期刊
APPLIED SURFACE SCIENCE
卷 353, 期 -, 页码 1310-1319出版社
ELSEVIER
DOI: 10.1016/j.apsusc.2015.07.004
关键词
Biosensor; Nylon membrane; Polymer coating; Surface properties; Protein immobilization
类别
资金
- University of Malaya Research Grant [UMRG: RP009E-13AET]
- University of Malaya High Impact Research Grant UM-MOHE from the Ministry of Higher Education Malaysia [UM.C/625/1/HIR/MOHE/05]
In this paper we report the polymer-coated microporous nylon membranes and their application as platforms for protein immobilization and subsequent detection of the dengue virus (DV) in blood serum. Protein recognition experiments were performed with enzyme-linked immunosorbent assay (ELISA). The polymers used for coatings were synthesized by free-radical polymerization reaction between methyl methacrylate (MMA) and methacrylic acid (MAA) in different concentrations. The MAA monomer has carefully been chosen to generate polymers with pendant carboxyl (-COOH) groups, which also exist on polymer surfaces. A high degree of control over surface-exposed -COOH groups has been achieved through variation of monomers concentration in polymerization reaction. The general aspect of this work relies on the dengue antibody (Ab) immobilization on surface -COOH groups via physical attachment or covalent immobilization. Prior to Ab immobilization and ELISA experiment, polymer-coated nylon samples were analyzed in detail for their physical properties by atomic force microscopy (AFM), scanning electron microscopy (SEM), and water-in-air contact angle (WCA) measurements. Membranes were further analyzed by Fourier transform infrared spectroscopy (FTIR) in order to establish the relationship between wettability, porosity, and surface roughness with chemical composition and concentration of -COOH groups on the coating's surface. Optimized coatings have shown high sensitivity towards dengue Ab molecules, revealing fundamental aspect of polymer-protein interfaces as a function of surface -COOH groups' concentration. (C) 2015 Elsevier B.V. All rights reserved.
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