Article
Food Science & Technology
Rebecca Stearns, Kristen Matak, Annette Freshour, Cangliang Shen
Summary: This study compared different methods to reduce and prevent cross-contamination of Listeria monocytogenes and L. innocua on Fuji apples. The results showed that using a dip procedure with 0.25 ml/dL H2O2-PAA was the most effective in reducing the contamination for both L. monocytogenes and L. innocua. Additionally, using a low pressure, conventional, hand-held garden sprayer with 0.25 ml/dL H2O2-PAA minimized the cross-contamination rate. The study also found that L. innocua is a suitable surrogate for L. monocytogenes on apples.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Dong-Joo Han, Su-Jin Jun, Byung-Hoo Lee, Sang-Ho Yoo
Summary: This study evaluated the cryoprotective effect of turanose on Lactobacillus paracasei subsp. paracasei, L. casei 431, and found that it significantly improved cell viability during the freeze-drying process. However, the effectiveness decreased over time and at higher temperatures.
FOOD SCIENCE AND BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Ana Beatriz Praia, Marcos Edgar Herkenhoff, Oliver Broedel, Marcus Frohme, Susana Marta Isay Saad
Summary: This study evaluated the feasibility of producing probiotic beer using F19 and US-05 in four sour beer formulations, with F19 showing better growth in formulations without juice or bagasse, while S. mombin juice or bagasse may threaten probiotic survival. Different flavors and aroma compounds were detected.
Article
Food Science & Technology
Wei Song, Panpan Hu, Shouli Guo, Jinhong Hu, Chen Song, Tianyi Wang, Zihan Gao, Tianli Yue
Summary: Colorectal cancer is the third most malignant cancer globally. This study found that M5-EPSs can induce apoptosis in HT-29 cells by activating endoplasmic reticulum stress signaling pathway, leading to growth inhibition by disrupting the redox system.
FOOD SCIENCE & NUTRITION
(2021)
Article
Chemistry, Medicinal
Yun Jeong Shin, Jung-Min Bae, Hye-Rin Cho, Patience Mahoro, Seon Hwa Kim, Min Ji Han, Min-Jung Bae
Summary: This study aimed to assess the anti-obesity properties of the probiotic strain Lactobacillus casei 431. The results showed that administration of L. casei 431 reduced body weight gain and fat accumulation, as well as decreased levels of adipose enzymes in obese rats. Furthermore, L. casei 431 promoted lipid metabolism and reduced lipid buildup.
JOURNAL OF MEDICINAL FOOD
(2023)
Article
Chemistry, Physical
Simone Savino, J. Daniel-Morah Meijer, Henriette J. Rozeboom, Hugo L. van Beek, Marco W. Fraaije
Summary: L-Amino acid oxidase (LAAO) is an enzyme that catalyzes the oxidative reaction of amino acids, with functions including amino acid detection, production of hydrogen peroxide or antimicrobial agents, and synthesis of enantiopure amino acids. This study characterized a LAAO from Pseudoalteromonas luteoviolacea, determining its substrate scope, kinetic properties, and thermostability. The crystal structure of this bacterial LAAO was also elucidated, providing insights for the development of engineered LAAO variants for biosensing and biocatalysis applications.
Article
Microbiology
Maria C. Tarifa, Cristian M. Piqueras, Diego B. Genovese, Irene A. Rubel, Maria G. Sica, Lorena Brugnoni
Summary: This study evaluated the synergistic effect of microencapsulation in pectin microgels and inulin extracted from Jerusalem artichoke on the survival of Lactobacillus paracasei subsp. tolerans F2 in Oncorhynchus mykiss. The results showed that encapsulation in pectin microgels improved the strain's survival during storage and exposure to simulated gastrointestinal conditions, especially when combined with inulin from Jerusalem artichoke. These findings suggest the potential use of these techniques for developing functional feeds with improved viability and digestibility.
REVISTA ARGENTINA DE MICROBIOLOGIA
(2022)
Article
Biochemistry & Molecular Biology
Arlett L. Ibarra-Villarreal, Maria Fernanda Villarreal-Delgado, Fannie Isela Parra-Cota, Enrico A. Yepez, Carlos Guzman, Marco Antonio Gutierrez-Coronado, Luis Carlos Valdez, Carolina Saint-Pierre, Sergio de Los Santos-Villalobos
Summary: A field experiment was conducted to investigate the effect of a native bacterial inoculant on the growth, yield, and quality of wheat. Results showed that the application of the bacterial consortium enhanced wheat yield without compromising grain quality. This study suggests that the use of this bacterial consortium has the potential to improve wheat production.
