Review
Chemistry, Physical
Sylwia Grabska-Zielinska, Alina Sionkowska
Summary: This review provides an overview of the recent advances in silk fibroin (SF) biopolymer and its blends with other biopolymers as new biomaterials, highlighting the improved physico-chemical properties and potential applications in the biomedical field. The review also discusses the structure, forms, preparation methods, and some physico-chemical properties of silk fibroin-based mixtures, showcasing examples of binary and ternary mixtures with the addition of various components. Additionally, the advantages and disadvantages of different cross-linking methods are also presented.
Review
Biochemistry & Molecular Biology
Shahid Ud Din Wani, Mohammed Iqbal Zargar, Mubashir Hussain Masoodi, Sultan Alshehri, Prawez Alam, Mohammed M. Ghoneim, Areej Alshlowi, H. G. Shivakumar, Mohammad Ali, Faiyaz Shakeel
Summary: Silk fibroin, derived from silkworm cocoons, is widely used in biomedicine due to its superior biocompatibility and controllable degradation. It is employed in drug delivery systems to extend drug shelf life, control release, and provide new possibilities for treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Polymer Science
Traian V. Chirila
Summary: Fibroin, extracted from silk cocoons, has been widely used in biomedical applications, but its efficacy as a material for contact lenses remains limited and controversial, with no evidence of commercialization.
Article
Biotechnology & Applied Microbiology
Giorgio Rizzo, Marco Lo Presti, Cinzia Giannini, Teresa Sibillano, Antonella Milella, Giulia Guidetti, Roberta Musio, Fiorenzo G. Omenetto, Gianluca M. Farinola
Summary: Silk Fibroin (SF) obtained from Bombyx mori can be processed into scaffolds from aqueous solutions using chaotropic salts. By modifying the standard Ajisawa's method with CeCl3, it is possible to regenerate SF as a fibrous material with an ordered structure and doped with Ce+3 ions. Lanthanide ions are found to solubilize SF and favor the reprecipitation of fibrous SF, making them potential candidates for developing new photonic systems.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Polymer Science
Anna Tuwalska, Sylwia Grabska-Zielinska, Alina Sionkowska
Summary: This review summarizes recent advances in the field of chitosan and silk fibroin blends as new biomaterials. These blends have attracted attention from both academia and industry, but have not been summarized in a review paper yet.
Review
Biochemistry & Molecular Biology
Huihui Lu, Jiale Wang, Jun Li, Bingbing Gao, Bingfang He
Summary: Microneedles made of silk fibroin show great potential as a transdermal drug delivery system due to their minimal invasiveness, painlessness, and on-demand drug release capabilities. This review summarizes the recent research progress of silk fibroin microneedles, including their materials, processing technology, detection, drug release methods, and applications. Furthermore, the multidimensional research and development of silk fibroin are analyzed, and it is expected that silk fibroin microneedles will have excellent development prospects in various fields.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Materials Science, Multidisciplinary
L. Mogas-Soldevila, G. Matzeu, M. Lo Presti, F. G. Omenetto
Summary: Biomaterial composites based on reformulated silk protein offer new routes for environmentally friendly leather-like materials by controlling silk protein conformations, introducing other materials and natural additives, and utilizing computerized fabrication. These materials are strong, soft, pliable, durable, and biodegradable, with the ability to embed active molecules for sensing the surrounding environment.
