Review
Immunology
Kristian Daniel Ralph Roth, Esther Veronika Wenzel, Maximilian Ruschig, Stephan Steinke, Nora Langreder, Philip Alexander Heine, Kai-Thomas Schneider, Rico Ballmann, Viola Fuehner, Philipp Kuhn, Thomas Schirrmann, Andre Frenzel, Stefan Duebel, Maren Schubert, Gustavo Marcal Schmidt Garcia Moreira, Federico Bertoglio, Giulio Russo, Michael Hust
Summary: Antibodies are crucial for the diagnosis and treatment of diseases caused by pathogens. Their importance has been highlighted during the COVID-19 pandemic, and new technologies like antibody phage display have advanced the discovery of human monoclonal antibodies for therapeutic purposes. These technologies have expanded the possibilities for generating antibodies against a wide range of pathogens and toxins.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biology
Houssam-Eddin Khalaf, Hassan Al-Bouqaee, Manal Hwijeh, Abdul Qader Abbady
Summary: This study developed a T7 promoter plasmid for highly expressing six different Nbs and successfully immunized rabbits. The affinity-purified rabbit anti-Nb rIgG was able to detect immobilized or antigen-bound Nbs and showed comparable performance to a commercial anti-6x His antibody. The rabbit anti-Nb is a promising tool for the recombinant Nb technology.
OPEN LIFE SCIENCES
(2022)
Article
Biophysics
Olga I. Guliy, Stella S. Evstigneeva, Lev A. Dykman
Summary: Antibody phage display is a useful technique for producing antibodies to specific antigens, replacing the traditional hybrid-oma technology. It simplifies the process by manipulating DNA and bacteria, shortening the time and reducing the cost of antibody production. Antibodies prepared through phage display can be used as selective receptors in biosensors.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Food Science & Technology
Eduardo Garcia-Calvo, Aina Garcia-Garcia, Raquel Madrid, Rosario Martin, Teresa Garcia
Summary: This article reviews the development of gluten immune detection systems, which mainly detect gluten through methods such as polyclonal antibodies, monoclonal antibodies, and recombinant antibodies. Current research efforts focus not only on developing more specific and sensitive systems for gluten, but also on detecting protein motifs related to pathogenicity. New tools based on recombinant antibodies will provide adequate safety and traceability methodologies to meet the increasing market demand for gluten-free products.
Article
Multidisciplinary Sciences
Merve Arslan, Murat Karadag, Ebru Onal, Emine Gelinci, Gulcin Cakan-Akdogan, Sibel Kalyoncu
Summary: Single chain antibody fragments (scFvs) are widely used in diagnostics and therapeutics due to their small size and various engineering approaches. The design of the linker between the variable heavy and light chains of scFv plays a crucial role in its construction. The flexibility of the linker is determined by its length and sequence content, and flexible linkers such as glycine-serine (GS) are commonly preferred. However, repeated sequences in GS linkers can cause problems in PCR-based engineering and immunogenicity.
SCIENTIFIC REPORTS
(2022)
Article
Food Science & Technology
Hugo Valencia-Martinez, Timoteo Olamendi-Portugal, Rita Restano-Cassulini, Hugo Serrano-Posada, Fernando Zamudio, Lourival D. Possani, Lidia Riano-Umbarila, Baltazar Becerril
Summary: Centruroides huichol scorpion venom is lethal to mammals. In this study, four lethal toxins were characterized and a scFv HV was developed with improved affinity to Chui5 toxin. The scFv HV showed promising results in a rescue trial, protecting mice from lethal doses of venom. This research contributes to the development of a recombinant anti-venom against scorpion stings in Mexico.
Article
Agriculture, Multidisciplinary
Jin-xing Song, Meng-xiang Wang, Yi-xuan Zhang, Bo Wan, Yong-kun Du, Guo-qing Zhuang, Zi-bin Li, Song-lin Qiao, Rui Geng, Ya-nan Wu, Gai-ping Zhang
Summary: This study generated an ASFV antibody phage-display library, screened a specific single-chain antibody against ASFV major capsid protein, and identified a conserved epitope peptide that can promote the maturation of immune cells and be effectively uptaken by dendritic cells.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Pharmacology & Pharmacy
Yuki Kiguchi, Izumi Morita, Akari Tsuruno, Norihiro Kobayashi
Summary: This study successfully discovered improved high-affinity antibody mutants using a modified clonal array profiling method, which showed higher sensitivity and selectivity for cortisol compared to previous antibodies.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2022)
Article
Multidisciplinary Sciences
Yunjin Song, Hoibin Jeong, Song-Rae Kim, Yiseul Ryu, Jonghwi Baek, Jinhak Kwon, Hyeongjun Cho, Kil-Nam Kim, Joong-jae Lee
Summary: The study investigates the influence of protein binding affinity on tumor localization, highlighting the importance of optimal affinity for deep penetration into tumors. The debate over establishing a relationship between binding affinity and tumor localization still exists.
