Article
Chemistry, Multidisciplinary
Ziyang Sun, Haoran Liu, Dandan Dai, Hao Lyu, Ruochuan Huang, Wenzhao Wang, Chengchen Guo
Summary: This study presents a promising injectable cell-laden hydrogel system based on silk acid, which exhibits good cytocompatibility and injectability. The hydrogel has potential applications in 3D cell culture, targeted cell delivery, and tissue engineering.
CHEMICAL COMMUNICATIONS
(2023)
Article
Materials Science, Multidisciplinary
Shuying Li, Chuanzhen Huang, Hanlian Liu, Xu Han, Zhichao Wang, Jun Huang, Yonggan Yan, Zhen Wang
Summary: Cell adhesion is crucial for tissue formation. Developing a bioink with tunable mechanical properties and enhanced cell adhesion is a challenge in tissue engineering. In this study, a printable hydrogel was developed by modifying GelMA/HAMA with SilMA. The hydrogel showed good photocuring behavior and was suitable for DLP 3D bioprinting. SilMA content could tailor the mechanical and biological properties of the hydrogel. SilMA/GelMA/HAMA hydrogel exhibited higher adhesion strength and enhanced cell actin expression. The 3D printed scaffold structure remained stable and supported cell survival and proliferation. The hydrogel has broad implications for customized 3D structures in tissue engineering.
ACS APPLIED POLYMER MATERIALS
(2022)
Review
Biochemistry & Molecular Biology
Linlin Huang, Jifeng Shi, Wei Zhou, Qing Zhang
Summary: Silk fibroin (SF) has been widely studied and applied in various fields due to its excellent biocompatibility, machinability, and chemical modifiability. The process of obtaining regenerated silk fibroin (RSF) includes degumming, dissolving, dialysis, and centrifugation. Different dissolution methods of SF have significant impacts on the structure and performance of RSF, which are important for its subsequent processing and application.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Megan K. DeBari, Xiaodan Niu, Jacqueline V. Scott, Mallory D. Griffin, Sean R. Pereira, Keith E. Cook, Bin He, Rosalyn D. Abbott
Summary: The study reveals that therapeutic ultrasound can noninvasively trigger enhanced degradation of silk fibroin scaffolds by removing microbubbles on the scaffold surface. This ultrasound-responsive biomaterial is proven safe for human cells and effectively triggers scaffold degradation through human skin, increasing clinical relevance. The degradation profile can be adjusted noninvasively to improve regenerative outcomes.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Fengming Luan, Wangbei Cao, Chunhui Cao, Baizhou Li, Xiaoyu Shi, Changyou Gao
Summary: In this study, a new composite biological mesh named SFP was prepared and its mechanism and clinical application value were explored. The results showed that the SFP mesh exhibited good biocompatibility and biofunction in vitro and in vivo, promoting the growth of peritoneal mesenchymal cells and reducing the extent and scope of adhesion between the mesh and abdominal organs.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Michael Woeltje, Kristin L. Isenberg, Chokri Cherif, Dilbar Aibibu
Summary: A wet spinning process for regenerated silk fibroin fibers with only 4% protein content has been developed, achieving similar rheological behavior to native silk fibroin. The tensile strength of these wet-spun fibers is higher than that of all previous continuous spinning methods for regenerated and native silk fibroin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ying Shen, Xinyu Wang, Binbin Li, Yajin Guo, Kuo Dong
Summary: This study incorporated nanoparticles into a scaffold to improve hemostatic properties and stimulate cell adhesion. The scaffold loaded with nanoparticles exhibited higher blood clotting index and promoted platelets adhesion. In addition, the scaffold loaded with nanoparticles showed stimulation effects on cell proliferation, migration, and adhesion. Therefore, the scaffold loaded with nanoparticles has great potential as a wound dressing.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Aida Naghilou, Karolina Peter, Flavia Millesi, Sarah Stadlmayr, Sonja Wolf, Anda Rad, Lorenz Semmler, Paul Supper, Leon Ploszczanski, Jiliang Liu, Manfred Burghammer, Christian Riekel, Alexander Bismarck, Ellen H. G. Backus, Helga Lichtenegger, Christine Radtke
Summary: The dragline silk of Trichonephila spiders has shown potential for nerve regeneration. This study investigated the effects of sterilization methods on the silk's properties and examined the migration and proliferation of rat Schwann cells on the treated silk fibers. The results suggest that ethanol treatment promotes faster cell migration and highlight the importance of silk's stiffness and composition in supporting nerve growth.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Polymer Science
Mahdi Yonesi, Milagros Ramos, Carmen Ramirez-Castillejo, Rocio Fernandez-Serra, Fivos Panetsos, Adrian Belarra, Margarita Chevalier, Francisco J. Rojo, Jose Perez-Rigueiro, Gustavo Guinea, Daniel Gonzalez-Nieto
Summary: Central nervous system diseases impose a significant social and economic burden, with inflammation being a common factor that affects the stability of biomaterials and therapies. This study explored the stability of silk fibroin hydrogels in different neuroinflammatory contexts using in vitro and in vivo models. The results showed that silk hydrogels remained relatively stable and did not degrade extensively in the presence of inflammation, contrasting with the rapid degradation of collagen under the same conditions. These findings support the potential use of silk fibroin hydrogels for intracerebral applications in cerebral pathologies.
Article
Chemistry, Physical
Zuobing Xiao, Huiqin Liu, Qixuan Zhao, Yunwei Niu, Di Zhao
Summary: In this study, spherical structured nanocapsules (SSPM) were successfully prepared by mixing silk fibroin (SF) and dopamine (DA) in an aqueous solution and preparing SiO2 nanoparticles. The nanocapsules showed good washing durability and biocompatibility, making them suitable for applications in functional textiles and medical textiles. The use of double dynamic bonds provided a simple and green strategy for the preparation of nanocomposites.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Polymer Science
Peng Pan, Cheng Hu, Ahui Liang, Xueping Liu, Mengqi Fang, Shanlong Yang, Yadong Zhang, Mingzhong Li
Summary: The study developed a hybrid scaffold with antibacterial and healing-guiding functions. Results showed that the scaffold exhibited significant antibacterial activity against bacteria when the PHMB/SF ratio was greater than 1/100, and promoted cell adhesion, spreading, and proliferation when the ratio was less than 2/100.
Article
Chemistry, Physical
Zhenguo Wang, Yu Zhang, Pingping Tang, Zhiming Deng, Ping He, Meng-Jie Chen, Zhong-Zhen Yu, Hao-Bin Zhang
Summary: In this study, highly conductive and strong graphene fibers were fabricated by controlling the rheology and spinnability of silk fibroin (SF) solution. The amphiphilic SF formed hydrogen bonds and 7C-7C interactions with graphene oxide (GO) sheets, enhancing the gel strength of spinning solution and enabling conformation adjustment and sheet alignment of GO/SF fibers under large drawing ratio. After chemical reduction, the fibers exhibited exceptional tensile strength and high electrical conductivity, making them suitable for versatile applications in multifunctional fabrics, energy conversion, and health protection.
Article
Materials Science, Biomaterials
Kolton C. Sandau, Elizabeth M. Arrigali, Bogdan A. Serban, Monica A. Serban
Summary: This study investigates the colorimetric sensing properties of Bombyx mori silk fibroin (SF) and its applications in pH sensing and ultraviolet light exposure. Results show that reconstituted SF can undergo visible color changes in response to pH changes and ultraviolet light exposure, with the intensity of the color corresponding to the intensity of the stimuli. The color changes were reversible and interchangeable, with different colors observed under different pH conditions. The sensing capabilities of SF can be enhanced by incorporating additional tryptophan into the protein.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Julie F. Jameson, Marisa O. Pacheco, Jason E. Butler, Whitney L. Stoppel
Summary: Sponge-like biomaterials made from silk fibroin show promise as degradable materials in clinical applications due to their controllable breakdown and tunable parameters. In vitro degradation rates of silk sponges were measured, and the study investigated the mechanism and rate constants for biomaterial degradation. Experimental results suggest that modified first order reaction kinetics may predict the degradation rates of lyophilized silk sponges, providing a tool for future mathematical representations of silk biomaterial degradation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Polymer Science
Hafez Jafari, Arezoo Dadashzadeh, Saeid Moghassemi, Payam Zahedi, Christiani A. Amorim, Amin Shavandi
Summary: A new Cs-SF hydrogel has been developed in this study, which allows for spatially homogeneous distribution of ovarian cells and demonstrates 167% proliferation after 7 days. This hydrogel combines the toughness and flexibility of SF, and the phenolic chemistry shows potential for providing microstructures for encapsulating human ovarian stromal cells.
Article
Cell & Tissue Engineering
Matthew Cheng, Jan Janzekovic, Mina Mohseni, Flavia Medeiros Savi, Jacqui McGovern, Graham Galloway, Clement Wong, Siamak Saifzadeh, Michael Wagels, Dietmar W. Hutmacher
Summary: Scaffold-guided breast tissue engineering has the potential to revolutionize reconstructive breast surgery. This study introduces an original large animal model to investigate scaffold-guided breast tissue engineering concepts in a controlled and reproducible manner. The model demonstrated flexibility in studying multiple treatment conditions per animal and has been validated through a long-term 12-month experiment, surpassing shorter published studies.
TISSUE ENGINEERING PART C-METHODS
(2021)
Article
Biochemistry & Molecular Biology
Margaux Vigata, Christoph Meinert, Nathalie Bock, Bronwin L. Dargaville, Dietmar W. Hutmacher
Summary: Water in hydrogels plays a significant role in biocompatibility and hemocompatibility. The ionic strength and pH affect the mesh size of hydrogels, potentially impacting drug delivery applications, while drug loading increases non-freezable bound water. This research provides insights into molecular arrangements at the hydrogel-polymer interface and their response to environmental cues.
Letter
Ophthalmology
Luisa H. Colorado, Samantha J. Dando, Damien G. Harkin, Katie Edwards
CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2021)
Article
Cell & Tissue Engineering
Olivia J. McGifford, Damien G. Harkin, Leila Cuttle
Summary: ROCK inhibitors can impact cell behavior and have potential for optimizing tissue engineering applications. However, the current understanding of their effects on epidermal keratinocytes is unclear, highlighting the need for further research.
TISSUE ENGINEERING PART B-REVIEWS
(2022)
Article
Surgery
George Castrisos, Isabel Gonzalez Matheus, David Sparks, Martin Lowe, Nicola Ward, Marjoree Sehu, Marie-Luise Wille, Yun Phua, Flavia Medeiros Savi, Dietmar Hutmacher, Michael Wagels
Summary: This study presents a novel bone replacement technique based on regenerative matching axial vascularisation (RMAV) and its clinical applications. The technique utilizes 3D-printed medical-grade polycaprolactone and tricalcium phosphate (mPCL-TCP) scaffolds wrapped in vascularised free corticoperiosteal flaps for bone defect reconstruction, achieving successful outcomes.
JOURNAL OF PLASTIC RECONSTRUCTIVE AND AESTHETIC SURGERY
(2022)
Article
Cell & Tissue Engineering
David S. Sparks, Flavia Medeiros Savi, Siamak Saifzadeh, Marie-Luise Wille, Michael Wagels, Dietmar W. Hutmacher
Summary: This study presents a novel approach for bone healing in critical size bone defects using a corticoperiosteal flap. The method has been proven effective in a large animal model and demonstrates potential for clinical application.
TISSUE ENGINEERING PART C-METHODS
(2022)
Article
Engineering, Biomedical
Markus Laubach, Philipp Kobbe, Dietmar W. Hutmacher
Summary: Lumbar fusion is a common treatment for spinal diseases, but current cage materials have limitations. Recently, biodegradable cages and scaffold-guided bone regeneration have shown potential for better outcomes. Surface modification of implants can also enhance tissue integration and reduce infections. Further research is needed to improve these techniques.
Review
Multidisciplinary Sciences
B. L. Dargaville, D. W. Hutmacher
Summary: Water plays a crucial role in controlling physical, chemical, and biological processes, particularly in materials such as hydrogels. However, there is still much to be understood about how water interacts with these materials and its impact on biological processes. This perspective outlines the importance of water in biomaterials science and provides directions for future research.
