期刊
MARINE GENOMICS
卷 15, 期 -, 页码 75-84出版社
ELSEVIER
DOI: 10.1016/j.margen.2014.03.001
关键词
Quantitative real time RT-PCR; Internal reference; Housekeeping gene; Oplegnathus fasciatus
资金
- National Basic Research Program of China [2012CB114404]
- 863 High Technology Project of the Chinese Ministry of Science and Technology [2012AA10A413-1]
- Taishan Scholar Program of Shandong Province
Rock bream Oplegnathus fasciatus is an important economic fish species. In this study, we evaluated the appropriateness of six housekeeping genes as internal controls for quantitative real-time PCR (RT-qPCR) analysis of gene expression in rock bream before and after pathogen infection. The expression of the selected genes in eight tissues infected with Vibrio alginolyticus or megalocytivirus was determined by RT-qPCR, and the PCR data were analyzed with geNorm and NormFinder algorithms. The results showed that before pathogen infection, mediator of RNA polymerase II transcription subunit 8 and beta-actin were ranked as the most stable genes across the examined tissues. After bacterial or viral infection, the stabilities of the housekeeping genes varied to significant extents in tissue-dependent manners, and no single pair of genes was identified as suitable references for all tissues for either of the pathogen stimuli. In addition, for the majority of tissues, the most stable genes during bacterial infection differed from those during viral infection. Nevertheless, optimum reference genes were identified for each tissue under different conditions. Taken together, these results indicate that tissue type and the nature of the infectious agent used in the study can all influence the choice of normalization factors, and that the optimum reference genes identified in this study will provide a useful guidance for the selection of internal controls in future RT-PCR study of gene expression in rock bream. (C) 2014 Elsevier B.V. All rights reserved.
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