Article
Biology
Zhihong Gong, Rui Shi, Songlin Chen, Na Wang
Summary: In this study, the circular RNAs (circRNAs) and their competing endogenous RNA (ceRNA) network potentially regulating sexual size dimorphism (SSD) in Chinese tongue sole were identified for the first time. Several circRNAs were found to regulate gene expression and potentially affect the SSD of Chinese tongue sole.
Article
Environmental Sciences
Jialin Wang, Qian Yang, Yuanri Hu, Wenteng Xu, Yingming Yang, Songlin Chen, Na Wang
Summary: This study utilized transcriptomic analysis on Chinese tongue sole to investigate the regulatory roles of long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) in sexual size dimorphism (SSD). The study revealed the potential involvement of these ncRNAs in regulating growth-related pathways in the gonad and muscle tissues, as well as their clustering patterns through weighted gene co-expression network analysis. The findings suggest a novel regulatory network of lncRNA-miRNA-mRNA in SSD of C. semilaevis.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Fisheries
Daiqiang Zhang, Bao Shi, Peng Shao, Changwei Shao, Chongnv Wang, Jing Li, Xuezhou Liu, Xiaodong Ma, Xinyu Zhao
Summary: This study comprehensively analyzed the transcriptomic modulation of the brain-pituitary-gonad (BPG)-liver axis related to ovary maturation in Cynoglossus semilaevis. Through miRNA sequencing, a large number of miRNAs were identified, some of which were found to simultaneously regulate multiple mRNAs and participate in various biological functions. Important mRNAs related to reproduction were screened based on target gene relationships. KEGG pathway analysis revealed signaling pathways related to steroid hormone biosynthesis, oocyte meiosis, and other reproductive processes. RT-qPCR confirmed the expression patterns of differentially expressed miRNAs and validated the reliability of the sequencing data. Furthermore, the binding site between miR-186-x and igf2r was validated for the first time.
Article
Biotechnology & Applied Microbiology
Na Wang, Jin Gao, Yang Liu, Rui Shi, Songlin Chen
Summary: This study reveals the key regulatory factors associated with female-biased sexual size dimorphism in Chinese tongue sole through GWAS and DAP-seq, identifies the relevant SNP and genes, and explores the regulatory network of zbed1 in this species.
Article
Microbiology
Qian Zhou, Xue Zhu, Yangzhen Li, Pengshuo Yang, Shengpeng Wang, Kang Ning, Songlin Chen
Summary: In this study, the host-microbiome interactions in regulating vibriosis resistance were investigated using C. semilaevis as a research model. The differences in intestinal microbiome on taxonomic and functional levels were found to influence the host gene expressions, and the intestinal microbiome might control the host immunological homeostasis and inflammation to enhance vibriosis resistance through the microbe-intestine-immunity axis.
Article
Fisheries
Qian Yang, Xihong Li, Rui Shi, Peng Cheng, Na Wang, Songlin Chen
Summary: Insulin-like growth factor binding protein 7 (igfbp7) was found to be female-biased expressed in the gonad of Chinese tongue sole (Cynoglossus semilaevis). The expression level of igfbp7 was highest at 6 months during the different gonad developmental stages. In addition, igfbp7 exhibited lower methylation levels in females. Furthermore, igfbp7 was found to play a role in the activation of the NF-kappa B signaling pathway.
Article
Biotechnology & Applied Microbiology
Genmei Lin, Dong Gao, Jianguo Lu, Xiaowen Sun
Summary: This study investigated the gene expression patterns during gonad differentiation in the half-smooth tongue sole, revealing differentially expressed genes associated with sex differentiation and gonad development. Trend analysis showed that gene expression patterns varied over time, with either common features or significant sexual dimorphism. Transcript structure analyses identified both sex and time differences among samples, clarifying the significant sexual differences during early development and providing insight into sex differentiation and development mechanisms.
MARINE BIOTECHNOLOGY
(2021)
Article
Fisheries
Xihong Li, Qian Yang, Rui Shi, Xiwen Xu, Zhangfan Chen, Songlin Chen, Na Wang
Summary: In this study, the researchers found differential expression of igfbp genes in Chinese tongue sole, which is an economically important marine flatfish. These genes play a role in growth and development, and interact with key molecules involved in these processes. The study also revealed differences in gene expression and DNA methylation patterns in different tissues, as well as the differential effects of estradiol and testosterone on igfbp gene expression.
Article
Environmental Sciences
Ying Zhu, Rui Shi, Qian Yang, Mengqian Zhang, Songlin Chen, Na Wang
Summary: The study identified two nipbla genes in Chinese tongue sole and revealed sex-biased expression patterns of different nipbla-w and nipbla-z transcripts in female and male tissues. Functional analysis through RNA interference and prediction of transcription factors showed the important role of these genes in growth regulation.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Fisheries
Yangzhen Li, Yuanri Hu, Yingming Yang, Jiayu Cheng, Xiangming Cheng, Songlin Chen
Summary: Genetic variation for phenotypic female proportion (FP) in tongue sole has been confirmed, with moderately positive genetic correlations between FP and growth traits, indicating the feasibility of joint improvement through selective breeding.
