Review
Chemistry, Physical
Aaditya S. Deshpande, Wayne Muraoka, Silvana Andreescu
Summary: Electrochemical sensors are effective in detecting reactive oxygen and nitrogen species in biological processes. Recent developments in microelectrode and electrode materials show promise in studying drug efficacy, mitochondrial distress, and endothelial dysfunction. Challenges and opportunities in applying this methodology to study pathophysiology associated with various diseases are discussed.
CURRENT OPINION IN ELECTROCHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Jay L. Zweier, Craig Hemann, Tapan Kundu, Mohamed G. Ewees, Sahar A. Khaleel, Alexandre Samouilov, Govindasamy Ilangovan, Mohamed A. El-Mahdy
Summary: Cytoglobin (Cygb) has potent superoxide dismutase (SOD) function, rapidly dismutating superoxide in cells to protect against oxidative stress. Its role in cellular superoxide metabolism is important due to its ubiquitous expression in cells at micromolar concentrations.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Nutrition & Dietetics
Gabriele Serreli, Micaela Rita Naitza, Sonia Zodio, Vera Piera Leoni, Martina Spada, Maria Paola Melis, Anna Boronat, Monica Deiana
Summary: Ferulic acid and its metabolites show efficacy in combating inflammation by limiting the expression and activity of proinflammatory enzymes, reducing NF-κB translocation, and promoting Nrf2 expression.
Article
Biochemical Research Methods
Carlisle R. DeJulius, Bryan R. Dollinger, Taylor E. Kavanaugh, Eric Dailing, Fang Yu, Shubham Gulati, Angelo Miskalis, Caiyun Zhang, Jashim Uddin, Sergey Dikalov, Craig L. Duvall
Summary: Polymerizing TEMPO is beneficial for improving in vivo efficacy. By copolymerization with the hydrophilic monomer DMA, a series of poly(DMA-co-TEMPO) random copolymers were formed. An optimal composition of 40% TEMPO/60% DMA was identified for good water solubility and O-2(center dot-) scavenging in vitro. In an air pouch model of acute local inflammation, the optimized copolymer showed better performance in scavenging O-2(center dot-), retention, and reduction of TNF alpha levels compared to the free drug and a 100% poly(TEMPO) formulation. Moreover, the optimized copolymer reduced ROS levels after systemic injection in a footpad model of inflammation. These results suggest the potential of polymerizing TEMPO for developing a useful antioxidant polymer formulation for next-generation anti-inflammatory treatments.
BIOCONJUGATE CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
You-Lin Tain, Chien-Ning Hsu
Summary: Nitric oxide (NO) is a gaseous signaling molecule that plays a crucial role in protecting the kidneys. NO can be generated through different pathways, either dependent or independent of NO synthase (NOS). Deficiency of NO is closely associated with chronic kidney disease (CKD). Adverse intrauterine conditions can impact kidney development, increase the risk of CKD, but early reprogramming treatments have the potential to mitigate this risk. This review highlights the relationship between NOS/NO research and renal programming and reprogramming, and suggests potential strategies targeting NO for preventing and treating renal programming.
