Article
Biochemistry & Molecular Biology
Hye Lim Choi, Ha Rim Yang, Ha Gyeong Shin, Kyusang Hwang, Ji Woong Kim, Ji Hyun Lee, Taehoon Ryu, Yushin Jung, Sukmook Lee
Summary: Antibody phage display is a crucial technology for discovering and developing target-specific monoclonal antibodies. This study constructed a large human combinatorial single-chain variable fragment library with high diversity, which can be used for the rapid development of recombinant human monoclonal antibodies for therapeutic and diagnostic applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Mohammad Ridhuan Mohd Ali, Jia Siang Sum, Nurul Najian Aminuddin Baki, Yee Siew Choong, Nur Asyura Nor Amdan, Fairuz Amran, Theam Soon Lim
Summary: This study aimed to develop specific monoclonal antibodies against LipL21 antigen for future use in rapid and accurate immunoassay for leptospirosis. The generated monoclonal antibody against rLipL21 will be evaluated as a detection reagent for the diagnosis of human leptospirosis in a future study.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Yu Jung Kim, Min Ho Lee, Se-Ra Lee, Hyo-Young Chung, Kwangmin Kim, Tae Gyu Lee, Dae Young Kim
Summary: Phage display was used to develop human monoclonal antibodies (mAbs) that neutralize SARS-CoV-2. These antibodies showed desirable neutralizing activities and properties, making them potential candidates for antibody therapeutics for treating COVID-19, as well as for diagnostics and research tools.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Jingru Liang, Hang Dong, Fei Xu, Baowei Li, Haimei Li, Limei Chen, Mei Li, Yingchu Liu, Guosheng Jiang, Jinhua Dong
Summary: In this study, a monoclonal antibody named ESC9 was developed, and an immunosensor called ESC9 Quenchbody (Q-body) was generated for rapid and sensitive detection of estradiol. The ESC9 Q-body showed potential for clinical applications due to its excellent detection speed and sensitivity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Frederik Peissert, Louis Pluss, Anna Maria Giudice, Tiziano Ongaro, Alessandra Villa, Abdullah Elsayed, Lisa Nadal, Sheila Dakhel Plaza, Luigi Scietti, Emanuele Puca, Roberto De Luca, Federico Forneris, Dario Neri
Summary: Programmed cell death protein 1 (PD-1) is an immunoregulatory target that can be targeted by different monoclonal antibodies for cancer therapy. In this study, researchers identified a new anti-PD-1 antibody with unique binding specificity and demonstrated its ability to block the interaction between PD-1 and PD-L1.
Article
Immunology
Chao-Yang Huang, Ying-Yung Lok, Chia-Hui Lin, Szu-Liang Lai, Yen-Yu Wu, Chih-Yung Hu, Chu-Bin Liao, Chen-Hsuan Ho, Yu-Ping Chou, Yi-Hsuan Hsu, Yu-Hsun Lo, Edward Chern
Summary: In this study, a large and highly diverse synthetic human scFv antibody library was constructed, and novel TIM-3-neutralizing antibodies with immunomodulatory functions were identified. These findings demonstrate the potential of this library for biomedical research and the therapeutic potential of the three fully human TIM-3-neutralizing antibodies.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Medicine, Research & Experimental
Ernest S. Smith, Leslie A. Balch, Maria Scrivens, Shuying Shi, Wei Wang, Caroline D. Harvey, Angelica A. Cornelison, Malgorzata Gil-Moore, Renee A. Kirk, Loretta L. Mueller, Richard L. Hall, Alan P. Howell, Christine A. Reilly, Jessica M. Mayer, Francis G. Murante, Kari A. Viggiani, Elaine M. Gersz, Holm Bussler, Madeleine R. Keefe, Elizabeth E. Evans, Mark J. Paris, Maurice Zauderer
Summary: The limitation in antibody discovery against complex antigens is the lack of reproducible pure and properly folded antigen sources. A technology has been developed to incorporate membrane proteins like GPCRs directly into the membrane of poxvirus. The protein of interest is correctly folded and expressed in the viral membrane, eliminating the need for detergents or refolding for downstream use. The selective incorporation of proteins into the viral membrane makes the poxvirus antigen a cleaner target for in vitro panning. Antigen-expressing virus can be easily purified at scale and used for antibody selection using any in vitro display platform.
