Article
Immunology
Ahmad Najafi, Reza Valadan, Hossein Asgarian-Omran, Alireza Rafiei, Mohsen Tehrani
Summary: In this study, a recombinant phage was generated using the phage display method to select monoclonal phages that can react with the dimerization domain of HER2 receptor. Functional tests confirmed their specificity and anti-tumor properties.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yu Jung Kim, Min Ho Lee, Se-Ra Lee, Hyo-Young Chung, Kwangmin Kim, Tae Gyu Lee, Dae Young Kim
Summary: Phage display was used to develop human monoclonal antibodies (mAbs) that neutralize SARS-CoV-2. These antibodies showed desirable neutralizing activities and properties, making them potential candidates for antibody therapeutics for treating COVID-19, as well as for diagnostics and research tools.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Ji Woong Kim, Hyun Jung Kim, Kyun Heo, Yoonwoo Lee, Hui Jeong Jang, Ho-Young Lee, Jun Won Park, Yea Bin Cho, Ji Hyun Lee, Ha Gyeong Shin, Ha Rim Yang, Hye Lim Choi, Hyun Bo Shim, Sukmook Lee
Summary: This study presents a novel dual-targeting strategy using bispecific antibodies (bsAbs) that specifically recognize both the SARS-CoV-2 receptor-binding domain (RBD) and fusion peptide (FP), crucial domains for viral attachment and fusion. The bsAb demonstrated higher neutralization efficacy against SARS-CoV-2 variants compared to the parental RBD-specific monoclonal antibody (mAb). This finding provides a potential approach for rapid development and management against evolving SARS-CoV-2 variants.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Virology
Kazuya Nagano, Yasuo Tsutsumi
Summary: Antibody drugs with high affinity and specificity are effective for intractable diseases, such as cancers and autoimmune diseases. The recent breakthrough in antibody drugs is largely due to the contribution of phage display technology.
Article
Chemistry, Analytical
Yanbo Wang, Mingyang Wang, Haipeng Yu, Ge Wang, Pengxin Ma, Shuang Pang, Yiming Jiao, Aihua Liu
Summary: PSA is an important index for prostate cancer diagnosis and treatment. In this study, a biopanning method for PSA was established using a pIII phage display library. Two phage monoclonal antibodies specific to PSA were obtained, and an ELISA system was constructed for detecting tPSA with high accuracy.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Oncology
Xuefei Bai, Wenhui Liu, Shijie Jin, Wenbin Zhao, Yingchun Xu, Zhan Zhou, Shuqing Chen, Liqiang Pan
Summary: Research on bispecific antibody formats has led to the development of a BiFab format using sortase A-mediated bio-click chemistry, showing comparable activity and potential for personalized tumor immunotherapy in the future.
Review
Biochemical Research Methods
Rui Wang, Hui-Da Li, Ying Cao, Zi-Yi Wang, Ting Yang, Jian-Hua Wang
Summary: Viruses, specifically M13 phage, are being utilized in biosensing and biomedicine due to their versatility, specific reactivities, and responsiveness to genetic modifications. M13 phages can be engineered and modified to create a multifunctional analysis platform, with different functional regions performing their tasks without interference. The unique filamentous morphology and flexibility of M13 phages enhance target affinity and signal amplification in analytical applications.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Medicine, Research & Experimental
Ernest S. Smith, Leslie A. Balch, Maria Scrivens, Shuying Shi, Wei Wang, Caroline D. Harvey, Angelica A. Cornelison, Malgorzata Gil-Moore, Renee A. Kirk, Loretta L. Mueller, Richard L. Hall, Alan P. Howell, Christine A. Reilly, Jessica M. Mayer, Francis G. Murante, Kari A. Viggiani, Elaine M. Gersz, Holm Bussler, Madeleine R. Keefe, Elizabeth E. Evans, Mark J. Paris, Maurice Zauderer
Summary: The limitation in antibody discovery against complex antigens is the lack of reproducible pure and properly folded antigen sources. A technology has been developed to incorporate membrane proteins like GPCRs directly into the membrane of poxvirus. The protein of interest is correctly folded and expressed in the viral membrane, eliminating the need for detergents or refolding for downstream use. The selective incorporation of proteins into the viral membrane makes the poxvirus antigen a cleaner target for in vitro panning. Antigen-expressing virus can be easily purified at scale and used for antibody selection using any in vitro display platform.