MATERIALS & DESIGN
(2021)
Article
Biochemistry & Molecular Biology
Hai-Yan Wang, Yu-Qing Zhang, Zheng-Guo Wei
Summary: This study compared undegraded SF and degraded SF, finding differences in viscosity, surface tension, and elongation between the two. SEM analysis revealed both uSF and dSF existed in aqueous solution as micelles with a diameter of approximately 30 nm. In terms of catalytic activity and cell culture, degraded SF showed better performance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Nanoscience & Nanotechnology
Alessandra Maria Bossi, Alessio Bucciarelli, Devid Maniglio
Summary: Nanosized biomimetics prepared by molecular imprinting are showing potential as plastic antibodies in medicine for cell imaging and targeted therapies. Utilizing natural polymer silk fibroin to synthesize biocompatible MIP SF-NPs demonstrates high affinity recognition sites and biocompatibility.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Engineering, Biomedical
Yeshi Liang, Aleksandr Mitriashkin, Ting Ting Lim, James Cho-Hong Goh
Summary: The study demonstrates the significant potential of electrospun mats incorporating conductive PPy-encapsulated SF for cardiac tissue engineering, with good mechanical properties and electrical conductivity. Different types of cardiomyocytes exhibit varying contraction characteristics on the hybrid mats, while external stimulation can enhance contraction synchrony.
Review
Biochemistry & Molecular Biology
Minghui Li, Jiaqian You, Qiuyue Qin, Manxuan Liu, Yixin Yang, Kewen Jia, Yidi Zhang, Yanmin Zhou
Summary: Bone tissue engineering (BTE) utilizes silk fibroin (SF) to regulate bone regeneration and stimulate bone formation. SF is a promising material due to its tunable mechanical properties, biodegradability, and biocompatibility. This review introduces the structure and characteristics of SF, summarizes its immunological mechanism for osteogenesis, and emphasizes the various forms of SF biomaterials and their development prospects in BTE. Biomaterials based on SF have great potential in bone tissue engineering, and this review guides future design and research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Engineering, Biomedical
Prerak Gupta, Biman B. Mandal
Summary: Vascular tissue engineering is focusing on finding biologically active substitutes with in situ growth potential, with silk biomaterials showing remarkable potential due to their favorable biological and mechanical properties.
ACTA BIOMATERIALIA
(2021)
Review
Biotechnology & Applied Microbiology
Hai-Yan Wang, Yu-Qing Zhang, Zheng-Guo Wei
Summary: Silk fibroin from silkworm Bombyx mori has been extensively researched and applied in various fields in recent decades, including cosmetics, biomedicine, and biomaterials. Dissolution and regeneration of SF fibers is crucial for the application of silk protein-based materials, and the process directly impacts the characteristics and mechanical properties of resulting silk biomaterials. This review summarizes common solvents and dissolution methods for silk protein, properties of resulting SF protein, suitable uses of SF dissolved in corresponding solvents, and recent applications of SF in various biomaterials.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2021)
Review
Medicine, Research & Experimental
Ziyang Zhou, Jin Cui, Shunli Wu, Zhen Geng, Jiacan Su
Summary: Osteoarthritis is a common joint disease that causes physical and psychological harm to patients and burdens the healthcare system. Tissue engineering has been focusing on cartilage repair, with silk fibroin as a promising biomaterial due to its unique properties and capabilities for functional modification.
Review
Chemistry, Physical
Federica Paladini, Mauro Pollini
Summary: Bone tissue engineering is a multidisciplinary research field that aims to create biological substitutes for bone regeneration. The selection of appropriate biomaterials and structures remains a challenge, and silk fibroin is considered a valuable option due to its unique biological properties and processability.
Article
Engineering, Chemical
Luciana de Souza Guedes, Cesar Costapinto Santana, Douglas Neil Rutledge, Licarion Pinto, Isabel Cristina Sales Fontes Jardim, Lucilia Vilela de Melo, Marisa Masumi Beppu, Marcia Cristina Breitkreitz
Summary: The study aimed to investigate the recovery of minor constituents in palm oil using ultra-high-performance supercritical fluid chromatography. Despite ineffective clean-up procedures, a chemometric method successfully quantified lycopene, beta-carotene, and coenzyme Q10.