Article
Biotechnology & Applied Microbiology
Qianlin Li, Fuqiang Zhang, Yi Lu, Huan Hu, Jin Wang, Cheng Guo, Qiang Deng, Conghui Liao, Qin Wu, Tingsong Hu, Zeliang Chen, Jiahai Lu
Summary: In this study, we developed potent multivalent VHH antibodies targeting the E2 glycoprotein of CHIKV, with Nb-2E8 and Nb-3C5 showing high-affinity binding within the nanomolar range.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Jin-xing Song, Meng-xiang Wang, Yi-xuan Zhang, Bo Wan, Yong-kun Du, Guo-qing Zhuang, Zi-bin Li, Song-lin Qiao, Rui Geng, Ya-nan Wu, Gai-ping Zhang
Summary: This study generated an ASFV antibody phage-display library, screened a specific single-chain antibody against ASFV major capsid protein, and identified a conserved epitope peptide that can promote the maturation of immune cells and be effectively uptaken by dendritic cells.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Biochemistry & Molecular Biology
Xuelian Bai, Moonseon Jang, Nam Ju Lee, Thi Thu Ha Nguyen, Mooyoung Jung, Jeong Yeon Hwang, Hyunbo Shim
Summary: Antibody discovery by phage display involves two phases, the binding phase and the amplification phase. The amplification efficiency of antibody fragments varies widely among different sequences, leading to diminished efficiency of antibody isolation. In this study, a novel synthetic scFv library with diversities aimed at improved amplification efficiency was designed and constructed. CDR sequences were analyzed to design the new library, and a machine learning method was used to predict potential fast-amplified sequences. The new library performed better in terms of binders isolated, unique sequences, and binder enrichment compared to the previous generation library.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Wanzhi Huang, Victoria Soeung, David M. Boragine, Liya Hu, B. V. Venkataram Prasad, Mary K. Estes, Robert L. Atmar, Timothy Palzkill
Summary: This study utilized a high-resolution method to map multiple antibody binding sites simultaneously from complex serum samples. The results indicated that a relatively small number of sites on the virus bind a large number of independently generated antibodies, suggesting that immunodominance plays a role in the humoral immune response to NoV infections.
JOURNAL OF VIROLOGY
(2021)
Review
Virology
Kazuya Nagano, Yasuo Tsutsumi
Summary: Antibody drugs with high affinity and specificity are effective for intractable diseases, such as cancers and autoimmune diseases. The recent breakthrough in antibody drugs is largely due to the contribution of phage display technology.
Article
Chemistry, Analytical
Sandra Oloketuyi, Robert Bernedo, Andreas Christmann, Justyna Borkowska, Giulia Cazzaniga, Horst Wilhelm Schuchmann, Joanna Niedziolka-Joensson, Katarzyna Szot-Karpinska, Harald Kolmar, Ario de Marco
Summary: C-reactive protein (CRP) is an inflammation biomarker that should be accurately quantified during infections and healing processes. Nanobodies show promise as alternatives to conventional antibodies in immunodiagnostics, with the ability to achieve higher binder density on capture surfaces. By comparing two different selection strategies, researchers were able to isolate anti-CRP nanobodies with low nanomolar affinity, suitable for use in ELISA and immunoprecipitation assays. One of the nanobodies was fused to SpyTag and used in an electrochemical biosensor to detect CRP with high sensitivity.
Article
Biotechnology & Applied Microbiology
Yaqi Yang, Siji Nian, Lin Li, Xue Wen, Qin Liu, Bo Zhang, Yu Lan, Qing Yuan, Yingchun Ye
Summary: Enhanced EphA2 expression is a crucial target for anti-tumor therapy, with high affinity scFvs against EphA2 successfully screened from an immune library constructed using PBMCs from cancer patients. Modified antibodies showed improved affinity and the ability to bind to EphA2, tumor cells, and tissues, inhibiting tumor growth to some extent. This study highlights the potential of immune libraries from cancer patients in screening for high-affinity antibodies with therapeutic effects.