PLANT SIGNALING & BEHAVIOR
(2023)
Article
Nutrition & Dietetics
Miguel Ribeiro, Joana Costa, Isabel Mafra, Sandra Cabo, Ana Paula Silva, Berta Goncalves, Melanie Hillion, Michel Hebraud, Gilberto Igrejas
Article
Biology
Carolina Sabenca, Telma de Sousa, Soraia Oliveira, Didier Viala, Laetitia Theron, Christophe Chambon, Michel Hebraud, Racha Beyrouthy, Richard Bonnet, Manuela Canica, Patricia Poeta, Gilberto Igrejas
Article
Biochemical Research Methods
Ricardo Monteiro, Ingrid Chafsey, Valentin Ageorges, Sabine Leroy, Christophe Chambon, Michel Hebraud, Valerie Livrelli, Mariagrazia Pizza, Alfredo Pezzicoli, Mickael Desvaux
Summary: This study provides the first comprehensive view of the secretome landscape of E. coli O157:H7, highlighting the significant influence of environmental conditions on the extracytoplasmic proteome and identifying important molecular targets for preventive strategies against EHEC/STEC.
JOURNAL OF PROTEOMICS
(2021)
Review
Biochemistry & Molecular Biology
Telma de Sousa, Michel Hebraud, Maria L. N. Enes Dapkevicius, Luis Maltez, Jose Eduardo Pereira, Rosa Capita, Carlos Alonso-Calleja, Gilberto Igrejas, Patricia Poeta
Summary: In recent years, the effectiveness of antimicrobials in the treatment of Pseudomonas aeruginosa infections has decreased, with its high pathogenicity, multi-resistance, and biofilm production posing challenges for treatment. Deeper understanding of its pathogenicity factors and development of more targeted therapies are needed for effective treatment against this pathogen.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Gastroenterology & Hepatology
Clara Douadi, Emilie Vazeille, Christophe Chambon, Michel Hebraud, Margot Fargeas, Marie Dodel, Dilek Coban, Bruno Pereira, Aurelien Birer, Pierre Sauvanet, Anthony Buisson, Nicolas Barnich
Summary: Anti-TNF agents limit the replication of pathobionts, such as AIEC, within macrophages in CD patients by modulating the expression of FLOT-1 and CHI3L1.
JOURNAL OF CROHNS & COLITIS
(2022)
Article
Biochemical Research Methods
Ingrid Chafsey, Rafal Ostrowski, Morgan Guilbaud, Pilar Teixeira, Jean-Marie Herry, Nelly Caccia, Christophe Chambon, Michel Hebraud, Joana Azeredo, Marie-Noelle Bellon-Fontaine, Magdalena Popowska, Mickael Desvaux
Summary: Listeria monocytogenes exhibits a dimorphism associated with SecA2 activity, with significant implications for ecophysiology and pathophysiology, affecting cell surface properties and proteosurfaceome composition.
JOURNAL OF PROTEOMICS
(2022)
Article
Multidisciplinary Sciences
Benjamin Felix, Yann Sevellec, Federica Palma, Pierre Emmanuel Douarre, Arnaud Felten, Nicolas Radomski, Ludovic Mallet, Yannick Blanchard, Aurelie Leroux, Christophe Soumet, Arnaud Bridier, Pascal Piveteau, Eliette Ascensio, Michel Hebraud, Renata Karpiskova, Tereza Gelbicova, Marina Torresi, Francesco Pomilio, Cesare Camma, Adriano Di Pasquale, Taran Skjerdal, Ariane Pietzka, Werner Ruppitsch, Monica Ricao Canelhas, Bojan Papic, Ana Hurtado, Bart Wullings, Hana Bulawova, Hanna Castro, Miia Lindstrom, Hannu Korkeala, Zanete Steingolde, Toomas Kramarenko, Lenka Cabanova, Barbara Szymczak, Manfred Gareis, Verena Oswaldi, Elisabet Marti, Anne-Mette Seyfarth, Jean-Charles Leblanc, Laurent Guillier, Sophie Roussel
Summary: Listeria monocytogenes is a common bacterium that causes serious foodborne illness. Understanding its adaptation mechanisms in food is important for improving food safety. A dataset of genomes from Lm strains collected in 19 European countries was constructed, providing insights into Lm ecology and aiding in surveillance efforts. This dataset serves as a basis for discovering genetic traits underlying Lm adaptation to different ecological niches.
