Article
Food Science & Technology
Xiao Hu, Chuang Pan, Miaomiao Cai, Laihao Li, Xianqing Yang, Huan Xiang, Shengjun Chen
Summary: In this study, Grateloupia livida protein was hydrolyzed by different proteases to obtain antioxidant peptides, with hydrolysates from neutral protease showing the highest activity and low molecular weight distribution. The isolated fraction F3 exhibited the highest antioxidant ability, with four identified peptides potentially suitable for use as natural antioxidants.
Article
Biochemistry & Molecular Biology
Yannan Xu, Yanxia Feng, Yulan Yang, Le Cheng, Min Zhou, Yu Guo, Difeng Ren
Summary: The study utilized alcalase and neutrase to hydrolyze defatted walnut meal protein and obtained anti-inflammatory peptides. The highest anti-inflammatory activity fraction was identified through ultrafiltration and liquid chromatography-tandem mass spectrometry (LC-MS/MS), resulting in 579 peptides. Four stable binding tripeptides were successfully identified through virtual screening. The synthetic peptide assay showed that FPL exhibited excellent anti-inflammatory activity and could be used as a natural active substance in the functional food and pharmaceutical industries.
Article
Multidisciplinary Sciences
Liu Yang, Yan Xing, Rui Chen, He Ni, Hai-Hang Li
Summary: This study aimed to extract and study the antioxidant peptides in crocodile meat. Crocodile meat proteins were extracted with water and hydrolyzed by papain, the antioxidant peptides were isolated and purified by silica gel column chromatography and TLC, and four highly active peptides were identified by LC/MS. The results indicated that the 3 kDa hydrolyzed peptides of crocodile meat had high antioxidant activity.
SCIENTIFIC REPORTS
(2022)
Article
Food Science & Technology
Xiaoyi Li, Chunsong Feng, Hui Hong, Yan Zhang, Zhigang Luo, Quanyu Wang, Yongkang Luo, Yuqing Tan
Summary: ACE inhibitory peptides were detected in whey protein hydrolysate, which may provide novel ACE inhibitors for hypertension management.
Article
Chemistry, Medicinal
Xiao Hu, Ya Zhou, Shaobo Zhou, Shengjun Chen, Yanyan Wu, Laihao Li, Xianqing Yang
Summary: The study investigated the XO inhibitory effects of peptides purified from round scad hydrolysates, revealing FPSV and FPFP peptides to exhibit strong inhibitory activity. Fluorescence quenching mechanism and inhibition kinetics indicated their reversible and mixed-type inhibition. Molecular docking highlighted the importance of π-π stacking in the XO inhibitory activity of the peptides.
Article
Food Science & Technology
Jingxin Gao, Tiange Li, Duoduo Chen, Haofeng Gu, Xueying Mao
Summary: This study demonstrated the antioxidant activities of hemp seed protein hydrolysates and identified two potential antioxidant peptides, showing that they could block specific enzyme active cavities to exert antioxidant effects. Molecular docking analysis revealed the potential mechanisms of action for these peptides.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Rong Zan, Ling Zhu, Gangcheng Wu, Hui Zhang
Summary: Chickpea protein hydrolysates (CPHs) were found to activate alcohol dehydrogenase (ADH). Four novel peptides from CPHs were identified to have ADH activation ability. The highest ADH activating ability was observed in CPHs obtained by hydrolysis with Alcalase for 30 min (CPHs-Pro-30), and the activation rate remained over 80% after simulated gastrointestinal digestion.
Article
Chemistry, Multidisciplinary
Jiao Li, Jilei Su, Min Chen, Jiao Chen, Wenping Ding, Yanqun Li, Hao Yin
Summary: This study aimed to isolate potent antihypertensive peptides from Pinctada fucata through in silico analysis. The potential ACEI peptide sequences were obtained and identified from fractions C17 and C18 by LC-ESI-MS/MS, leading to the discovery of two novel tetrapeptides, FRVW and LPYY, for synthesis. In vitro ACEI tests showed that the peptides had promising IC50 values, with FRVW acting as a noncompetitive inhibitor and LPYY as a mixed-mode inhibitor, indicating their potential as antihypertensive agents.
Article
Agriculture, Multidisciplinary
Rong Zan, Qiming Wu, Yiling Chen, Gangcheng Wu, Hui Zhang, Ling Zhu
Summary: This study found that chickpea protein hydrolysates (CPHs) obtained through Neutrase hydrolysis had high DPP-IV inhibitory (DPP-IVi) activity. Four peptides with strong DPP-IVi activity were identified through peptide sequence analysis and molecular docking, among which IAIPPGIPYW showed the strongest activity. Chickpea could be used as a natural source of hypoglycemic peptides.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Applied
Lee Suan Chua, Farah Izana Abdullah, Teck Kwang Lim, Qingsong Lin
Summary: This study aimed to extract bioactive peptides from the white and purple flower varieties of Orthosiphon aristatus leaves. The results showed that trypsin hydrolysates had higher antioxidant and antibacterial activities. The white flower hydrolysates exhibited higher radical scavenging activity, while the purple flower hydrolysates showed higher ferric reducing power and bacterial growth inhibition.
