期刊
MAGNETIC RESONANCE MATERIALS IN PHYSICS BIOLOGY AND MEDICINE
卷 25, 期 6, 页码 479-485出版社
SPRINGER
DOI: 10.1007/s10334-012-0315-x
关键词
Lipid droplets; Size; NMR lipid signal
资金
- Medical Research Council, UK [G0601327]
- Andrew McCartney Trust
- Birmingham Children's Hospital Research Foundation
- Brain and Nervous System Tumor Research Fund
- Medical Research Council [G0601327] Funding Source: researchfish
- MRC [G0601327] Funding Source: UKRI
Cytoplasmic lipid droplets (LDs) are dynamic cellular organelles; their accumulation is associated with several cellular processes, such as cell proliferation, apoptosis and necrosis. H-1 Nuclear Magnetic Resonance (NMR) spectroscopy detects resonances from lipids present in cytoplasmic (LDs); an understanding of the relationship between LD characteristics and NMR lipid signals is important. In this study, five nervous system cancer cell lines were investigated. Nile red staining was used to measure the diameter of LDs. High-resolution magic angle spinning NMR (HR-MAS) was performed on harvested cell pellets to quantify the patterns of lipid signals. LDs were present in all five cell lines with different morphology. An average LD diameter of approximately 0.2 mu m was found in all cell types. Diameter of the largest LDs varied across the cell lines. The intensity of NMR lipid signals varied greatly between cell types, and a good correlation was found between total volume of LDs and the proton NMR lipid signal intensity at 0.9 and 1.3 ppm. The correlation implied that little NMR signal is detected from LDs of diameters less than approximately 0.34 mu m, most likely due to restriction of rotational motion of the lipids.
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