期刊
MASS SPECTROMETRY REVIEWS
卷 27, 期 4, 页码 331-353出版社
WILEY
DOI: 10.1002/mas.20166
关键词
cyclic nucleotides; cAMP; cGMP; protein kinase A (PKA); protein kinase G (PKG); A-kinase anchoring proteins (AKAPs); chemical proteomics; native mass spectrometry; H/D exchange MS; ion mobility mass spectrometry
类别
Mass spectrometry has evolved in recent years to a well-accepted and increasingly important complementary technique in molecular and structural biology. Here we review the many contributions mass spectrometry based studies have made in recent years in our understanding of the important cyclic nucleotide activated protein kinase A (PKA) and protein kinase G (PKG). We both describe the characterization of kinase isozymes, substrate phosphorylation, binding partners and post-translational modifications by proteomics based methodologies as well as their structural and functional properties as revealed by native mass spectrometry, HID exchange MS and ion mobility. Combining all these mass spectrometry based data with other biophysical and biochemical data has been of great help to unravel the intricate regulation of kinase function in the cell in all its magnificent complexity.
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