Article
Cardiac & Cardiovascular Systems
Jody L. Greaney, Erika F. H. Saunders, Lacy M. Alexander
Summary: Activation of NF-kappa B, through increased vascular ROS production, contributes to reduced NO-dependent endothelial dilation in young adults with MDD. Short-term treatment with NF-kappa B inhibitor salsalate improves NO-mediated endothelium-dependent dilation in MDD patients, while acute localized ROS scavenging with apocynin enhances NO-dependent dilation before salsalate administration.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2022)
Article
Plant Sciences
Weronika Skowronska, Sebastian Granica, Monika E. Czerwinska, Ewa Osinska, Agnieszka Bazylko
Summary: The study confirms the anti-inflammatory and antioxidant activity of elderberry leaves, particularly against reactive oxygen species. This suggests that elderberry leaves may be a potential medicinal plant with anti-inflammatory and antioxidant properties.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tsutomu Ishihara, Ken-ichiro Tanaka, Ayaka Takafuji, Keita Miura, Tohru Mizushima
Summary: In this study, the researchers explored the potential use of benziodarone as a therapeutic agent against diseases caused by overproduction of reactive oxygen species (ROS). They screened a library of approved drugs and found that benziodarone reduced superoxide anions produced by leukemia cells without causing cytotoxicity. Further investigation revealed that benziodarone inhibited NADPH oxidases in the plasma membrane and showed preventive effects on murine lung injury caused by lipopolysaccharide (LPS), demonstrating its ROS-reducing activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Mohd Javed Akhtar, Maqusood Ahamed, Hisham Alhadlaq
Summary: The study illustrates that the cytoprotective potential of CeO2 NPs against cytotoxic agents is mediated through signaling effects of O-2(center dot-) and/or NO, promoting antioxidant capacity in cells and enabling them to tolerate exogenously induced toxicity.
Article
Biochemistry & Molecular Biology
Marialucia Gallorini, Monica Rapino, Helmut Schweikl, Amelia Cataldi, Rosa Amoroso, Cristina Maccallini
Summary: The study demonstrates that new iNOS inhibitors selectively reduce cytotoxicity, IL-6 secretion, and LPS-stimulated monocyte migration, indicating promising therapeutic potential.
Article
Pharmacology & Pharmacy
Linhua Wei, Weiwei Chen, Linke Huang, Hui Wang, Yuangang Su, Jiamin Liang, Haoyu Lian, Jiake Xu, Jinmin Zhao, Qian Liu
Summary: This study explored the effects and mechanism of Alpinetin (Alp) on inflammatory osteolysis. The results showed that Alp suppressed the differentiation and function of osteoclasts, reduced reactive oxygen species production, and inhibited the expression of inflammatory genes. Moreover, Alp inhibited the phosphorylation of specific pathways and minimized LPS-induced osteolysis in mice.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Immunology
Ilandarage Menu Neelaka Molagoda, Wisurumuni Arachchilage Hasitha Maduranga Karunarathne, Mi-Hwa Lee, Chang-Hee Kang, Kyoung Tae Lee, Yung Hyun Choi, Seunghun Lee, Gi-Young Kim
Summary: Acertannin (ACTN), a polyphenol with powerful anticancer and antioxidant effects, has been found to have anti-inflammatory effects. ACTN attenuates proinflammatory cytokines and mediators by inhibiting NF-Kappa B activation, reduces ROS production, and disrupts the binding of LPS to the TLR4/MD2 complex. It also inhibits the recruitment of intracellular adaptor proteins and attenuates NF-Kappa B-mediated inflammatory responses.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Review
Food Science & Technology
Joseph Festa, Mariasole Da Boit, Aamir Hussain, Harprit Singh
Summary: Cardiovascular disease is the leading cause of global death, with endothelial dysfunction being a predictor for most CVD. Polyphenols from sources like wine and grape extracts can help prevent endothelial dysfunction, while anthocyanin flavonoids found in berries also improve vascular function.
MOLECULAR NUTRITION & FOOD RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Andrea Pautz, Huige Li, Hartmut Kleinert
Summary: Nitric oxide synthases (NOS) are major sources of nitric oxide (NO) in the vasculature and play a crucial role in regulating vascular tone and blood pressure. While NOS3 is the most important for maintaining vascular tone, evidence suggests that NOS1 and NOS2 also contribute to vascular function. Regulation of NOS expression in the vasculature involves various mechanisms including transcriptional, post-transcriptional, and post-translational processes, particularly in the context of inflammation and innate immune pathways.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2021)
Review
Biochemistry & Molecular Biology
Linda de Bont, Xiujie Mu, Bo Wei, Yi Han
Summary: Hydrogen sulfide (H2S) plays an important role in plant growth and adaptation to abiotic stress. It functions in defense by activating plant hormones and is involved in oxidative stress responses. Additionally, H2S interacts with nitric oxide and reactive oxygen species, regulating oxidative stress responses.