CANADIAN JOURNAL OF CHEMICAL ENGINEERING
(2021)
Article
Biophysics
Thiago B. Taketa, Rogerio A. Bataglioli, Joao Batista M. Rocha Neto, Marisa M. Beppu
Summary: The study revealed that polysaccharide-based polyelectrolyte multilayer films loaded with silver ions have a uniform polymer composition and antimicrobial activity, providing insights for engineering advanced materials with controlled spatio-temporal behavior.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Biochemistry & Molecular Biology
J. B. M. Rocha Neto, G. G. Lima, A. Fiamingo, L. G. L. Germiniani, T. B. Taketa, R. A. Bataglioli, G. A. T. da Silveira, J. V. L. da Silva, S. P. Campana-Filho, O. N. Oliveira Jr, M. M. Beppu
Summary: By adjusting the degree of deacetylation of chitosans and the pH of the solution, the properties of HA/CHI LbL films can be controlled, providing tailored film materials for various applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Biochemistry & Molecular Biology
Murilo Santos Pacheco, Douglas Barbieri, Classius Ferreira da Silva, Mariana Agostini de Moraes
Summary: Orally disintegrating films (ODFs) are a novel drug administration method that quickly releases drugs and allows absorption through the oral mucosa. Natural polymers can be used in ODFs to enhance their properties, either alone or blended with other synthetic or semi-synthetic polymers.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Materials Science, Ceramics
Rogerio A. Bataglioli, Joao B. M. Rocha Neto, Guilherme B. Calais, Laise M. Lopes, Junko Tsukamoto, Ana P. de Moraes, Clarice W. Arns, Marisa M. Beppu
Summary: The study evaluated iron, copper, silver, and gold salts and their combination with biopolymers to design surfaces with virucidal properties. The alginate-copper sulfate multilayer coatings achieved 99.99% viral inactivation, demonstrating a timely release of copper ions.
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
(2022)
Article
Polymer Science
Cristiana M. P. Yoshida, Murilo S. Pacheco, Mariana A. de Moraes, Patricia S. Lopes, Patricia Severino, Eliana B. Souto, Classius F. da Silva
Summary: The study found that the inclusion of aloe vera altered the color parameters, barrier properties, and mechanical properties of the films. While the concentration of aloe vera did not significantly change film properties, it did increase the stability of the films at lower temperatures. This suggests a crosslinking/complexation between chitosan and aloe vera, providing suitable properties for wound dressing materials.
Article
Public, Environmental & Occupational Health
Wanderson Eduardo Gomes de Souza Coelho, Fabiana Perrechil, Mavilde L. G. Pedreira, Juliana Lima Lopes, Maria Valdenice Lopes dos Santos Nurse, Maria Cristina Gabrieloni, Joao Alessio Perfeito, Mariana Agostini de Moraes, Monica Taminato
Summary: This study evaluated the safety and effectiveness of hydrogen peroxide decontamination method for N95/PFF2 respirators, showing that it did not affect the physical properties of the respirators. The study highlights the importance of exploring decontamination methods to address the scarcity of protective equipment during the COVID-19 pandemic.
AMERICAN JOURNAL OF INFECTION CONTROL
(2021)
Review
Materials Science, Paper & Wood
Jonas Kerwald, Celso Fidelis de Moura Junior, Emanuelle Dantas Freitas, Joao de Deus Pereira de Moraes Segundo, Rodrigo Silveira Vieira, Marisa Masumi Beppu
Summary: Cellulosic materials have gained attention due to their abundance, renewability, and excellent physicochemical properties, while research on nanofibers has been increasing for a wide range of applications. Electrospinning is a promising technique for producing fibers with improved characteristics, although limitations such as poor solubility and inability to melt exist for cellulose and its derivatives. Recent research has focused on exploring the potential applications and processing details of electrospun nanofibers using cellulose and its derivatives as feedstock.