Article
Biochemical Research Methods
Ryan Kilgore, Wenning Chu, Dipendra Bhandari, David Fischler, Ruben G. Carbonell, Michael Crapanzano, Stefano Menegatti
Summary: We discovered peptide ligands that target different regions of human Fab and enable product release under mild conditions, with excellent binding capacity and dissociation constant. The selected peptide ligand showed comparable yield and purity to commercial Protein L resins in the purification of polyclonal and monoclonal Fabs.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Multidisciplinary Sciences
Patrycja Sroga, Angela Sloan, Bryce M. Warner, Kevin Tierney, Jocelyne Lew, Guodong Liu, Michael Chan, Yvon Deschambault, Derek R. Stein, Geoff Soule, Logan Banadyga, Darryl Falzarano, David Safronetz
Summary: The study demonstrates the potential of utilizing unique camelid-based IgG antibodies for prevention and treatment of deadly viral pathogens, showing reduction in viral burderns and organ-specific pathology associated with lethal diseases like HPS. Further pre-clinical evaluation is warranted due to the promising results.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Chao-Yang Huang, Ying-Yung Lok, Chia-Hui Lin, Szu-Liang Lai, Yen-Yu Wu, Chih-Yung Hu, Chu-Bin Liao, Chen-Hsuan Ho, Yu-Ping Chou, Yi-Hsuan Hsu, Yu-Hsun Lo, Edward Chern
Summary: In this study, a large and highly diverse synthetic human scFv antibody library was constructed, and novel TIM-3-neutralizing antibodies with immunomodulatory functions were identified. These findings demonstrate the potential of this library for biomedical research and the therapeutic potential of the three fully human TIM-3-neutralizing antibodies.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Medicinal
Alyssa E. Witeof, Austin L. Daniels, Laura T. Rea, Sanli Movafaghi, Katherine Kurtz, Madison Davis, Raymond W. Eveland, Christopher P. Calderon, Theodore W. Randolph
Summary: Therapeutic proteins are prone to aggregation during shipping, mainly due to the influence of low g-force stresses. Comparing populations of aggregates formed during live shipping and accelerated stability studies can provide insights into the mechanism of shipping-induced aggregation.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Chemistry, Analytical
Hiroyuki Oyama, Yuki Kiguchi, Izumi Morita, Takayuki Miyashita, Akiyoshi Ichimura, Hiroko Miyaoka, Aki Izumi, Sayaka Terasawa, Natsumi Osumi, Hiroki Tanaka, Toshifumi Niwa, Norihiro Kobayashi
Summary: The study evaluated the utility of NanoLuc luciferase (NLuc) as a fusion partner with antibody single-chain Fv fragments (scFvs), showing that NLuc fusion has higher sensitivity and specificity in ELISAs, suitable for various types of immunoassays to facilitate higher sensitivities.
ANALYTICA CHIMICA ACTA
(2021)
Article
Engineering, Chemical
Wenning Chu, Sobhana A. Sripada, Hannah R. Reese, Dipendra Bhandari, Augustus Adams, Jae Sly, Michael Crapanzano, Stefano Menegatti
Summary: This study successfully purified IgG from human plasma using a two-column process integrating LigaGuard (TM) and LigaTrap (TM) adsorbents, demonstrating the potential of a fully chromatographic process in this area.
Article
Biology
M. Frank Erasmus, Sara D'Angelo, Fortunato Ferrara, Leslie Naranjo, Andre A. Teixeira, Rebecca Buonpane, Shaun M. Stewart, Horacio G. Nastri, Andrew R. M. Bradbury
Summary: The diversity of antibody complementarity determining regions is crucial for selecting high affinity leads, and increasing the number of donors is not the only way to enhance library diversity. This study demonstrates that lymphocyte numbers from a single healthy individual can be used to construct a diverse and functional antibody library.