JOURNAL OF GENETICS AND GENOMICS
(2022)
Article
Biochemistry & Molecular Biology
Yasuhiro Ishihara, Sarah Y. Kado, Christiane Hoeper, Shelly Harel, Christoph F. A. Vogel
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Toxicology
Yasuhiro Ishihara, Thomas Haarmann-Stemmann, Norman Y. Kado, Christoph F. A. Vogel
TOXICOLOGICAL SCIENCES
(2019)
Article
Oncology
Christoph F. A. Vogel, Yasuhiro Ishihara, Claire E. Campbell, Sarah Y. Kado, Aimy Nguyen-Chi, Colleen Sweeney, Marius Pollet, Thomas Haarmann-Stemmann, Joseph M. Tuscano
Article
Nanoscience & Nanotechnology
Ririko Nakaoka, Hiroshi Hirata
Article
Biochemical Research Methods
Takahito Yokoyama, Akihiro Taguchi, Harue Kubota, Neil J. Stewart, Shingo Matsumoto, Igor A. Kirilyuk, Hiroshi Hirata
JOURNAL OF MAGNETIC RESONANCE
(2019)
Article
Public, Environmental & Occupational Health
Mayumi Tsuji, Chihaya Koriyama, Yasuhiro Ishihara, Megumi Yamamoto, Kiwako Yamamoto-Hanada, Kumiko Kanatani, Yu Ait Bamai, Kazunari Onishi, Ayako Senju, Shunsuke Araki, Eiji Shibata, Seiichi Morokuma, Masafumi Sanefuji, Hiroshi Kitazawa, Mayako Saito, Masakazu Umezawa, Atsuto Onoda, Koichi Kusuhara, Rie Tanaka, Toshihiro Kawamoto, Hirohisa Saito, Reiko Kishi, Nobuo Yaegashi, Koichi Hashimoto, Chisato Mori, Shuichi Ito, Zentaro Yamagata, Hidekuni Inadera, Michihiro Kamijima, Takeo Nakayama, Hiroyasu Iso, Masayuki Shima, Yasuaki Hirooka, Narufumi Suganuma, Takahiko Katoh
JOURNAL OF EPIDEMIOLOGY
(2019)
Article
Environmental Sciences
Jong In Park, Kwon Choi, Chang Uk Koo, Jeonghun Oh, Hiroshi Hirata, Harold M. Swartz, Sung-Joon Ye
Summary: This study aimed to improve the accuracy of EPR-based in vivo tooth dosimetry by analyzing the relationship between tooth geometry and radiation-induced signals. The research confirmed a linear relationship between simulated RIS and tooth geometric dimensions and developed a method for geometry correction to reduce dosimetric uncertainties. The correction method using enamel volume showed the best accuracy in reducing dosimetric uncertainties resulting from variations in tooth geometric dimensions.
Article
Chemistry, Physical
Neil J. Stewart, Hitomi Nakano, Shuto Sugai, Mitsushi Tomohiro, Yuki Kase, Yoshiki Uchio, Toru Yamaguchi, Yujirou Matsuo, Tatsuya Naganuma, Norihiko Takeda, Ikuya Nishimura, Hiroshi Hirata, Takuya Hashimoto, Shingo Matsumoto
Summary: A cost-effective synthetic pathway for high-yield hydrogenation precursor was developed, with the trans-selectivity of the hydrogenation reaction elucidated using density functional theory simulations. The study successfully demonstrated the generation of hyperpolarized [1-C-13]fumarate through a simple PHIP set-up, allowing for ex vivo detection in murine liver tissue homogenates and in vivo MR spectroscopy and imaging in a murine model of acetaminophen-induced hepatitis.