Article
Food Science & Technology
Fu Tian, Sureelak Rodtong, Kanjana Thumanu, Yanling Hua, Sittiruk Roytrakul, Jirawat Yongsawatdigul
Summary: Due to the overuse and abuse of antibiotics, antibiotic resistant bacteria have emerged. Antimicrobial peptides (AMPs) are gaining attention as alternative antimicrobial agents due to their unique mode of action that hinders bacterial resistance. This study isolated and identified two novel antibacterial peptides, VSDH and CCCPKAF, from Alcalase-hydrolyzed chicken plasma, which showed effective antibacterial activity against Bacillus cereus DMST 5040 with different mechanisms of action. VSDH targeted intracellular molecules, while CCCPKAF caused cell membrane disintegration. Molecular docking analysis revealed that VSDH had high binding affinity for enzymes involved in DNA synthesis and a chaperone protein, leading to the misfolding of intracellular proteins. NMR and molecular dynamics simulations suggested that VSDH chelated with Mg2+, contributing to its antibacterial activity.
Article
Multidisciplinary Sciences
Hui-Ju Chen, Fan-Jhen Dai, Cheng-You Chen, Siao-Ling Fan, Ji-Hong Zheng, Chi-Fai Chau, Yung-Sheng Lin, Chin-Shuh Chen
Summary: Rice protein was enzymatically hydrolyzed to produce rice protein hydrolysates (RPH), which were further fractionated using ultrafiltration membrane (UF) based on molecular weight. Peptides with molecular weight less than 1 kDa exhibited superior antioxidant properties. UF was effective in selectively separating antioxidant peptides. Pearson correlation analysis showed a strong positive correlation between total phenolic concentration and oxygen radical absorbance capacity (ORAC). Amino acid contents were negatively correlated with the scavenging activity of certain radicals. Reducing power was associated with aromatic amino acid contents. Enzymatic hydrolysis proved to be an effective method for extracting and isolating natural antioxidant proteins from broken rice, preserving their nutritional quality and facilitating future applications.
SCIENTIFIC REPORTS
(2023)
Article
Agriculture, Multidisciplinary
Madison Fomich, Vermont P. Dia, Uvinduni I. Premadasa, Benjamin Doughty, Hari B. Krishnan, Tong Wang
Summary: The study investigated the ice recrystallization inhibition (IRI) activity of soy protein isolate (SPI) hydrolysates and determined that the F1 fraction (4-14 kDa) had the highest IRI activity. Vibrationalsum frequency generation (VSFG) analysis indicated that the molecular interactions at the water/air interface were linked to IRI activity.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Chemistry, Multidisciplinary
Rhessa Grace Guanga Ortizo, Vishal Sharma, Mei-Ling Tsai, Jia-Xiang Wang, Pei-Pei Sun, Parushi Nargotra, Chia-Hung Kuo, Chiu-Wen Chen, Cheng-Di Dong
Summary: Bioactive peptides derived from fish byproduct protein hydrolysate have vast potential as functional food ingredients. Enzymatic hydrolysis is the preferred method for the preparation of these peptides due to its high specificity, mild reaction conditions, and lack of residual organic solvents. However, the use of proteases and the availability of food-grade enzymes pose limitations. Current research focuses on the purification and identification of these peptides, as well as their bioactivities, including antiproliferative, antimicrobial, antihypertensive, antiglycemic, antitumor, and antioxidative functions. The growing interest in proteomics and the utilization of marine and aquatic waste stems from the bioactivity and sustainability of these products.
APPLIED SCIENCES-BASEL
(2023)
Article
Chemistry, Applied
Sophie Beaubier, Erwann Durand, Charles Lenclume, Frederic Fine, Arnaud Aymes, Xavier Framboisier, Romain Kapel, Pierre Villeneuve
Summary: An optimized proteolysis process was used to obtain metal-chelating peptides from rapeseed meal proteins. IMAC was applied to identify the structure of the most active peptides, which were found to be small peptides composed of 2 to 20 amino acids. These peptides showed significant chelating efficiency and performed well as antioxidants in emulsion.
