (BM-MSCs), determines the optimal dosing and route of administration, and evaluates its long-term effects. WJ-MSC EVs have better effects on cardiac remodeling, while low, medium, and high dose WJ-MSC EVs have similar cardiopulmonary regenerative effects. Both IT and IV WJ-MSC EVs improve vascular density and reduce PH in hyperoxic rats. Long-term studies show that a single early MSC EV dose has pulmonary vascular protective effects 3 months after administration.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Review
Cell & Tissue Engineering
Davod Pashoutan Sarvar, Hosein Effatpanah, Parvin Akbarzadehlaleh, Karim Shamsasenjan
Summary: The study highlights the important role of MSC-derived extracellular vesicles (MSC-EVs) in hematopoietic regulation and immune response after transplantation. MSC-EVs directly fuse with hematopoietic stem cell membranes, affecting their fate, and regulate the proliferation and differentiation of hematopoietic stem cells through the release of various bioactive factors.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Li Hu, Jie Wang, Donghai Lin, Yueyao Shen, Huijie Huang, Yue Cao, Yan Li, Kai Li, Yanfang Yu, Youjia Yu, Chunyan Chu, Lianju Qin, Xiaojian Wang, Haifeng Zhang, David Fulton, Feng Chen
Summary: This study developed a high-yield mesenchymal stem cell-derived nanovesicles (MSC-NVs) and demonstrated their therapeutic effects in preclinical models of pulmonary hypertension (PH). MSC-NVs showed significant uptake in hypertensive lungs, pulmonary artery lesions, and pulmonary artery smooth muscle cells (PASMCs), and inhibited the proliferation, migration, and phenotype switching of PASMCs. miRNA-sequencing analysis revealed that MSC-NVs are rich in miR-1256-5p and miR-100-5p, which may account for their therapeutic effects in PH. Depletion of miR-1256-5p and miR-100-5p in MSCs diminished the protective effects of MSC-NVs.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Slaven Crnkovic, Francesco Valzano, Elisabeth Fliesser, Juergen Gindlhuber, Helene Thekkekara Puthenparampil, Maria Basil, Mike P. Morley, Jeremy Katzen, Elisabeth Gschwandtner, Walter Klepetko, Edward Cantu, Heimo Wolinski, Horst Olschewski, Joerg Lindenmann, You-Yang Zhao, Edward E. Morrisey, Leigh M. Marsh, Grazyna Kwapiszewska
Summary: This study utilized single-cell RNA sequencing to reveal cellular composition changes in pulmonary arterial hypertension and donor lungs. The results showed that remodeling altered communication between immune and structural cells, especially smooth muscle cells. Different populations of smooth muscle cells were enriched in specific biological processes and associated with the development of vascular remodeling.
Article
Biochemistry & Molecular Biology
Yu-Show Fu, Po-Ru Chen, Chang-Ching Yeh, Jian-Yu Pan, Wen-Chuan Kuo, Kuang-Wen Tseng
Summary: The study shows that xenografted human umbilical mesenchymal stem cells can survive in rat eye tissues for a long time and effectively support the structural integrity of injured corneal tissues, promoting repair and reducing inflammation.
Article
Biochemical Research Methods
Yoshihiko Shimazawa, Kosuke Kusamori, Mari Tsujimura, Asuka Shimomura, Ryo Takasaki, Yukiya Takayama, Kazunori Shimizu, Satoshi Konishi, Makiya Nishikawa
Summary: This study demonstrated that forming multicellular spheroids of MSCs improved their pulmonary delivery and survival after intravenous injection, as well as their therapeutic potential against inflammatory pulmonary diseases.
