Production of bispecific antibodies in “knobs-into-holes” using a cell-free expression system

Production of Site-Specific Antibody–Drug Conjugates Using Optimized Non-Natural Amino Acids in a Cell-Free Expression System

(2014) Erik S. Zimmerman et al.   BIOCONJUGATE CHEMISTRY

Antitumor Efficacy of a Bispecific Antibody That Targets HER2 and Activates T Cells

(2014) T. T. Junttila et al.   CANCER RESEARCH

Structural design of disialoganglioside GD2 and CD3-bispecific antibodies to redirect T cells for tumor therapy

(2014) Ming Cheng et al.   INTERNATIONAL JOURNAL OF CANCER

Antiparallel Conformation of Knob and Hole Aglycosylated Half-Antibody Homodimers Is Mediated by a CH2–CH3 Hydrophobic Interaction

(2014) J.Michael Elliott et al.   JOURNAL OF MOLECULAR BIOLOGY

Engineering toward a bacterial “endoplasmic reticulum” for the rapid expression of immunoglobulin proteins

(2014) Dan Groff et al.   mAbs

Generation of bispecific IgG antibodies by structure-based design of an orthogonal Fab interface

(2014) Steven M Lewis et al.   NATURE BIOTECHNOLOGY

Nanobody-based products as research and diagnostic tools

(2014) Thomas De Meyer et al.   TRENDS IN BIOTECHNOLOGY

Antitumor Immunity: Easy as 1, 2, 3 with Monoclonal Bispecific Trifunctional Antibodies?

(2013) J. Maher et al.   CANCER RESEARCH

Cell-free translation of peptides and proteins:from high throughput screening to clinical production

(2013) Christopher J Murray et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Targeting T cells to tumor cells using bispecific antibodies

(2013) Stanley R Frankel et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Production and stabilization of the trimeric influenza hemagglutinin stem domain for potentially broadly protective influenza vaccines

(2013) Y. Lu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Efficient generation of stable bispecific IgG1 by controlled Fab-arm exchange

(2013) A. F. Labrijn et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Domain order of a bispecific diabody dramatically enhances its antitumor activity beyond structural format conversion: the case of the hEx3 diabody

(2013) Ryutaro Asano et al.   PROTEIN ENGINEERING DESIGN & SELECTION

A tale of two specificities: bispecific antibodies for therapeutic and diagnostic applications

(2013) Hannah Byrne et al.   TRENDS IN BIOTECHNOLOGY

Advances in bispecific biotherapeutics for the treatment of cancer

(2012) Chad May et al.   BIOCHEMICAL PHARMACOLOGY

scFv Antibody: Principles and Clinical Application

(2012) Zuhaida Asra Ahmad et al.   Clinical & Developmental Immunology

Generating Bispecific Human IgG1 and IgG2 Antibodies from Any Antibody Pair

(2012) Pavel Strop et al.   JOURNAL OF MOLECULAR BIOLOGY

A novel bispecific antibody format enables simultaneous bivalent and monovalent co-engagement of distinct target antigens

(2012) Gregory L. Moore et al.   mAbs

Progress in overcoming the chain association issue in bispecific heterodimeric IgG antibodies

(2012) Christian Klein et al.   mAbs

Aglycosylated antibodies and antibody fragments produced in a scalable in vitro transcription-translation system

(2012) Gang Yin et al.   mAbs

Dual targeting strategies with bispecific antibodies

(2012) Roland Kontermann   mAbs

A “Tag-and-Modify” Approach to Site-Selective Protein Modification

(2011) Justin M. Chalker et al.   ACCOUNTS OF CHEMICAL RESEARCH

Emerging Technologies for Making Glycan-Defined Glycoproteins

(2011) Lai-Xi Wang et al.   ACS Chemical Biology

Microscale to manufacturing scale-up of cell-free cytokine production-a new approach for shortening protein production development timelines

(2011) James F. Zawada et al.   BIOTECHNOLOGY AND BIOENGINEERING

A Two-in-One Antibody against HER3 and EGFR Has Superior Inhibitory Activity Compared with Monospecific Antibodies

(2011) Gabriele Schaefer et al.   CANCER CELL

EpCAM/CD3-Bispecific T-cell Engaging Antibody MT110 Eliminates Primary Human Pancreatic Cancer Stem Cells

(2011) M. Cioffi et al.   CLINICAL CANCER RESEARCH

Isolation and characterization of IgG1 with asymmetrical Fc glycosylation

(2011) S. Ha et al.   GLYCOBIOLOGY

A prokaryote-based cell-free translation system that efficiently synthesizes glycoproteins

(2011) Cassandra Guarino et al.   GLYCOBIOLOGY

Efficient disulfide bond formation in virus-like particles

(2011) Bradley C. Bundy et al.   JOURNAL OF BIOTECHNOLOGY

Immunoglobulin domain crossover as a generic approach for the production of bispecific IgG antibodies

(2011) W. Schaefer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Stable IgG-like Bispecific Antibodies Directed toward the Type I Insulin-like Growth Factor Receptor Demonstrate Enhanced Ligand Blockade and Anti-tumor Activity

(2010) Jianying Dong et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Development of a Two-part Strategy to Identify a Therapeutic Human Bispecific Antibody That Inhibits IgE Receptor Signaling

(2010) Janet Jackman et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Enhancing Antibody Fc Heterodimer Formation through Electrostatic Steering Effects

(2010) Kannan Gunasekaran et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Effector Cell Recruitment with Novel Fv-based Dual-affinity Re-targeting Protein Leads to Potent Tumor Cytolysis and in Vivo B-cell Depletion

(2010) Syd Johnson et al.   JOURNAL OF MOLECULAR BIOLOGY

Chemical generation of bispecific antibodies

(2010) V. R. Doppalapudi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Introducing antigen-binding sites in structural loops of immunoglobulin constant domains: Fc fragments with engineered HER2/neu-binding sites and antibody properties

(2010) G. Wozniak-Knopp et al.   PROTEIN ENGINEERING DESIGN & SELECTION

SEEDbodies: fusion proteins based on strand-exchange engineered domain (SEED) CH3 heterodimers in an Fc analogue platform for asymmetric binders or immunofusions and bispecific antibodies†

(2010) Jonathan H. Davis et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Strategies to Extend Plasma Half-Lives of Recombinant Antibodies

(2009) Roland E. Kontermann   BIODRUGS

Glycosylation as a strategy to improve antibody-based therapeutics

(2009) Roy Jefferis   NATURE REVIEWS DRUG DISCOVERY

A modular IgG-scFv bispecific antibody topology

(2009) Kelly Davis Orcutt et al.   PROTEIN ENGINEERING DESIGN & SELECTION

High yield cell-free production of integral membrane proteins without refolding or detergents

(2008) Jessica J. Wuu et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Glycoprotein Synthesis: An Update

(2008) David P. Gamblin et al.   CHEMICAL REVIEWS

Aglycosylated immunoglobulin G1 variants productively engage activating Fc receptors

(2008) S. L. Sazinsky et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA