期刊
MARINE BIOTECHNOLOGY
卷 13, 期 4, 页码 706-712出版社
SPRINGER
DOI: 10.1007/s10126-010-9332-9
关键词
BAC; Common carp; Genome; Comparative mapping; Synteny
资金
- National Department Public Benefit Research Foundation [200903045]
- 948 program of Minister of Agriculture of China [2010-Z11]
- Research Foundation of Chinese Academy of Fishery Sciences [2009B002]
A bacterial artificial chromosome (BAC) library of common carp Cyprinus carpio L. was constructed as a part of ongoing common carp genome project, which is aiming assembly of common carp genome. The library, containing a total of 92,160 BAC clones with an average insert size of 141 kb, was constructed into the restriction site of Hind III on BAC vector CopyControl pCC1BAC, covering 7.7 X haploid genome equivalents. Three dimension pools and superpools of the BAC library were established and 23 positive clones of 14 targets were identified from one-fifth of the BAC library. Pilot project of BAC end sequencing was conducted on 2,688 BAC ends from 1,344 clones and harvested 2,522 high-quality Q20 sequences with average length of 677 bp. The sequencing success rate was 93.8% and pair-end success rate was 92.3%. A total of 212 microsyntenies had been established between common carp and zebrafish genomes as a trial for genome-wide comparative genomics in these two closely related species.
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