Article
Reproductive Biology
Rui Shi, Xihong Li, Peng Cheng, Qian Yang, Zhangfan Chen, Songlin Chen, Na Wang
Summary: Sexual size dimorphism (SSD) is common in various species, with oocyte secreted factors like gdf9 and bmp15 showing differential expression in the female gonad of Chinese tongue sole. This expression may be partly influenced by DNA methylation status.
Article
Biochemistry & Molecular Biology
Mengqian Zhang, Qian Yang, Rui Shi, Jialin Wang, Ziwei Zhang, Yingming Yang, Wenlong Li, Songlin Chen, Na Wang
Summary: Sexual size dimorphism (SSD) is a common phenomenon in various species and is related to feeding efficiency, energy allocation, sex steroids, and reproductive endocrine axes. This study investigated the effects of 17 beta-estradiol (E2) and testosterone (T) on growth traits in Chinese tongue soles and examined the genetic regulation using several growth-related genes. The results showed that both E2 and T had inhibitory effects on the growth performance of different sexes of C. semilaevis. The expression of certain genes associated with growth and hormones was also affected by the treatments. Overall, this study provides important insights into the role of sex steroids in flatfish SSD.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Na Wang, Yongsheng Tian, Jingjing Zhang, Zhentong Li, Meiling Cheng, Yuping Wu
Summary: The starry flounder, a flatfish species with female-biased growth advantage, has been found to have a significant number of differentially expressed genes between male and female samples. Activation of glycolysis pathway in female muscle tissues contributes to sexual size dimorphism in flatfish.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2021)
Article
Endocrinology & Metabolism
Allison J. Richard, Hardy Hang, Timothy D. Allerton, Peng Zhao, Tamra Mendoza, Sujoy Ghosh, Carrie M. Elks, Jacqueline M. Stephens
Summary: STAT5 is important for adipocyte differentiation and its deletion leads to increased subcutaneous and inguinal fat tissue, with no effect on visceral or gonadal fat tissue. Adipocyte size increases without changes in lipolysis, and adipose gene expression and whole-body energy expenditure are sex-dependent.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Fisheries
Bin Wang, Aijun Cui, Yaxing Zhang, Yongjiang Xu, Weixin Wang, Yan Jiang, Xuezhou Liu
Summary: This study reveals the existence of the tac3 gene in a flatfish species, suggesting its potential involvement in the control of reproduction in the half-smooth tongue sole. The tac3 gene was found to be highly expressed in the brain, intestine, and gonads of both sexes, with the highest levels observed in the hypothalamus. Additionally, the expression of tac3 mRNA in the pituitary and ovary varied during ovarian maturation, indicating a potential role in the reproductive process.
AQUACULTURE REPORTS
(2021)
Article
Biology
Yi Ya Wang, Li Xue Sun, Jia Jie Zhu, Yan Zhao, Hui Wang, Hong Jun Liu, Xiang Shan Ji
JOURNAL OF THERMAL BIOLOGY
(2017)
Article
Fisheries
Zhi Lei Yao, Yan Zhao, Hong Yu Ma, Hong Jun Liu, Hui Wang, Xiang Shan Ji
AQUACULTURE INTERNATIONAL
(2018)
Article
Biochemistry & Molecular Biology
Li Xue Sun, Jian Teng, Yan Zhao, Ning Li, Hui Wang, Xiang Shan Ji
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Multidisciplinary Sciences
Li-Xue Sun, Yi-Ya Wang, Yan Zhao, Hui Wang, Ning Li, Xiang Shan Ji
Article
Cell Biology
Yan Zhao, Yuan Mei, Hong Ju Chen, Li Tao Zhang, Hui Wang, Xiang Shan Ji
PHYSIOLOGICAL GENOMICS
(2019)
Article
Fisheries
Zhi Lei Yao, Yan Zhao, Hui Wang, Hong Ju Chen, Xiang Shan Ji
Article
Fisheries
Yan Zhao, Hong Ju Chen, Yao Yao Wang, Yuan Mei, Li Bo Huang, Hui Wang, Xiang Shan Ji
Article
Biotechnology & Applied Microbiology
Jian Teng, Yan Zhao, Hong Ju Chen, Hui Wang, Xiang Shan Ji
MARINE BIOTECHNOLOGY
(2020)
Article
Genetics & Heredity
Min Zhou, Zhilei Yao, Min Zhao, Qingfeng Fang, Xiangshan Ji, Hongju Chen, Yan Zhao
Summary: This study found that high-temperature treatment during critical thermosensitive periods can induce sex reversal in Nile tilapia, and the Jarid2b gene plays a crucial role in this process.
Article
Fisheries
Jian Teng, Meng Yao Cui, Yan Zhao, Hong Ju Chen, Wen Jing Du, Liang Yi Xue, Xiang Shan Ji
Summary: This study cloned the coding sequence of Canccrp1 from northern snakehead and found that its expression was higher in immune-related organs. After infection with Nocardia seriolae, the mRNA and protein expression of Canccrp1 increased gradually, along with changes in the number and localization of NCCs. These results suggest potential roles for fish NCCs in the antimicrobial infection process in fish.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Fisheries
Yan Zhao, Hong-ju Chen, Chun-Ge Li, Hui Wang, Hong Jun Liu, Xiang-Shan Ji
AQUACULTURE REPORTS
(2016)