Review
Food Science & Technology
Marlene Machado, Helena Ferreira, M. Beatriz P. P. Oliveira, Rita C. Alves
Summary: Consumers' demand for nutritious and healthy foods has driven the development of prebiotic foods. In the coffee industry, the waste/by-products generated from the transformation of cherries into roasted beans can be used as sources of prebiotic ingredients. Coffee by-products contain dietary fiber and other components that can improve gut health and stimulate beneficial bacteria, making them potential prebiotic ingredients. However, further in vivo studies are needed to validate these findings obtained in vitro. This review highlights the potential of coffee by-products in the development of functional foods, contributing to sustainability, circular economy, food security, and health.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Chemistry, Applied
Fareeya Kulapichitr, Chaleeda Borompichaichartkul, Mingchih Fang, Inthawoot Suppavorasatit, Keith R. Cadwallader
Summary: The drying process affects the qualitative indicators of green coffees to varying degrees, with sun drying and heat pump drying resulting in comparable levels of CGAs and antioxidant activities. Specific CGAs, antioxidant activities, and color parameters are correlated among coffees. Heat pump drying at 50 degrees C is a viable alternative and possibly superior to sun drying for preserving certain desirable chemical and physical characteristics of green coffee, according to results from CGAs contents and PCA analysis.
Article
Chemistry, Applied
Joanna Grzelczyk, Dominik Szwajgier, Ewa Baranowska-Wojcik, Grazyna Budryn, Malgorzata Zaklos-Szyda, Bozena Sosnowska
Summary: The aim of this study was to investigate the release of chlorogenic acids (CHAs) from coffee extracts during digestion in different sections of the gastrointestinal tract, with and without the presence of probiotics. The concentration of free CHAs was found to be the lowest in the stomach and highest in the large intestine, and probiotics significantly increased the release of CHAs, particularly in the colon. Furthermore, digested coffee extracts showed cytoprotective potential against oxidative stress.
Article
Food Science & Technology
Minaleshewa Atlabachew, Atakilt Abebe, Tessera Alemneh Wubieneh, Yilak Tefera Habtemariam
Summary: The proposed method utilizes salting-out assisted liquid-liquid extraction for simultaneous determination of trigonelline, caffeine, and chlorogenic acid in green coffee bean extract. It shows acceptable linearity and excellent correlation compared to RP-HPLC-DAD, making it suitable for quality control and geographical origin traceability studies in the coffee industry.
FOOD SCIENCE & NUTRITION
(2021)
Review
Food Science & Technology
Sara E. Yeager, Mackenzie E. Batali, Jean-Xavier Guinard, William D. Ristenpart
Summary: This study conducted a systematic review and meta-analysis on the concentrations of organic acids (OAs) and chlorogenic acids (CGAs) in different types of coffee at various roast levels. The results showed that roasted robusta coffee contained more acidic compounds than arabica, and the concentration of CGAs decreased as the roasting level increased. Moreover, the increase in certain OAs with roast level might play a significant role in the sensory profile of dark roast coffees.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Food Science & Technology
Marlene Machado, Liliana Espirito Santo, Susana Machado, Joana C. Lobo, Anabela S. G. Costa, Maria Beatriz P. P. Oliveira, Helena Ferreira, Rita C. Alves
Summary: This study analyzed the bioactive potential and chemical composition of different coffee by-products and found that they are rich in nutrients, caffeine, and chlorogenic acids. Some by-products also exhibited antioxidant activity. Therefore, these by-products can be used as functional ingredients in various industries, contributing to the sustainability of the coffee industry.
Article
Food Science & Technology
Busra Acikalin, Nevin Sanlier
Summary: Coffee, which has been consumed worldwide for its stimulating effect and distinctive taste, is now recognized for its positive effects on health, particularly on the immune system. Compounds in coffee like caffeine, chlorogenic acids, and diterpenoid alcohols stimulate the immune system due to their antioxidant and anti-inflammatory effects. Caffeine and polyphenols in coffee are considered the main agents with immunomodulatory characteristics, depending on the daily dose of consumption.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2021)
Article
Food Science & Technology
Atita Panyathep, Khanittha Punturee, Teera Chewonarin
Summary: Inflammatory response caused by bacterial infection after colorectal cancer surgery is a major concern. Green coffee bean extract, particularly the ethyl acetate insoluble fraction (EIF) containing chlorogenic acid (CGA), was found to inhibit TLR4 expression and NF-κB activation, resulting in the suppression of inflammatory response and CRC metastasis in colon cancer cells. Therefore, CGA-rich fraction from green coffee bean extract can be developed as an adjunctive treatment for colorectal cancer surgery to enhance therapeutic efficacy and survival rate.
