期刊
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
卷 44, 期 -, 页码 430-439出版社
ELSEVIER
DOI: 10.1016/j.msec.2014.07.049
关键词
Co-delivery; Gene; Drug; PAMAM
资金
- Fund of the Focused Project of Science and Technology of Zhanjiang [2012C3106015]
- Doctoral Research Program of Guangdong Medical College [XB1303]
- National Natural Science Foundation of China [21074152, 51273216]
Cationic micellar nanoparticles for chemotherapeutic drugs and therapeutic gene co-delivery were prepared based on a poly-(N-epsilon-carbobenzyloxy-L-lysine) (PZLL) and dendritic polyamidoamine (PAMAM) block copolymer (PZLL-D3). PZLL-D3 was synthesized by a copper-catalyzed azide alkyne cyclization (click) reaction between alpha-alkyne-PZLL and azide focal point PAMAM dendrons. Its structure was characterized by H-1 NMR and FTIR, and its buffering capability was determined by acid-base titration. MTT, agarose gel electrophoresis and flow cytometry studies showed that PZLL-D3 revealed low in vitro cytotoxicity, strong pDNA condensation ability, protection of pDNA against deoxyribonuclease I degradation and high gene transfection efficiency in 293T and HeLa cells. In addition, the micellar nanoparticles delivered pDNA and anticancer drug doxorubicin (DOX) simultaneously and efficiently to tumor cells, and the DOX loaded nanoparticles showed sustained in vitro release at pH = 7.4 and 5.8. (C) 2014 Published by Elsevier B.V.
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