期刊
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
卷 28, 期 8, 页码 1250-1254出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2007.11.008
关键词
Keratin; Sponge; Freeze-drying; Porogen; Calcium alginate; Cell scaffold
资金
- Japanese Ministry of Education, Culture, Sports, Science and Technology [16500298]
- Grants-in-Aid for Scientific Research [16500298] Funding Source: KAKEN
Novel fabrication method of highly porous and flexible keratin sponges was developed by combining a particulate-leaching method and a freeze-drying method. Reduced keratin aqueous solution was mixed with dried calcium alginate beads and was lyophilized to give keratin/calcium alginate complex, which was subsequently treated with EDTA solution to leach out calcium alginate beads. The resultant keratin sponge was flexible enough to handle even in dried state because of its quite high porosity (98.9 +/- 0.1%), which was brought about by the large and small pores formed by the elimination of calcium alginate beads and water. The sponge supported the attachment and the proliferation of mouse fibroblast cells. Thus, the keratin sponge given by the present fabrication method afforded one alternative as a cell scaffold for tissue engineering. (C) 2007 Elsevier B.V. All rights reserved.
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