Article
Biochemical Research Methods
Alla Yegorova, Yuliia Skrypynets, Inna Leonenko, Axel Duerkop
Summary: This study presents a simple, sensitive, and validated method for determining the content of chlorogenic acid in green and roasted coffee, without the need for chromatographic separation. The method utilizes luminescence intensity quenching to measure the concentration of chlorogenic acid. The results showed that the method is effective and can be applied for quality control of chlorogenic acid in coffee.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Doaa M. Abdel-Mohsen, Ahmed M. A. Akabawy, Manal F. El-Khadragy, Ahmed Abdel E. Moneim, Hatem K. Amin
Summary: The study investigated the therapeutic potential of green coffee bean extract (GCBE) in the treatment of diabetic hepatic damage induced by a high-fat diet (HFD) and streptozotocin (STZ) administration. A newly stabilized in vivo obese diabetic animal model was created to examine the dose-dependent effect of GCBE in protecting against oxidative stress-induced hepatic damage. The results showed that GCBE improved various parameters, reversed liver changes, and enhanced insulin signaling pathways, indicating its potential in repairing hepatic oxidative damage caused by HFD and STZ.
JOURNAL OF FOOD BIOCHEMISTRY
(2023)
Article
Food Science & Technology
Ruihong Dong, Mengting Zhu, You Long, Qiang Yu, Chang Li, Jianhua Xie, Yousheng Huang, Yi Chen
Summary: This study evaluated the chemical compositions of green coffee beans and their correlation with thermal contaminants in roasted coffee. Positive correlations were found between the formation of 5-HMF, furan, 2-and 3-methylfuran and various compounds in green coffee beans. Additionally, significant positive correlations were observed between acrylamide levels and several compounds in green beans. Negative correlations were found between certain compounds and active constituents, and slight negative relationships were observed between acrylamide and certain compounds. This study provides valuable insights for selecting coffee beans with high nutrition and low chemical risks.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Food Science & Technology
Yan-Han Lin, Hsiao-Wen Huang, Chung-Yi Wang
Summary: The study compared the extraction of bioactive ingredients from green coffee beans using high pressure-assisted extraction (HPE) and traditional heat reflux (HR) extraction methods. The results showed that HPE had significantly higher extraction rates of chlorogenic acid and caffeine compared to the traditional method. HPE also exhibited excellent antioxidant and antibacterial activities under optimal extraction conditions.
FOOD AND BIOPROCESS TECHNOLOGY
(2022)
Article
Food Science & Technology
Vaibhavi A. Pimpley, Pushpa S. Murthy
Summary: The study explored the integrated approach of green technologies for extracting nutraceuticals from green coffee beans, showing that steam-assisted extraction and enzyme-assisted extraction combined can significantly increase the polyphenols and flavonoids, as well as enhance the bio-accessibility of bio-active compounds.
Article
Multidisciplinary Sciences
Graziella R. Molska, Lyvia Izaura G. Paula-Freire, Marna E. Sakalem, Daniele O. Kohn, Giuseppina Negri, Elisaldo A. Carlini, Fulvio R. Mendes
Summary: Epidemiological studies have shown a negative correlation between coffee consumption and the development of Parkinson's disease. The effects of green coffee extracts on rodents with induced disorders suggest a potential pro-dopaminergic action that requires further investigation to understand the underlying mechanisms.
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
(2021)
Article
Food Science & Technology
Julia Montenegro, Lauriza Silva dos Santos, Rodrigo Goncalves Gusmao de Souza, Larissa Gabrielly Barbosa Lima, Daniella Santos Mattos, Bruna Prunes Pena Baroni Viana, Ana Clara Santos da Fonseca Bastos, Leda Muzzi, Carlos Adam Conte-Junior, Etel Rodrigues Pereira Gimba, Otniel Freitas-Silva, Anderson Junger Teodoro
Summary: Research has shown that green and light roasted coffee extracts exhibited the highest antioxidant activity. While caffeine content was not affected by roasting, chlorogenic acid degraded with temperature, and caffeic acid increased in light roasted but decreased in medium and dark roasted coffee. Green and light roasted coffee extracts demonstrated greater inhibition of cell viability, cell cycle arrest, and apoptotic cell death compared to medium and dark roasted extracts, suggesting a potential preventive effect against prostate cancer.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Optics
Zhenhua Du, Feifei Wei, Jiaxin He
Summary: This work designs a new type of photonic crystal fiber using tellurite glass as the material. The finite element method is used to study its features, including dispersion, birefringence, and nonlinearity. The simulation results show that the designed photonic crystal fiber has a high birefringence and nonlinear coefficient, and can generate a wide spectrum supercontinuum at a specific pumping wavelength. These features may be utilized in optical fiber sensing and dispersion compensation.
