期刊
MARINE POLLUTION BULLETIN
卷 62, 期 1, 页码 133-139出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.marpolbul.2010.08.022
关键词
Sphingomonas; Mycobacterium; Mangrove sediment; PAH-degrading bacteria; PAHs; Microbial community structure
资金
- City University of Hong Kong [7008036]
- Natural Research Fund of Guangdong Province, Guangdong [9351064101000001]
- Fundamental Research Funds for the Central Universities of China [2009ZM0034]
The change in community diversity and structure of the indigenous, dominant, polycyclic aromatic hydrocarbon (PAH)-degrading bacterial genera, Sphingomonas and Mycobacterium, due to contamination in the environment is not very well known. A combination of PCR-DGGE with specific primers and a cultivation-dependent microbiological method was used to detect different populations of Sphingomonas and Mycobacterium in mangrove sediments. The structure of the entire bacterial community (including Sphingomonas) did not show a shift due to environmental contamination, whereas the diversity of Mycobacterium populations in mangrove sediments with higher PAH contamination increased from exposure between Day 0 and Day 30. The isolated Mycobacterium strains migrated to the same position as the major bands of the bacterial communities in Mycobacterium-specific DGGE. A dioxygenase gene system, nidA, which is commonly found in PAH-degrading Mycobacterium strains, was also detected in the more highly contaminated sediment slurries. The present study revealed that Mycobacterium species were the dominant PAH-degraders and played an important role in degrading PAHs in contaminated mangrove sediments. (C) 2010 Elsevier Ltd. All rights reserved.
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