Article
Microbiology
Laurianne Paris, Marion Devers-Lamrani, Muriel Joly, Didier Viala, Marie De Antonio, Bruno Pereira, Nadine Rouard, Pascale Besse-Hoggan, Michel Hebraud, Edward Topp, Fabrice Martin-Laurent, Isabelle Batisson
Summary: The transcriptomic and proteomic responses of Microbacterium sp. C448 exposed to sulfamethazine antibiotic were evaluated in this study. It was found that the therapeutic concentration induced the highest expression and production of Sad, which is consistent with the observed SMZ degradation activity. Additionally, two novel factors involved in 4-aminophenol metabolite degradation and the export of sulfate residues formed during SMZ degradation were discovered.
FEMS MICROBIOLOGY ECOLOGY
(2023)
Article
Cell Biology
Ricardo Monteiro, Ingrid Chafsey, Nelly Caccia, Valentin Ageorges, Sabine Leroy, Didier Viala, Michel Hebraud, Valerie Livrelli, Mariagrazia Pizza, Alfredo Pezzicoli, Mickael Desvaux
Summary: Escherichia coli is a versatile species found in the animal gut that can be both a harmless commensal and a pathogenic organism causing infections. Its genetic flexibility has led to the evolution of pathogenic strains that pose a threat to global health, as well as antibiotic resistance. Despite the challenges in vaccine development due to the heterogeneity of colonization factors, targeting conserved proteins such as OmpA could be an effective approach. This study identified OmpA as a highly conserved and immunogenic antigen that plays a significant role in adhesion and aggregation of E. coli O157:H7, highlighting its potential as a potent antigen for vaccine development against intestinal pathogenic E. coli.
Article
Microbiology
Telma de Sousa, Michel Hebraud, Olimpia Alves, Eliana Costa, Luis Maltez, Jose Eduardo Pereira, Angela Martins, Gilberto Igrejas, Patricia Poeta
Summary: A study conducted at the Medical Center of Tras-os-Montes and Alto Douro (CHTMAD) found that Pseudomonas aeruginosa strains causing urinary tract infections are resistant to multiple antibiotics and have high biofilm formation and motility capabilities, posing a serious public health problem.
Article
Veterinary Sciences
Telma de Sousa, Andreia Garces, Augusto Silva, Ricardo Lopes, Nuno Alegria, Michel Hebraud, Gilberto Igrejas, Patricia Poeta
Summary: This study evaluated the antimicrobial resistance patterns and biofilm production of clinical isolates of pathogenic bacterium Pseudomonas aeruginosa in dogs. The results revealed widespread resistance to beta-lactam antimicrobials, with amikacin and tobramycin being the only effective aminoglycosides. All isolates carried the oprD gene and virulence genes. The study emphasizes the importance of monitoring antimicrobial resistance and responsible antibiotic use in veterinary medicine to prevent multi-drug resistance.
VETERINARY SCIENCES
(2023)
Article
Microbiology
Telma de Sousa, Catarina Silva, Olimpia Alves, Eliana Costa, Gilberto Igrejas, Patricia Poeta, Michel Hebraud
Summary: This study investigates the susceptibility of Pseudomonas aeruginosa isolates from septicemias to various antibiotics, specifically imipenem + cilastatin and tetracycline, as well as the impact of these treatments on biofilm formation. The results reveal that most isolates are sensitive to six antibiotics, but resistance is observed in some antibiotics. The study also explores different resistance genes and virulence genes in P. aeruginosa isolates.
Article
Biotechnology & Applied Microbiology
Sadika Dkhili, Miguel Ribeiro, Salma Ghariani, Houssem Ben Yahia, Melanie Hillion, Patricia Poeta, Karim Ben Slama, Michel Hebraud, Gilberto Igrejas
Summary: This study analyzed the structure of three lytic bacteriophages infecting Escherichia coli and the cellular response of this bacterium to phage infection at the proteome level using gel-based proteomics approach. The results indicated that viral infection caused bacterial oxidative stress and bacterial cells response to stress was orchestrated by antioxidant defense mechanisms. Further research on bacteriophages as potential antimicrobial agents is called for in the context of planetary health and integrative biology amidst converging ecological threats in the 21st century.
OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY
(2021)
Meeting Abstract
Gastroenterology & Hepatology
Clara Douadi, Emilie Vazeille, Christophe Chambon, Michel Hebraud, Marie Dodel, Dilek Coban, Bruno Pereira, Aurelien Birer, Pierre Sauvanet, Anthony Buisson, Nicolas Bamich
Article
Biology
Telma de Sousa, Didier Viala, Laetitia Theron, Christophe Chambon, Michel Hebraud, Patricia Poeta, Gilberto Igrejas