Article
Biochemistry & Molecular Biology
Ahmed Mohammed, Weihong Zeng, Hylemariam Mihiretie Mengist, Arnaud John Kombe Kombe, Huichao Ou, Yunru Yang, Zhao Dan, Zhihao Xu, Huan Ma, Tengchuan Jin
Summary: The study developed and characterized V-C1 domain-specific Nbs targeting human RAGE, which exhibited high thermostability and improved binding affinity after homodimerization. Competitive ELISA demonstrated their potential in blocking V-C1-hS100B, offering new insights into treating RAGE-associated diseases.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Seyedeh Zahra Bahojb Mahdavi, Fatemeh Oroojalian, Shirin Eyvazi, Maryam Hejazi, Behzad Baradaran, Nasser Pouladi, Mohammad Reza Tohidkia, Ahad Mokhtarzadeh, Serge Muyldermans
Summary: Antibodies, as therapeutic and diagnostic molecules, play a crucial role in disease treatment, especially in cancer therapy. Alternative approaches, such as display selection technologies, have been developed to overcome the limitations of traditional hybridoma technology. These methods, including phage display, bacterial display, and yeast display, enable the screening and generation of high affinity antibodies with low immunogenicity risk. Understanding the basics of each method is important for antibody design suitable for different diseases, including cancer.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Mohammad Ridhuan Mohd Ali, Jia Siang Sum, Nurul Najian Aminuddin Baki, Yee Siew Choong, Nur Asyura Nor Amdan, Fairuz Amran, Theam Soon Lim
Summary: This study aimed to develop specific monoclonal antibodies against LipL21 antigen for future use in rapid and accurate immunoassay for leptospirosis. The generated monoclonal antibody against rLipL21 will be evaluated as a detection reagent for the diagnosis of human leptospirosis in a future study.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Immunology
Desislava Yanakieva, Lena Vollmer, Andreas Evers, Vanessa Siegmund, Paul Arras, Lukas Pekar, Achim Doerner, Bernhard Valldorf, Harald Kolmar, Stefan Zielonka, Simon Krah
Summary: In this study, a novel symmetric bispecific antibody format was presented by grafting cattle-derived knob paratopes onto peripheral loops of the IgG1 Fc region. Knob architectures obtained from bovine ultralong CDR-H3 antibodies were inserted into the AB loop or EF loop of the CH3 domain to introduce an artificial binding specificity into an IgG molecule. The generated bispecific antibodies demonstrated retained binding affinities of the inserted knob domains and versatile effector cell redirection functionalities without compromising Fc mediated functions such as FcRn or Fc gamma RIIIa binding.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shuyu Huang, Aina Segues, Martin Waterfall, David Wright, Charlotte Vayssiere, Sander M. J. van Duijnhoven, Andrea van Elsas, Alice J. A. M. Sijts, Dietmar M. Zaiss
Summary: T cell engager (TCE) antibodies have shown promise as cancer therapeutics by linking cytotoxic T cells to tumor cells. This study evaluates a novel bispecific antibody format, Fab x sdAb-Fc, as a T-cell redirecting bispecific antibody (TbsAbs) and identifies the hinge design as a key factor influencing their anti-tumor activity.
Article
Chemistry, Multidisciplinary
Zhenqiang Shi, Xiancheng Zhang, Xijing Yang, Xiaoyu Zhang, Fei Ma, Hui Gan, Junjun Chen, Dongdong Wang, Wenjing Sun, Jingxia Wang, Cunli Wang, Liting Lyu, Kaiguang Yang, Lijing Deng, Guangyan Qing
Summary: A robust strategy based on phage display screening and hemocompatible peptide bottlebrush polymer design is proposed for specific clearance of targeted Lipopolysaccharide (LPS) from circulating blood. A novel peptide with high affinity, specificity, and neutralization activity against the targeted LPS is discovered. This work provides a universal paradigm for developing a highly selective hemoadsorbent library fully covering the LPS family, promising for a new era of precision medicine in sepsis therapy.
ADVANCED MATERIALS
(2023)
Article
Immunology
Sebastian Lutz, Katja Klausz, Anca-Maria Albici, Lea Ebinger, Lea Sellmer, Hannah Teipel, Andre Frenzel, Anna Langner, Dorothee Winterberg, Steffen Krohn, Michael Hust, Thomas Schirrmann, Stefan Duebel, Regina Scherliess, Andreas Humpe, Martin Gramatzki, Christian Kellner, Matthias Peipp
Summary: This study successfully activated natural killer cells and induced lysis of lymphoma cells by preparing novel bispecific antibodies. The study also found that co-administration with specific monoclonal antibodies or bispecific T cell engagers enhanced antibody-dependent cell-mediated cytotoxicity and T cell activation. Furthermore, the study proposed a combinatorial approach targeting multiple tumor antigens, which has the potential to optimize antibody cancer therapies.
FRONTIERS IN IMMUNOLOGY
(2023)