Article
Biochemistry & Molecular Biology
Thiago B. Taketa, Cynthia R. A. Mahl, Guilherme B. Calais, Marisa M. Beppu
Summary: Chitosan-based materials were shown to have enhanced copper ion adsorption capacity in the presence of histidine, with amino acid functionalization promoting an increase in adsorption capacity. The order of addition of histidine to the system influenced the adsorption behavior, and simultaneous addition of chitosan and histidine prevented a decrease in adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Applied
Juliana M. Vaz, Thiago B. Taketa, Jacobo Hernandez-Montelongo, Larissa M. C. G. Fiuza, Cristiano Rodrigues, Marisa M. Beppu, Rodrigo S. Vieira
Summary: Chitosan has shown to be an effective alternative for coating surfaces due to its broad antibacterial spectrum. The study identified a chitosan sample with the best antibacterial effect, which was successfully grafted onto PTFE surfaces. Comparing two different spacer molecules, poly(ethylene glycol) bis (carboxymethyl) ether and poly(ethylene-alt-maleic anhydride), the latter resulted in a coating with more attached chitosan and significantly better antibacterial action against bacterial strains, while also showing compatibility with human fibroblasts in cytotoxicity tests.
JOURNAL OF COATINGS TECHNOLOGY AND RESEARCH
(2022)
Review
Polymer Science
Lucas de Souza Falcao, Deborah Bento Coelho, Priscilla Carvalho Veggi, Pedro Henrique Campelo, Patricia Melchionna Albuquerque, Mariana Agostini de Moraes
Summary: Starch, as a low-cost and abundant raw material, has great potential in replacing synthetic polymers in various applications. It is considered non-toxic, biocompatible, and biodegradable, making it a safe choice for biomedical, food, and packaging industries. This review focuses on the use of starch as a matrix for stabilizing, incorporating or releasing bioactive compounds, and explores its wide range of applications, particularly in the pharmaceutical, packaging, food, and cosmetic industries. The incorporation of microbial bioactive compounds in starch matrices is an unexplored area that requires further investigation.
Article
Polymer Science
Vivian P. de Brito, Mauricio M. de Souza Ribeiro, Juliane Vigano, Mariana A. de Moraes, Priscilla C. Veggi
Summary: Barbatimao bark extract rich in phenolic compounds can be incorporated into silk fibroin hydrogels, and hydrogels containing barbatimao propylene glycol extract show better physical-chemical and structural performance.
Article
Materials Science, Multidisciplinary
Isabella de Lima Oliveira, Lucas de Souza Falcao, Pedro Henrique Campelo, Mariana Agostini de Moraes
Summary: Biopolymers, especially starch, are promising alternatives to plastic due to their non-toxicity and biodegradability. In this study, films were produced using native and modified cassava starch, and their physicochemical properties were analyzed. The thermal treatment of starch had the most significant impact on film characteristics, improving its elongation at break and swelling capability. These modified cassava starch films could be used in various applications, from packaging to biomedical devices.
Article
Biochemistry & Molecular Biology
Rogerio A. Bataglioli, Bruno S. Leao, Carolyn E. Mills, Joao Batista M. Rocha Neto, Thiago B. Taketa, Bradley D. Olsen, Marisa M. Beppu
Summary: The self-assembled layer-by-layer technique is a popular method for engineering biofunctional surfaces. This paper introduces an automated, high-throughput approach to depositing polyelectrolyte multilayers (PEMs) in multiwell plates, allowing for screening of numerous film formulations simultaneously. The method was successfully validated by studying the deposition of different types of films and analyzing their properties. This study demonstrates the challenges associated with predicting the growth and properties of multilayer films and highlights the importance of the high-throughput layer-by-layer method in accelerating film development.
Article
Materials Science, Multidisciplinary
Jose Armando Bertolini Neto, Luisa Storelli dos Reis, Bruno Thorihara Tomoda, Mariana Agostini de Moraes
Summary: The aim of this study was to analyze the behavior of silk fibroin (SF) matrix loaded with charged compounds by preparing SF membranes incorporated with cationic and anionic dyes. The combinatorial effect of pH and ionic strength on the release kinetics was assessed and it was observed that cationic dye had poor interaction with SF and high release rate, while anionic dye presented strong hydrophobic interactions with SF, being retained within the matrix. These findings provide important insights into the behavior of SF in the presence of charged dyes and its potential application in the release of charged drugs.