Article
Biochemistry & Molecular Biology
Kota Kimura, Nami Iguchi, Hitomi Nakano, Hironobu Yasui, Shingo Matsumoto, Osamu Inanami, Hiroshi Hirata
Summary: An accelerated imaging system using EPR and nitroxyl radicals was established for redox-sensitive mapping in a mouse tumor model. Sparse sampling technique was successfully applied to evaluate solution phantom and mouse xenograft models, achieving fourfold acceleration in 3D redox-sensitive mapping compared to conventional methods.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Review
Biochemistry & Molecular Biology
Neil J. Stewart, Tatsuyuki Sato, Norihiko Takeda, Hiroshi Hirata, Shingo Matsumoto
Summary: This article highlights the importance and potential of using C-13 hyperpolarized metabolic tracers for MRI imaging to evaluate redox state. It discusses the challenges and future directions in developing specific C-13 tracer biomarkers for redox state evaluation.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Review
Physics, Atomic, Molecular & Chemical
Hiroshi Hirata, Harold M. Swartz
Summary: This article reviews the current status and challenges of using RF/microwave resonators for electron paramagnetic resonance (EPR) spectroscopy and imaging from the viewpoint of preclinical and clinical applications. Resonator developments are summarized in the contexts of preclinical oximetry and free radical measurements in small rodents, clinical (human) oximetry, and human dosimetry, providing guidelines for resonator development and optimization for specific preclinical and clinical EPR applications.
APPLIED MAGNETIC RESONANCE
(2022)
Article
Physics, Atomic, Molecular & Chemical
Yasuhiro Nakai, Ichiro Yamaguchi, Hiroshi Hirata, Harold M. Swartz, Ann Barry Flood, Benjamin B. Williams, Wilson Schreiber, Minoru Miyake
Summary: The L-band electron paramagnetic resonance (EPR) in vivo dosimetry is a potential method for triage in radiation incidents, but UV exposure can impact the accuracy of EPR dose measurements.
APPLIED MAGNETIC RESONANCE
(2022)
Article
Biochemical Research Methods
Denis A. Komarov, Alexandre Samouilov, Hiroshi Hirata, Jay L. Zweier
Summary: The linearity of magnetic field sweep is crucial for high resolution continuous wave EPR imaging. A method using calibrated harmonics to generate highly linear and symmetrical triangular magnetic field sweeps was introduced. By measuring the frequency response function and adjusting the amplitudes and phases of harmonics, the deviation of magnetic field sweep was reduced successfully.
JOURNAL OF MAGNETIC RESONANCE
(2021)
Article
Chemistry, Analytical
Ririko Nakaoka, Kazuhiro Kato, Kumiko Yamamoto, Hironobu Yasui, Shingo Matsumoto, Igor A. Kirilyuk, Valery V. Khramtsov, Osamu Inanami, Hiroshi Hirata
Summary: Extracellular acidification is a hallmark of tumor malignancy, and non-invasive mapping of extracellular pH (pHe) is essential for understanding the tumor microenvironment and monitoring tumor response to metabolism-targeting drugs. A modified electron paramagnetic resonance (EPR) system achieves higher sensitivity in mapping pHe and can be used to monitor the response of other metabolism-targeting drugs.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Akihiro Taguchi, Stephen DeVience, Benoit Driesschaert, Valery V. Khramtsov, Hiroshi Hirata
Article
Radiology, Nuclear Medicine & Medical Imaging
Slimane Tounekti, Mahdi Alizadeh, Devon Middleton, James S. Harrop, Bassem Hiba, Laura Krisa, Choukri Mekkaoui, Feroze B. Mohamed
Summary: This study proposes and demonstrates a new method combining reduced field-of-view strategy with phase segmented EPI to address geometric distortion in post-operative DTI scans of patients with metal implants. The results show that the new method outperforms traditional techniques in reducing distortion.