COMMUNICATIONS BIOLOGY
(2021)
Article
Medicine, General & Internal
Andreas Barratt-Due, Inge Christoffer Olsen, Katerina Nezvalova-Henriksen, Trine Kasine, Fridtjof Lund-Johansen, Hedda Hoel, Aleksander Rygh Holten, Anders Tveita, Alexander Mathiessen, Mette Haugli, Ragnhild Eiken, Anders Benjamin Kildal, Ase Berg, Asgeir Johannessen, Lars Heggelund, Tuva Borresdatter Dahl, Karoline Hansen Skara, Pawel Mielnik, Lan Ai Kieu Le, Lars Thoresen, Gernot Ernst, Dag Arne Lihaug Hoff, Hilde Skudal, Bard Reiakvam Kittang, Roy Bjorkholt Olsen, Birgitte Tholin, Carl Magnus Ystrom, Nina Vibeche Skei, Trung Tran, Susanne Dudman, Jan Terje Andersen, Raisa Hannula, Olav Dalgard, Ane-Kristine Finbraten, Kristian Tonby, Bjorn Blomberg, Saad Aballi, Cathrine Fladeby, Anne Steffensen, Fredrik Muller, Anne Ma Dyrhol-Riise, Marius Troseid, Pal Aukrust
Summary: A randomized controlled trial conducted in 23 hospitals in Norway found that remdesivir and hydroxychloroquine did not affect viral clearance in hospitalized COVID-19 patients. There was also no impact on mortality, degree of respiratory failure, or inflammatory variables.
ANNALS OF INTERNAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Chiara Della Bella, Maria Felicia Soluri, Simone Puccio, Marisa Benagiano, Alessia Grassi, Jacopo Bitetti, Fabio Cianchi, Daniele Sblattero, Clelia Peano, Mario Milco D'Elios
Summary: The study showed that CagY drives both B cell proliferation and T cell activation in gastric MALT lymphomas.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Editorial Material
Biochemical Research Methods
Fridtjof Lund-Johansen, Trung Tran, Adi Mehta
Summary: Co-fractionation mass spectrometry has the potential to measure numerous protein complexes in a single experiment. A meta-analysis of CF-MS data can identify a core interactome and provide a tool for aligning new results with published data.
Article
Multidisciplinary Sciences
Fortunato Ferrara, M. Frank Erasmus, Sara D'Angelo, Camila Leal-Lopes, Andre A. Teixeira, Alok Choudhary, William Honnen, David Calianese, Deli Huang, Linghan Peng, James E. Voss, David Nemazee, Dennis R. Burton, Abraham Pinter, Andrew R. M. Bradbury
Summary: This study demonstrates that highly potent human neutralizing antibodies against the SARS-CoV-2 spike protein can be generated directly from a semisynthetic naive antibody library. These antibodies have comparable affinities, developability properties, and neutralization activities to those from immune sources, providing a potential alternative to traditional immunization methods.
NATURE COMMUNICATIONS
(2022)
Correction
Multidisciplinary Sciences
Fortunato Ferrara, M. Frank Erasmus, Sara D'Angelo, Camila Leal-Lopes, Andre A. Teixeira, Alok Choudhary, William Honnen, David Calianese, Deli Huang, Linghan Peng, James E. Voss, David Nemazee, Dennis R. Burton, Abraham Pinter, Andrew R. M. Bradbury
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Hassen Kared, Asia-Sophia Wolf, Amin Alirezaylavasani, Anthony Ravussin, Guri Solum, Trung The Tran, Fridtjof Lund-Johansen, John Torgils Vaage, Lise Sofie Nissen-Meyer, Unni C. Nygaard, Olav Hungnes, Anna H. Robertson, Lisbeth Meyer Naess, Lill Trogstad, Per Magnus, Ludvig A. Munthe, Siri Mjaaland
Summary: The Omicron variant of SARS-CoV-2 has multiple mutations in the Spike protein, leading to increased transmissibility and evasion of antibodies. This study shows that vaccinated individuals who are infected with Omicron have robust immune responses, including both cellular and humoral immunity, as well as a new T cell response to non-Spike antigens.