Article
Biochemistry & Molecular Biology
Jingru Liang, Hang Dong, Hongsheng Wang, Zhengjun Yi, Guosheng Jiang, Takashi Inagaki, Celso E. Gomez-Sanchez, Jinhua Dong, Hiroshi Ueda
Summary: Aldosterone is a hormone that regulates sodium ion and water reabsorption in the kidney. In this study, the gene encoding the anti-aldosterone antibody A2E11 was cloned and analyzed, and a sensor was developed to detect aldosterone. The sensor showed a detection limit of 24.1 pg/mL with a wide detection range, and it could accurately detect aldosterone within 2 minutes. This sensor has potential applications in the diagnosis of diseases such as hypertension.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Review
Immunology
Francoise Aubrit, Fabien Perugi, Arnaud Leon, Fabienne Guehenneux, Patrick Champion-Arnaud, Mehdi Lahmar, Klaus Schwamborn
Article
Multidisciplinary Sciences
Madelon Q. Wentink, Tilman M. Hackeng, Sebastien P. Tabruyn, Wouter C. Puijk, Klaus Schwamborn, Daniele Altschuh, Rob H. Meloen, Teun Schuurman, Arjan W. Griffioen, Peter Timmerman
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Immunology
Arnaud Leon, Anne-Laure David, Brice Madeline, Laurence Guianvarc'h, Elodie Dureau, Patrick Champion-Arnaud, Matthias Hebben, Thierry Huss, Benoit Chatrenet, Klaus Schwamborn
Editorial Material
Immunology
Klaus Schwamborn
Article
Biotechnology & Applied Microbiology
Alexander Nikolay, Arnaud Leon, Klaus Schwamborn, Yvonne Genzel, Udo Reichl
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Nadine Martin, Klaus Schwamborn, Valerie Schreiber, Andreas Werner, Christelle Guillier, Xiang-Dong Zhang, Oliver Bischof, Jacob-S Seeler, Anne Dejean
Article
Biochemistry & Molecular Biology
Klaus Schwamborn, Puck Knipscheer, Evert van Dijk, Willem J. van Dijk, Titia K. Sixma, Rob H. Meloen, Johannes P. M. Langedijk
JOURNAL OF BIOCHEMISTRY
(2008)
Article
Biochemistry & Molecular Biology
Nadine Martin, Klaus Schwamborn, Henning Urlaub, Boyi Gan, Jun-Lin Guan, Anne Dejean
MOLECULAR AND CELLULAR BIOLOGY
(2008)
Article
Multidisciplinary Sciences
Daniele V. F. Tauriello, Ingrid Jordens, Katharina Kirchner, Jerry W. Slootstra, Tom Kruitwagen, Britta A. M. Bouwman, Maria Noutsou, Stefan G. D. Rudiger, Klaus Schwamborn, Alexandra Schambony, Madelon M. Maurice
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2012)
Article
Biochemical Research Methods
Fabien Perugi, Caroline Freslon-Evain, Luc Batard, Pauline Guillet, Klaus Schwamborn
Retraction
Biochemistry & Molecular Biology
Oliver Bischof, Klaus Schwamborn, Nadine Martin, Andreas Werner, Claudio Sustmann, Rudolf Grosschedl, Anne Dejean
Article
Biochemistry & Molecular Biology
Oliver Bischof, Klaus Schwamborn, Nadine Martin, Andreas Werner, Claudio Sustmann, Rudolf Grosschedl, Anne Dejean
Article
Biochemistry & Molecular Biology
VV Kravchenko, JC Mathison, K Schwamborn, F Mercurio, RJ Ulevitch
JOURNAL OF BIOLOGICAL CHEMISTRY
(2003)
Article
Immunology
R Weil, K Schwamborn, A Alcover, C Bessia, V Di Bartolo, A Israel