期刊
MARINE BIOLOGY RESEARCH
卷 7, 期 3, 页码 304-309出版社
TAYLOR & FRANCIS AS
DOI: 10.1080/17451000.2010.515230
关键词
ELISA; Follicle-stimulating hormone (FSH); immunohistochemistry; Marsupenaeus japonicus; neuroendocrine; reproduction
资金
- National Natural Science Foundation of China [30300269, 40776084]
- Fujian Province University
The identification of vertebrate follicle-stimulating hormone (FSH) may further elucidate the reproductive mechanisms in the prawn Marsupenaeus japonicus. By immunohistochemistry, FSH-like neurons were detected in the brain and thoracic ganglia of the female kuruma prawn. By enzyme-linked immunosorbent assay (ELISA), concentrations of an FSH-like substance were determined in the brain, thoracic ganglia and haemolymph during the maturation of the ovaries. Peak levels of an FSH-like substance culminated in the brain and thoracic ganglia during the exogenous vitellogenic stage, while the maximum level of the FSH-like substance was detected in the haemolymph. Our results indicated that the vertebrate FSH-like substances are present in M. japonicus, suggesting they may be involved in the ovarian maturation.
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