JOURNAL OF OPTICS-INDIA
(2023)
Article
Chemistry, Multidisciplinary
Chao Li, Xin Gao, Hongbin Qi, Wei Zhang, Lei Li, Cancan Wei, Meijing Wei, Xiaoxuan Sun, Shusen Wang, Liyan Wang, Yingbin Ji, Shuhong Mao, Zhangliang Zhu, Masaru Tanokura, Fuping Lu, Hui-Min Qin
Summary: In this study, a genetically encoded biosensor based on a D-allulose-responsive transcriptional regulator was redesigned for real-time monitoring of D-allulose. Furthermore, an ultrahigh-throughput droplet-based microfluidic screening platform was constructed for the directed evolution of ketose 3-epimerases (KEases). Structural analysis revealed a lid conformation in Sinorhizobium fredii D-allulose 3-epimerase (SfDAE) that regulates substrate recognition, and through rational design and directed evolution, a mutant M3-2 with 17-fold enhanced catalytic efficiency was identified. This research provides a paradigm for the design and optimization of biosensor-based microdroplet screening platforms.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Biotechnology & Applied Microbiology
Feifei Wei, Zhiying Yan, Xianming Zhang, Yaoyao Wang, MiaoJing Wang, Yingwei Zhu, Kequn Xu
Summary: This study aimed to investigate the inhibitory effect of LNCNA-NEA1 on pancreatic cancer development and progression via spongiosa miR-146b-5p/TRAF6. The expression level of NEAT1 in cancerous and adjacent non-cancerous tissues was detected using real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR). Cell counting kit-8 (CCK-8) and transwell assays were conducted to determine the effect of LNCNA-NEA1 on the proliferation and migration of pancreatic cancer cells (Panc-1). The results showed that knockdown of nea 1 could inhibit the proliferation, migration and invasion of pancreatic cancer cells by upregulating the level of miR-146b-5p/TRAF6, suggesting that the expression of lnc RNA-nea 1 could be used as an indicator for preoperative diagnosis and postoperative prognosis of pancreatic cancer patients.
BIOTECHNOLOGY AND GENETIC ENGINEERING REVIEWS
(2023)
Article
Oncology
Hidetomo Himuro, Yoshiro Nakahara, Yuka Igarashi, Taku Kouro, Naoko Higashijima, Norikazu Matsuo, Shuji Murakami, Feifei Wei, Shun Horaguchi, Kayoko Tsuji, Yasunobu Mano, Haruhiro Saito, Koichi Azuma, Tetsuro Sasada
Summary: Checkpoint inhibitors have improved the prognosis of NSCLC, but only a limited number of patients benefit from this therapy and predictive biomarkers remain unclear. The study found that higher levels of sPD-L1 before treatment predicted worse PFS and OS in NSCLC patients treated with ICI monotherapy. After treatment, higher levels of sPD-1 were associated with better OS, while higher levels of sPD-L1 were associated with worse PFS and OS.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Zhangmei Hu, Dandan Peng, Feiyue Xing, Xiang Wen, Kun Xie, Xuemei Xu, Hui Zhang, Feifei Wei, Xiaoke Zheng, Meikun Fan
Summary: A highly sensitive liquid freestanding membrane SERS method was developed for quantitative detection of insecticide deltamethrin residues. It showed good accuracy and high sensitivity, and could be applied in household pesticide residues analysis.
Article
Multidisciplinary Sciences
Satoshi Kidokoro, Izumi Konoura, Fumiyuki Soma, Kazuo Shinozaki, Takamasa Suzuki, Masaru Tanokura, Takuya Miyakawa, Takato Imaizumi, Kazuko Yamaguchi-Shinozaki
Summary: Plants respond to severe temperature changes by inducing the expression of numerous genes whose products enhance stress tolerance and responses. DREB1 genes are rapidly and strongly induced by cold stress and their molecular mechanisms are vital for recognizing the initial responses to cold stress in plants.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Cell Biology
Jiaqi Liu, Linna Kong, Wenqing Bian, Xiaona Lin, Feifei Wei, Jun Chu
Summary: circ_0001006 is upregulated in triple-negative breast cancer (TNBC) and is closely associated with patients' histological grade, Ki67 level, and TNM stage. Upregulated circ_0001006 predicts a worse prognosis and high risk in TNBC patients. Silencing circ_0001006 suppresses cell proliferation, migration, and invasion by negatively regulating miR-424-5p.