Article
Chemistry, Applied
Remi Przybylski, Laurent Bazinet, Loubna Firdaous, Mostafa Kouach, Jean-Francois Goossens, Pascal Dhulster, Naima Nedjar
Article
Biochemistry & Molecular Biology
Latifa Chahed, Rafik Balti, Sawsen Elhiss, Nadia Bouchemal, Nadine Ajzenberg, Veronique Ollivier, Frederic Chaubet, Raoui Mounir Maaroufi, Mohamed Ben Mansour
PROCESS BIOCHEMISTRY
(2020)
Article
Food Science & Technology
Rafik Balti, Mohamed Ben Mansour, Nourhene Zayoud, Romain Le Balc'h, Nicolas Brodu, Abdellah Arhaliass, Anthony Masse
Article
Microbiology
Hannah Luise Bruck, Francois Coutte, Pascal Dhulster, Sebastien Gofflot, Philippe Jacques, Frank Delvigne
Article
Food Science & Technology
Cyril Raveschot, Francois Coutte, Marc Fremont, Maxime Vaeremans, Jamyan Dugersuren, Shirchin Demberel, Djamel Drider, Pascal Dhulster, Christophe Flahaut, Benoit Cudennec
FOOD RESEARCH INTERNATIONAL
(2020)
Article
Biochemistry & Molecular Biology
Remi Przybylski, Laurent Bazinet, Loubna Firdaous, Mostafa Kouach, Jean-Francois Goossens, Pascal Dhulster, Naima Nedjar-Arroume
Article
Environmental Sciences
Takwa Wakkaf, Radhouan El Zrelli, Mikael Kedzierski, Rafik Balti, Moez Shaiek, Lamjed Mansour, Sabiha Tlig-Zouari, Stephane Bruzaud, Lotfi Rabaoui
MARINE POLLUTION BULLETIN
(2020)
Article
Biochemistry & Molecular Biology
Mira Abou-Diab, Jacinthe Thibodeau, Barbara Deracinois, Christophe Flahaut, Ismail Fliss, Pascal Dhulster, Naima Nedjar, Laurent Bazinet
Article
Biochemistry & Molecular Biology
Mira Abou-Diab, Jacinthe Thibodeau, Barbara Deracinois, Christophe Flahaut, Ismail Fliss, Pascal Dhulster, Laurent Bazinet, Naima Nedjar
Article
Biotechnology & Applied Microbiology
Ali Mezni, Abdelhafidh Khazri, Hassen Khazri, Amine Mezni, Aziza Maalaoui, Samir Touyalia, Rafik Balti, Mohamed Dellali, Ezzeddine Mahmoudi, Hamouda Beyrem
Summary: The Au-TiO2 NPs have the potential to interact with the biochemical status of rats and cause undesirable effects, such as oxidative stress and neurotoxicity. The study highlights the impact of using Au-TiO2 NPs in the medical field for further exploration.
Article
Biochemistry & Molecular Biology
Sophie Beaubier, Remi Przybylski, Alice Bodin, Naima Nedjar, Pascal Dhulster, Romain Kapel
Summary: This study investigated the use of a simulation method to predict the yield and enrichment of NKT during ultrafiltration of bovine hemoglobin hydrolysates. The results showed that the 3% DH colored hydrolysate was the most promising in terms of NKT enrichment, with a maximal enrichment factor of about 29 and NKT purity of 70% in the permeate. This reliable method for predicting ultrafiltration performances to enrich peptides of interest is part of a global approach to valorize protein resources from various by-products.
Article
Engineering, Chemical
Rafik Balti, Nourhene Zayoud, Florence Hubert, Lucie Beaulieu, Anthony Masse
Summary: Membrane diafiltration process was used to fractionate water soluble proteins from Spirulina, resulting in high purity phycocyanin extracts and production of blue color permeates with low chlorophyll content. High recovery rates and protein concentration were achieved, with successful size-based fractionation of proteins.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Review
Energy & Fuels
Cedric Decarpigny, Abdulhadi Aljawish, Cedric His, Bertrand Fertin, Muriel Bigan, Pascal Dhulster, Michel Millares, Renato Froidevaux
Summary: The use of renewable energy is increasing due to the environmental context and the need for alternatives to fossil-based products like fuels. Biodiesel, as an alternative to diesel, is a well-developed solution that is perfectly suitable in the environmental context as it is produced from renewable resources. It is also biodegradable, non-toxic, and has significantly lower greenhouse gas emissions compared to diesel. This review focuses on bioprocesses that play an important role in biodiesel production, which can achieve high yields above 90% with minimal impurities. The use of waste oils as feedstock, which accounts for 10% of biodiesel production, avoids competition with agriculture. Furthermore, the review discusses the valorization of glycerol, a by-product of biodiesel production, and techniques for its extraction and purification. Overall, this review presents the steps involved in biodiesel production using the most environmentally-friendly processes.
Article
Biochemistry & Molecular Biology
Mira Abou-Diab, Jacinthe Thibodeau, Ismail Fliss, Pascal Dhulster, Laurent Bazinet, Naima Nedjar
Summary: This study successfully produced discolored and demineralized bovine hemoglobin hydrolysates without the addition of chemical agents using an optimized multi-step process of electrodialysis with bipolar membranes (EDBM). The hydrolysates showed significant antimicrobial and antioxidant activities, making them promising natural, low-salt food preservatives.
Article
Environmental Sciences
Takwa Wakkaf, Radhouan El Zrelli, Mikael Kedzierski, Rafik Balti, Moez Shaiek, Lamjed Mansour, Sabiha Tlig-Zouari, Stephane Bruzaud, Lotfi Rabaoui
MARINE POLLUTION BULLETIN
(2020)
