4.1 Review

The future of three-dimensional microscopic imaging in marine biology

期刊

MARINE ECOLOGY-AN EVOLUTIONARY PERSPECTIVE
卷 32, 期 4, 页码 438-452

出版社

WILEY
DOI: 10.1111/j.1439-0485.2011.00442.x

关键词

Light sheet microscopy; microscopy; MRI; optical projection tomography; three-dimension; X-ray microtomography

资金

  1. European Synchrotron Radiation Facility Postdoctoral Project through allocation of beam time

向作者/读者索取更多资源

Measuring biodiversity in the oceans and its modifications with time or during climate change requires the accurate description of plankton organisms from viruses to fishes. However, our taxonomic knowledge of this 70% of the Earth is extremely limited. The ultimate way to perform large-scale taxonomical analysis is to achieve full 3D imaging of every specimen collected. Novel 3D imaging techniques are undoubtedly at the forefront of such efforts, and will provide an important tool to identify, classify and generate reference models (or 3D gold standards) for rapid recognition and classification of plankton organisms during community or basin studies. This review gives an overview of recently developed 3D imaging techniques and discusses their limitations and promises.

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