Influences of Culture Temperature on the Growth, Lipid Content and Fatty Acid Composition of Aurantiochytrium sp Strain mh0186

The growth, lipid content, and fatty acid composition of Aurantiochytrium sp. strain mh0186 at different temperatures were investigated. Strain mh0186 grew well at 15-30A degrees C, but weakly at 10A degrees C. The biomass at 15-30A degrees C was significantly higher than at 10 and 35A degrees C, and the total lipid at 15-35A degrees C was significantly higher than that at 10A degrees C. The amount of DHA in the total fatty acid was highest at 10A degrees C and decreased in response to temperature increase. The content of DHA (mg/g-dry cell weight) at 15-30A degrees C were significantly higher than those at 35A degrees C and those at 15-25A degrees C were significantly higher than those at 10 and 35A degrees C. The DHA yield at 15-35A degrees C was significantly higher than those at 10 and 35A degrees C. Unsaturation of fatty acid was regulated by temperature and was enhanced in response to temperature decrease. The ratio of DHA to DPA varied at different temperatures.