期刊
MARINE BIOLOGY
卷 157, 期 10, 页码 2279-2287出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00227-010-1495-4
关键词
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资金
- Ministry of Education, Culture, Sports, Science and Technology, Japan [20570093]
- University of the Ryukyus
- Grants-in-Aid for Scientific Research [20570093] Funding Source: KAKEN
The polyp (scyphistoma) of the jellyfish Cassiopea andromeda reproduces asexually repeatedly, while the medusa, the sexually reproducing stage, exhibits a relatively shorter life span. As a first step to understand the mechanism behind the differences in the life spans of the polyp and medusa stages of the jellyfish, we compared the lengths of the telomere region of one targeted chromosome between the polyp and medusa stages using a modified single telomere length assay (STELA). The double-stranded regions of the telomeres were amplified by PCR, and the average length of the PCR products was estimated by densitometry analysis of the gel smear. Chromosomes within cells of the bell region of the medusa were characterized by longer telomeres than those of polyps, asexual propagules, or other regions of the medusa. This is the first study to estimate the telomere lengths of targeted chromosomes in a cnidarian and opens a way to understand the mechanism underlying different life spans of the polyp and medusa stages.
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