NATURE COMMUNICATIONS
(2022)
Article
Health Care Sciences & Services
Isabel Gonzalez Matheus, Dietmar W. Hutmacher, Sarah Olson, Michael Redmond, Allison Sutherland, Michael Wagels
Summary: This study aims to investigate the reconstructive capacity of a hybridized technique for large skull defects. The technique combines autologous and synthetic materials to promote bone regeneration through a biodegradable 3D-printed scaffold wrapped in vascularized autotransplanted periosteum. The results of the study are expected to be available before the end of 2023.
JMIR RESEARCH PROTOCOLS
(2022)
Article
Engineering, Biomedical
Agathe Bessot, Jennifer Gunter, David Waugh, Judith A. Clements, Dietmar W. Hutmacher, Jacqui McGovern, Nathalie Bock
Summary: Increasing evidence highlights the important role of bone marrow adipocytes in prostate cancer bone metastases. However, lack of relevant models has hindered the understanding of human BM-adipocyte effects in this microenvironment. This study combines tunable GelMA-based hydrogels with biomimetic culture of human cells to engineer human bone tumor microenvironments containing BM-adipocytes. In vitro culture of human osteoprogenitors, adipocytes, and PCa cells in GelMA hydrogels leads to the formation of mineralized, adipose, and PCa tumor 3D microtissues, respectively. The stiffness of hydrogels regulates osteoblast mineralization and tumor spheroid formation, while coculture with tumor cells induces morphological changes and delipidation in BM-adipocytes. The in vivo model successfully forms a humanized fatty bone microenvironment and demonstrates that adipocytes support early bone tumor growth. This advanced platform combines natural aspects of the microenvironment with tunable properties for bone tumor research.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Pharmacology & Pharmacy
Tara Shabab, Onur Bas, Bronwin L. Dargaville, Akhilandeshwari Ravichandran, Phong A. Tran, Dietmar W. Hutmacher
Summary: This study combines melt-extrusion based 3D printing and porogen leaching to develop tunable multiphasic scaffolds for dental tissue regeneration. The scaffolds are highly adjustable in terms of mechanical properties, degradation kinetics, and surface morphology. The research also demonstrates improved cell attachment, proliferation, and extracellular matrix production on these multiscale scaffolds compared to single-scale counterparts, indicating their potential for enhanced tissue regeneration. Furthermore, the study explores the use of these scaffolds as drug delivery devices and shows that sustained drug release can be achieved. These findings strongly support further development of these scaffolds for dental tissue regeneration applications.
Article
Engineering, Manufacturing
Buddhi Herath, Markus Laubach, Sinduja Suresh, Beat Schmutz, J. Paige Little, Prasad K. D. V. Yarlagadda, Dietmar W. Hutmacher, Marie-Luise Wille
Summary: A modular design workflow was developed to facilitate the efficient design and manufacture of patient-specific scaffolds for scaffold-guided bone regeneration (SGBR). The workflow allows for semi-automatic filling of defect cavities, patient specificity, ideal surgical scaffold insertion, and generation of scaffolds based on various pore architectures. The real-time feedback from surgeons shortens the time required to finalize a patient-specific scaffold design.
VIRTUAL AND PHYSICAL PROTOTYPING
(2023)
Review
Engineering, Biomedical
Markus Laubach, Frank Hildebrand, Sinduja Suresh, Michael Wagels, Philipp Kobbe, Fabian Gilbert, Ulrich Kneser, Boris M. Holzapfel, Dietmar W. Hutmacher
Summary: The treatment of bone defects is a challenging clinical problem with high reintervention rates and significant healthcare costs. Three-dimensional printing has revolutionized the treatment of bone defects by enabling personalized therapies with highly porous 3D-printed implants. However, there are still challenges to be addressed, including the development of guidelines, clinical trial design, and financing support.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Mairim Russo Serafini, Alexandra Mowat, Susanah Mustafa, Siamak Saifzadeh, Tara Shabab, Onur Bas, Nicholas O'Rourke, Dietmar W. Hutmacher, Flavia Medeiros Savi
Summary: This study evaluated the use of mPCL scaffolds loaded with PRP for hernia repair in sheep models, and found that the mPCL scaffolds enhanced soft tissue regeneration. After 6 months, the mPCL scaffolds showed similar mechanical properties, collagen, and vascular ingrowth compared to PP meshes. This pilot study supports the use of mPCL as a safe and effective biodegradable scaffold for hernia and vaginal prolapse repair.