NATURE COMMUNICATIONS
(2022)
Article
Medicine, Research & Experimental
Andre A. R. Teixeira, Sara D'Angelo, M. Frank Erasmus, Camila Leal-Lopes, Fortunato Ferrara, Laura P. Spector, Leslie Naranjo, Esteban Molina, Tamara Max, Ashley DeAguero, Katherine Perea, Shaun Stewart, Rebecca A. Buonpane, Horacio G. Nastri, Andrew R. M. Bradbury
Summary: This article presents a sequence agnostic method to improve antibody affinities while simultaneously eliminating sequence liabilities and retaining the same epitope binding as the parental antibody.
Article
Medicine, Research & Experimental
Fortunato Ferrara, Sara D'Angelo, M. Frank Erasmus, Andre A. Teixeira, Camila Leal-Lopes, Laura P. Spector, Tom Pohl, Adeline Fanni, Simon Cocklin, Andrew R. M. Bradbury
Summary: The COVID-19 pandemic has given researchers a unique opportunity to study the SARS-CoV-2 virus using novel tools. Comparative studies have implications beyond SARS-CoV-2, particularly in the areas of vaccines, host humoral immunity analysis, and antibody discovery. Additionally, potential synergies between these areas are discussed.
Article
Gastroenterology & Hepatology
Jostein H. Ibsen, Adity Chopra, Eline Benno Vaage, John T. Vaage, Fridtjof Lund-Johansen, Knut E. A. Lundin
Summary: This study investigated the humoral response to SARS-CoV-2 vaccines in patients with celiac disease (CeD) and compared it with healthy controls. The results showed that the humoral response in CeD patients is similar to that observed in healthy controls.
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY
(2023)
Article
Multidisciplinary Sciences
Nileena Velappan, Fortunato Ferrara, Sara R. D'Angelo, Devin C. Close, Leslie B. Naranjo, Madeline R. K. Bolding, Sarah C. Mozden, Camille B. Troup, Donna K. McCullough, Analyssa Gomez, Marijo Kedge, Andrew R. M. Bradbury
Summary: Antibodies play a crucial role in research, diagnostics, and therapeutics. A high throughput method based on yeast display is described to select antibodies suitable for conversion to fluorescent chimeras. This method allows for the direct identification of functional antibodies in the single chain TGP format, which exhibit higher protein expression, easier purification, and one-step binding assays.
Article
Medicine, General & Internal
Markus Hovd, Anders Asberg, Ludvig A. Munthe, Kristian Heldal, Anna V. Reisaeter, John T. Vaage, Fridtjof Lund-Johansen, Karsten Midtvedt
Summary: Kidney transplant recipients (KTRs) had reduced response to SARS-CoV-2 vaccine and increased risk of severe COVID-19. The level of vaccine-induced anti-RBD IgG did not correlate with protection from infection but was associated with reduced risk of COVID-19 death.
Article
Biochemistry & Molecular Biology
Manuel Lisetto, Mariagiulia Fattorini, Andrea Lanza, Marco Gerdol, Martin Griffin, Zhuo Wang, Fortunato Ferrara, Daniele Sblattero
Summary: Transglutaminase 2 (TG2) is a multifunctional protein involved in various physiological and pathological processes. Its role in these processes is controversial due to different effects depending on localization, cell type, or experimental conditions. This study characterized the enzymatic and functional properties of TG2 proteins in zebrafish and demonstrated their similarities to the human ortholog.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Camila Leal-Lopes, Sara 'Angelo, M. Frank Erasmus, Andre A. R. Teixeira, Graham Temples, Jinxiang Zhou, Andrew R. M. Bradbury, Fortunato Ferrara
Summary: The therapeutic use of monoclonal antibodies (mAbs) is diverse, ranging from cancer treatment to immune-mediated conditions, covering infectious and cardiovascular disorders, among others. This work describes a new high-throughput mAb expression and purification method for rapid validation of potential mAb leads, enabling efficient functional validation.
Article
Medicine, Research & Experimental
Andre Azevedo Reis Teixeira, Michael Frank Erasmus, Sara D'Angelo, Leslie Naranjo, Fortunato Ferrara, Camila Leal-Lopes, Oliver Durrant, Cecile Galmiche, Aleardo Morelli, Anthony Scott-Tucker, Andrew Raymon Morton Bradbury
Summary: This paper introduces a new antibody library format that generates high-affinity binders with drug-like developability properties directly from initial selections, reducing the need for further engineering or affinity maturation. This innovative design is expected to accelerate drug development processes by reducing the failure rate of leads due to poor antibody affinities and developability.