BIOTECHNOLOGY JOURNAL
(2022)
Review
Cell Biology
Shengnan Yang, Peipei Liu, Yale Jiang, Zai Wang, Huaping Dai, Chen Wang
Summary: Idiopathic pulmonary fibrosis (IPF) is a progressive lung disease with unknown causes, and currently the only medications that can slow down the progression of the disease and improve survival rate are pirfenidone and nintedanib. The use of mesenchymal stem cells (MSCs) provides a new hope for treating interstitial lung disease, but optimal treatment protocols are still lacking.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Babak Jahangiri, Mohammad Khalaj-Kondori, Elahe Asadollahi, Leyli Purrafee Dizaj, Majid Sadeghizadeh
Summary: This study found that exosomes derived from mesenchymal stem cells (MSC-Exos) can suppress proliferation, migration, invasion, and metastasis while inducing apoptosis of colorectal cancer (CRC) cells through the miR-100/mTOR/miR-143 axis. These findings suggest that MSC-Exo treatment and miR-100 restoration may be potential therapeutic strategies for CRC.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Review
Biochemistry & Molecular Biology
Serena Barachini, Letizia Biso, Shivakumar Kolachalam, Lacopo Petrini, Roberto Maggio, Marco Scarselli, Biancamaria Longoni
Summary: Pancreatic islet transplantation is a treatment for Type 1 diabetic patients, and mesenchymal stem cells (MSCs) have shown potential in controlling graft rejection. MSCs can interact with immune system cells through direct contact or their secretome. This review discusses the role of MSCs in controlling the immune system and promoting repair in pancreatic islet transplantation, as well as challenges such as oxidative stress and impaired vasculogenesis. Preconditioning MSCs with hypoxia exposure or using stem cells with angiogenic potential in organoids may help improve transplant outcomes.
Article
Chemistry, Multidisciplinary
Ji Sun, Jingbo Li, Zhikun Huan, Stephen J. Pandol, Dechen Liu, Luoran Shang, Ling Li
Summary: Type 1 diabetes mellitus is characterized by autoimmune damage of pancreatic beta cells. This study develops mesenchymal stem cell (MSC)-laden composite beta cell porous microgels (MGs) to address sustainability for the treatment of diabetes. The results demonstrate that the MGs have great potential for the treatment of diabetes providing a promising platform for clinical beta cell transplantation.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Engineering, Biomedical
Maosheng Cao, Yun Zhao, Tong Chen, Zijiao Zhao, Boqi Zhang, Chenfeng Yuan, Xin Wang, Lu Chen, Nan Wang, Chunjin Li, Xu Zhou
Summary: This study found that AMSC-EXOs can protect against metabolic disturbances, improve ovarian polycystic and enhance fertility. The study also discovered that AMSC-EXOs transfer miRNA to the liver, thereby improving metabolic dysfunction and reproductive ability in PCOS. This provides a theoretical basis for using rat adipose stem cells and their secreted exosomes to treat PCOS.
Article
Medicine, Research & Experimental
Saeed Oraee-Yazdani, Roozbeh Tavanaei, Fatemeh Rostami, Atieh Hajarizadeh, Marzieh Mehrabadi, Mohammadhosein Akhlaghpasand, Mona Tamaddon, Samin Khannejad, Kaveh Oraii Yazdani, Alireza Zali
Summary: This study examined the safety of suicide gene therapy using allogeneic adipose tissue-derived mesenchymal stem cells (ADSCs) carrying herpes simplex virus-thymidine kinase (HSV-TK) gene in patients with recurrent glioblastoma multiforme (GBM). The results showed that this gene therapy protocol was safe and well tolerated, and there were improvements in overall survival and progression-free survival. Further clinical trials are needed to validate these findings and investigate the efficacy of this protocol.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Medicine, Research & Experimental
Hyun Jik Lee, Chang Woo Chae, Ho Jae Han
Summary: Mesenchymal stem cell (MSC) transplantation has potential for treating diabetes mellitus (DM) and its complications. However, hyperglycemia can cause senescence and dysfunction in transplanted and resident MSCs due to mitochondrial dysfunction. Improving mitochondrial function could enhance the therapeutic efficacy of MSC transplantation in DM.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Review
Biochemistry & Molecular Biology
Bernard J. Smilde, Esmee Botman, Teun J. de Vries, Ralph de Vries, Dimitra Micha, Ton Schoenmaker, Jeroen J. W. M. Janssen, Elisabeth M. W. Eekhoff
Summary: Fibroblasts play a crucial role in maintaining the extracellular matrix of connective tissues. Recent studies have found donor-derived fibroblasts in bone marrow transplant recipients, though some findings were not replicated. This systematic review provides an overview of hematopoietic stem cells differentiating into fibroblasts in various tissues, revealing evidence of fibroblasts derived from hematopoietic stem cells in almost all organs.