BIOENGINEERING-BASEL
(2023)
Article
Orthopedics
Markus Laubach, Sinduja Suresh, Buddhi Herath, Marie-Luise Wille, Heide Delbrueck, Hatem Alabdulrahman, Dietmar W. Hutmacher, Frank Hildebrand
Summary: This study reports the successful application of patient-specific SGBR in the treatment of large complex long bone defects, highlighting the importance of scaffold design in promoting bone regeneration.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2022)
Article
Materials Science, Biomaterials
Mohammadreza Kasravi, Alireza Yaghoobi, Tahereh Tayebi, Mahsa Hojabri, Abdolkarim Talebi Taheri, Fatemeh Shirzad, Bahram Jambar Nooshin, Radman Mazloomnejad, Armin Ahmadi, Fatemeh A. Tehrani, Ghasem Yazdanpanah, Mohammad Hadi Farjoo, Hassan Niknejad
Summary: As a promising approach in translational medicine, the decellularization of discarded livers to produce bioscaffolds that support recellularization has potential in overcoming the limitations of conventional liver transplantation. In this study, the researchers investigated the use of matrix metalloproteinase (MMP) inhibition to preserve the extracellular matrix (ECM) during liver decellularization. The results demonstrated that the application of an MMP inhibitor significantly improved the preservation of ECM components and mechanical properties of the bioscaffolds, which supported cell viability and function in vitro. The study also confirmed that the MMP inhibition led to the inhibition of MMP2 and MMP9, providing a novel method to enhance ECM preservation during liver decellularization.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Mohammadsadegh Nadimifar, Weiguang Jin, Clara Coll-Satue, Gizem Bor, Paul Joseph Kempen, Ali Akbar Moosavi-Movahedi, Leticia Hosta-Rigau
Summary: This study presents a metal-phenolic self-assembly approach that can prepare nanoparticles fully made of hemoglobin. The nanoparticles exhibit good oxygen binding and releasing capabilities.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jyoti Kumari, Roel Hammink, Jochem Baaij, Frank A. D. T. G. Wagener, Paul H. J. Kouwer
Summary: Fibrosis is the formation of fibrous connective tissue in response to injury, leading to organ dysfunction. A novel hybrid hydrogel combining synthetic polyisocyanide with hyaluronic acid has been developed, showing strong antifibrotic properties.
BIOMATERIALS ADVANCES
(2024)
Letter
Materials Science, Biomaterials
Melissa Machado Rodrigues, Cristian Padilha Fontoura, Charlene Silvestrin Celi Garcia, Sandro Tomaz Martins, Joao Antonio Pegas Henriques, Carlos Alejandro Figueroa, Mariana Roesch Ely, Cesar Aguzzoli
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jessica Polak, David Sachs, Nino Scherrer, Adrian Suess, Huan Liu, Mitchell Levesque, Sabine Werner, Edoardo Mazza, Gaetana Restivo, Mirko Meboldt, Costanza Giampietro
Summary: Human skin equivalents (HSEs) play a crucial role in tissue engineering. This study introduces a 3D-printed culture insert to apply a static radial constraint on HSEs and examines its effects on tissue characteristics. The results show that the diameter of the culture insert significantly influences tissue contraction, fibroblast and matrix organization, keratinocyte differentiation, epidermal stratification, and basement membrane formation. This study provides important insights for the design of skin tissue engineering.
BIOMATERIALS ADVANCES
(2024)
Review
Materials Science, Biomaterials
Shiliang Chen, Tianming Du, Hanbing Zhang, Jing Qi, Yanping Zhang, Yongliang Mu, Aike Qiao
Summary: This paper reviewed the primary methods for improving the overall properties of biodegradable zinc stents. It discussed the mechanical properties, degradation behavior, and biocompatibility of various improvement strategies. Alloying was found to be the most common, simple, and effective method for improving mechanical properties. Deformation processing and surface modification further improved the mechanical properties and biological activity of zinc alloys. Meanwhile, structural design could endow stents with special properties. Manufacturing zinc alloys with excellent properties and exploring their interaction mechanism with the human body are areas for future research.
BIOMATERIALS ADVANCES
(2024)
