期刊
MACROMOLECULAR CHEMISTRY AND PHYSICS
卷 211, 期 2, 页码 136-144出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/macp.200900539
关键词
biological applications of polymers; high-throughput screening; proteins; structure; synthesis
资金
- Max-Planck Gesellschaft
- Technical University of Dortmund
- Fonds der Chemischen Industrie
- Zentrum fur Angewandte Chemische Genomik
Substantial progress in biochip technologies has established an efficient and reliable platform for advanced biological and biomedical applications. In particular, the use of protein biochips in high-throughput screens provides high content information. We briefly introduce here recent developments in protein biochip preparation with a special focus on our own work on new methods for protein immobilization and protein microarray fabrication, including the application of the Diels-Alder reaction, Staudinger ligation, 'click' sulfonamide formation, and the photochemical thiol-ene reaction. These chemical methods allow for oriented, site-specific protein conjugation on solid surfaces with high sensitivity and specificity under mild, aqueous conditions.
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