期刊
MARINE GENOMICS
卷 2, 期 3-4, 页码 169-181出版社
ELSEVIER
DOI: 10.1016/j.margen.2009.10.001
关键词
Hemoglobin; Hemoglobin variability; PCR errors; beta/alpha globin genes; Genomic exploration; Linkage disequilibrium
资金
- Icelandic Research Fund
Allozyme variation in Atlantic cod hemoglobins shows various signs of natural selection. We report a genomic exploration of globin genes in this non-model organism. Applying a PCR based strategy with a strict criterion of phylogenetically informative sites we estimate the number of linked beta and alpha globin genes. We estimate PCR error rate by PCR of cloned DNA and recloning and by analysis of singleton variable sites among clones. Based on the error rate we exclude variable sites so that the remaining variation meets successively stricter criteria of doubleton and triplet variable site. Applying these criteria we find ten clusters of linked beta/alpha globin genes in the genome of Atlantic cod. Six variable amino acid changes in both genes were found in linkage disequilibrium with silent nucleotide substitutions. A phylogenetic tree, based on our strictly phylogenetically informative sites among 57 clones from 19 individuals, is split into two major branches by an amino acid change in a beta gene. This change is supported by extensive linkage disequilibrium between the amino acid change and numerous other phylogenetically informative silent nucleotide sites. The different gene sets in the genome may represent different loci encoding different globins and/or allelic variation at some loci. (C) 2009 Elsevier B.V. All rights reserved.
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