期刊
RESEARCH IN VETERINARY SCIENCE
卷 103, 期 -, 页码 34-43出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.rvsc.2015.09.012
关键词
Mesenchymal stem cell; Canine; Benzo(a)pyrene; Adipogenesis; Environmental carcinogenesis; AhR signaling pathway
资金
- University of Tennessee, Center of Excellence in Livestock Diseases and Human Health [R181701106, R181721276]
Dogs and their owners share the same environment and are subjected to similar environmental risk factors for developing breast cancer. Adipose tissue-derived mesenchymal stem cells (ADMSCs) may affect development and progression of breast cancer. In this study, we evaluated the effects of environmental carcinogen benzo(a)pyrene (BaP) on proliferation and differentiation of ADMSCs isolated from dogs. We characterized eight canine ADMSC lines and studied the effects of BaP on cell proliferation and differentiation. BaP did not inhibit cell proliferation of ADMSCs; however, BaP significantly inhibited differentiation potential of ADMSCs into adipocytes. BaP down-regulated AhR protein levels; however, increased its translocation from the cytoplasm to nucleus and suppressed PPAR gamma expression during adipogenesis. BaP increased the expression of AhR signaling pathway protein, cytochrome P450 (CYP1A1) in ADMSCs. Our data suggest that canine ADMSCs are susceptible to the environmental carcinogen BaP through AhR and PPAR gamma signaling pathways and may contribute to canine mammary carcinogenesis. (C) 2015 Elsevier Ltd. All rights reserved.
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