MRS COMMUNICATIONS
(2022)
Article
Materials Science, Biomaterials
Mohammadreza Kasravi, Alireza Yaghoobi, Tahereh Tayebi, Mahsa Hojabri, Abdolkarim Talebi Taheri, Fatemeh Shirzad, Bahram Jambar Nooshin, Radman Mazloomnejad, Armin Ahmadi, Fatemeh A. Tehrani, Ghasem Yazdanpanah, Mohammad Hadi Farjoo, Hassan Niknejad
Summary: As a promising approach in translational medicine, the decellularization of discarded livers to produce bioscaffolds that support recellularization has potential in overcoming the limitations of conventional liver transplantation. In this study, the researchers investigated the use of matrix metalloproteinase (MMP) inhibition to preserve the extracellular matrix (ECM) during liver decellularization. The results demonstrated that the application of an MMP inhibitor significantly improved the preservation of ECM components and mechanical properties of the bioscaffolds, which supported cell viability and function in vitro. The study also confirmed that the MMP inhibition led to the inhibition of MMP2 and MMP9, providing a novel method to enhance ECM preservation during liver decellularization.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Mohammadsadegh Nadimifar, Weiguang Jin, Clara Coll-Satue, Gizem Bor, Paul Joseph Kempen, Ali Akbar Moosavi-Movahedi, Leticia Hosta-Rigau
Summary: This study presents a metal-phenolic self-assembly approach that can prepare nanoparticles fully made of hemoglobin. The nanoparticles exhibit good oxygen binding and releasing capabilities.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jyoti Kumari, Roel Hammink, Jochem Baaij, Frank A. D. T. G. Wagener, Paul H. J. Kouwer
Summary: Fibrosis is the formation of fibrous connective tissue in response to injury, leading to organ dysfunction. A novel hybrid hydrogel combining synthetic polyisocyanide with hyaluronic acid has been developed, showing strong antifibrotic properties.
BIOMATERIALS ADVANCES
(2024)
Letter
Materials Science, Biomaterials
Melissa Machado Rodrigues, Cristian Padilha Fontoura, Charlene Silvestrin Celi Garcia, Sandro Tomaz Martins, Joao Antonio Pegas Henriques, Carlos Alejandro Figueroa, Mariana Roesch Ely, Cesar Aguzzoli
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jessica Polak, David Sachs, Nino Scherrer, Adrian Suess, Huan Liu, Mitchell Levesque, Sabine Werner, Edoardo Mazza, Gaetana Restivo, Mirko Meboldt, Costanza Giampietro
Summary: Human skin equivalents (HSEs) play a crucial role in tissue engineering. This study introduces a 3D-printed culture insert to apply a static radial constraint on HSEs and examines its effects on tissue characteristics. The results show that the diameter of the culture insert significantly influences tissue contraction, fibroblast and matrix organization, keratinocyte differentiation, epidermal stratification, and basement membrane formation. This study provides important insights for the design of skin tissue engineering.
BIOMATERIALS ADVANCES
(2024)
Review
Materials Science, Biomaterials
Shiliang Chen, Tianming Du, Hanbing Zhang, Jing Qi, Yanping Zhang, Yongliang Mu, Aike Qiao
Summary: This paper reviewed the primary methods for improving the overall properties of biodegradable zinc stents. It discussed the mechanical properties, degradation behavior, and biocompatibility of various improvement strategies. Alloying was found to be the most common, simple, and effective method for improving mechanical properties. Deformation processing and surface modification further improved the mechanical properties and biological activity of zinc alloys. Meanwhile, structural design could endow stents with special properties. Manufacturing zinc alloys with excellent properties and exploring their interaction mechanism with the human body are areas for future research.
BIOMATERIALS ADVANCES
(2024)