期刊
MARINE POLLUTION BULLETIN
卷 88, 期 1-2, 页码 215-223出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.marpolbul.2014.09.002
关键词
Coastal zone; Bacteria; Denitrification; Pyrosequencing; Real-time quantitative PCR (qPCR)
资金
- central basic scientific research project in the Public Welfare for the Scientific Research Institutes [gyk5091301]
- Special Fund for Environmental Protection Research in the Pubic Interest [201309007]
The microbial community and the nirS-and nirK-encoding denitrifiers in the intertidal sediments along Laizhou Bay in China were studied using pyrosequencing and real-time quantitative PCR (qPCR), respectively. There were three primary intertidal zones: Laizhou (La), Weifang Harbor (We), and Dongying (Do). Significant differences in composition and abundances at the different taxonomic levels were observed among the three bacterial communities. The qPCR results indicated that the nirS gene abundance varied from 8.67 x 10(5) to 5.68 x 10(6) copies/g wet weight (ww), whereas the nirK gene abundance varied from 1.26 x 10(5) to 1.89 x 10(6) copies/g ww. The canonical correlation analysis (CCA) indicated that the sand percentage was the most important factor in shaping the bacterial community followed by silt percentage, NO2,- TOC, DO, pH, and clay percentage, whereas the clay percentage, pH, NO3-, DO, NO2-, TOC, silt percentage, and sand percentage were the most important factors associated with regulating the abundance of nirS-and nirK-encoding denitrifiers. (C) 2014 Elsevier Ltd. All rights reserved.
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