Article
Immunology
Yimei Wang, Tomohiro Takano, Yingyu Zhou, Rong Wang, Takayuki Toshimitsu, Toshihiro Sashihara, Masaru Tanokura, Takuya Miyakawa, Haruyo Nakajima-Adachi, Satoshi Hachimura
Summary: This study investigated the effects of Lactiplantibacillus plantarum OLL2712 on obesity-induced inflammation. It was found that the LAB strain regulated gut microbiota, suppressed intestinal inflammation, and improved gut barrier function, thereby reducing obesity-related inflammation.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biology
Akira Nozawa, Ryoko Miyazaki, Yoshinao Aoki, Reina Hirose, Ryosuke Hori, Chihiro Muramatsu, Yukinori Shigematsu, Keiichirou Nemoto, Yoshinori Hasegawa, Keiko Fujita, Takuya Miyakawa, Masaru Tanokura, Shunji Suzuki, Tatsuya Sawasaki
Summary: A new gibberellin receptor agonist, diphegaractin, has been identified in this study, which shows gibberellin-like activity in the growth and maturation of grapes, lettuce, Arabidopsis, and citrus fruit. This is the first successful identification of a GA receptor agonist with GA-like activity in agricultural plants using an in vitro molecular-targeted drug screening system.
COMMUNICATIONS BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Satoshi Yamanaka, Hirotake Furihata, Yuta Yanagihara, Akihito Taya, Takato Nagasaka, Mai Usui, Koya Nagaoka, Yuki Shoya, Kohei Nishino, Shuhei Yoshida, Hidetaka Kosako, Masaru Tanokura, Takuya Miyakawa, Yuuki Imai, Norio Shibata, Tatsuya Sawasaki
Summary: Lenalidomide is an effective drug for treating hematological cancers, but has teratogenic effects on fetuses. This study identifies modifications that enhance the selectivity and effectiveness of lenalidomide as a standalone molecular glue or integrated in PROTACs.
NATURE COMMUNICATIONS
(2023)
Article
Oncology
Feifei Wei, Koichi Azuma, Yoshiro Nakahara, Haruhiro Saito, Norikazu Matsuo, Tomoyuki Tagami, Taku Kouro, Yuka Igarashi, Takaaki Tokito, Terufumi Kato, Tetsuro Kondo, Shuji Murakami, Ryo Usui, Hidetomo Himuro, Shun Horaguchi, Kayoko Tsuji, Kenta Murotani, Tatsuma Ban, Tomohiko Tamura, Yohei Miyagi, Tetsuro Sasada
Summary: A machine learning-based platform, CIRI, was developed to predict the ICI response of patients with NSCLC based on peripheral blood cytokine profiles. The CIRI model accurately determines the patients who would benefit from anti-PD-1/PD-L1 therapy with prolonged overall survival, aiding in clinical decision-making.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Article
Biotechnology & Applied Microbiology
Hiroyuki Takeda, Tatsuhiko Ozawa, Hiroki Zenke, Yoh Ohnuki, Yuri Umeda, Wei Zhou, Honoka Tomoda, Akihiko Takechi, Kimiyoshi Narita, Takaaki Shimizu, Takuya Miyakawa, Yuji Ito, Tatsuya Sawasaki
Summary: This study investigated the use of the Japanese topeshark as an immune animal for the development of VNAR antibodies. By injecting Venus fluorescent protein into the sharks, the levels of antigen-specific IgM and IgNAR antibodies in the blood were significantly increased. Phage-display and yeast-display libraries were constructed using RNA from immunized shark splenocytes, and multiple antigen-specific VNAR antibodies were obtained. These antibodies exhibited high affinity and plasticity, and retained their binding activity even after high-temperature or reducing-agent treatment. Deep sequencing analysis revealed distinct enrichment bias in the CDR3-coding sequences between libraries. Overall, the Japanese topeshark shows promise for the development of VNAR antibodies.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Takafumi Hashimoto, Kenji Hashimoto, Hiroki Shindo, Shoko Tsuboyama, Takuya Miyakawa, Masaru Tanokura, Kazuyuki Kuchitsu
Summary: This study reveals a mechanism of Ca2+ and phosphorylation regulating the activity of MpRBOHB, highlighting the importance of Ca2+ binding in this process. This mechanism is likely to be conserved among land plant RBOHs.
PHYSIOLOGIA PLANTARUM
(2023)