MAGNETIC RESONANCE IMAGING
(2024)
Article
Radiology, Nuclear Medicine & Medical Imaging
Silvia Minosse, Eliseo Picchi, Valentina Ferrazzoli, Noemi Pucci, Valerio Da Ros, Raffaella Giocondo, Roberto Floris, Francesco Garaci, Francesca Di Giuliano
Summary: The aim of this study was to investigate the variation of DCE-MRI-derived kinetic parameters in brain tumors as a function of acquisition time. The results showed that K-ep and V-e were time-dependent and required longer scan times to obtain reliable parameter values, while K-trans was time-independent and remained the same in all acquisition times, making it a reliable parameter for short acquisition times.
MAGNETIC RESONANCE IMAGING
(2024)
Article
Radiology, Nuclear Medicine & Medical Imaging
Xingmin Guan, Xinheng Zhang, Hsin-Jung Yang, Rohan Dharmakumar
Summary: This study aims to investigate why DIR-prepared dark-blood T2* weighted images have lower SNR, CNR, and diagnostic accuracy for intramyocardial hemorrhage (IMH) detection compared to non-DIR-prepared bright-blood T2* images. Through phantom and animal studies, it was confirmed that the signal loss on DIR-prepared T2* images mainly originates from spin-relaxation during the DIR preparation. Therefore, when used for IMH detection, extra attention should be paid to the SNR of DIR-prepared dark-blood T2* imaging protocols.
MAGNETIC RESONANCE IMAGING
(2024)
Article
Radiology, Nuclear Medicine & Medical Imaging
Beatriz Laureano, Hassna Irzan, Helen OReilly, Sebastian Ourselin, Neil Marlow, Andrew Melbourne
Summary: Prematurity and preterm stressors have significant effects on the development of infants, especially at earlier gestations. While neonatal care advances have reduced preterm mortality rates, disability rates continue to grow in middle-income settings. Imaging the preterm brain using MR technology has improved our understanding of its development and the affected regions and networks. This research aims to support interventions, improve neurodevelopment, and provide accurate prognoses for preterm infants. This study focuses on the fully developed brain of extremely preterm subjects and examines myelin-related biomarkers to assess long-term effects. The findings suggest altered connectivity and cognitive outcomes in the adult preterm brain.
MAGNETIC RESONANCE IMAGING
(2024)
Article
Radiology, Nuclear Medicine & Medical Imaging
Julian Rauch, Frederik B. Laun, Peter Bachert, Mark E. Ladd, Tristan A. Kuder
Summary: This study presents a method for reducing concomitant field effects in double diffusion encoding (DDE) sequences by adding oscillating gradient pulses. The modified sequences successfully reduced accumulated concomitant phase without significant changes in the original sequence characteristics. The proposed method led to an increase in signal-to-noise ratio (SNR) for phantom and in vivo experiments, supported by simulations.
MAGNETIC RESONANCE IMAGING
(2024)
Article
Radiology, Nuclear Medicine & Medical Imaging
Marlon Bran Lorenzana, Shekhar S. Chandra, Feng Liu
Summary: Sparse reconstruction is important in MRI for reducing acquisition time and improving spatial-temporal resolution. This paper introduces two decoupling techniques for explicit 1D regularization and a combined 1D + 2D reconstruction technique that improves image quality.
MAGNETIC RESONANCE IMAGING
(2024)
Article
Radiology, Nuclear Medicine & Medical Imaging
Yifan Gou, W. Christopher Golden, Zixuan Lin, Jennifer Shepard, Aylin Tekes, Zhiyi Hu, Xin Li, Kumiko Oishi, Marilyn Albert, Hanzhang Lu, Peiying Liu, Dengrong Jiang
Summary: ARTS algorithm improves the reliability of Y-v estimation in noncompliant subjects, enhancing the utility of Y-v as a biomarker for brain diseases.
MAGNETIC RESONANCE IMAGING
(2024)