Article
Biophysics
Alina Vasilescu, Borys Hrinczenko, Greg M. Swain, Serban F. Peteu
Summary: This mini-review introduces the reader to research on exhaled breath analysis for detecting abnormal chemical markers reflective of respiratory diseases. It discusses two main groups of sensing methods: mass spectrometry and (bio)sensors, with a focus on biosensor applications for EB and EBC analyses. The review concludes with recommendations for future development of EBC biomarker sensing in the near and long term.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Health Care Sciences & Services
Carlos Perez-Sanchez, Nuria Barbarroja, Lucas C. Pantaleao, Laura M. Lopez-Sanchez, Susan E. Ozanne, Bernabe Jurado-Gamez, Enrique Aranda, Chary Lopez-Pedrera, Antonio Rodriguez-Ariza
Summary: This study provides evidence for the clinical utility of miRNAs from exhaled breath condensate as biomarkers of lung cancer. Genome-wide miRNA profiling and machine learning analysis revealed altered miRNA levels in lung cancer patients, with specific signatures accurately distinguishing patient types and predicting clinical outcomes. Dysregulated EBC miRNAs also showed associations with clinical features and potential target genes related to lung cancer pathogenesis.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Review
Chemistry, Analytical
Qiwen Yu, Jing Chen, Wei Fu, Kanhar Ghulam Muhammad, Yi Li, Wenxin Liu, Linxin Xu, Hao Dong, Di Wang, Jun Liu, Yanli Lu, Xing Chen
Summary: Lung cancer, with its high morbidity and mortality, has been studied for decades. Traditional methods for diagnosis are invasive and expensive, hindering early screening and diagnosis. Smartphones integrated with powerful applications show great potential for improving the detection and monitoring of lung cancer markers in various medical settings.
Article
Medicine, General & Internal
Inger Lise Gade, Jacob Gammelgaard Schultz, Rasmus Froberg Brondum, Benedict Kjaergaard, Jens Erik Nielsen-Kudsk, Asger Andersen, Soren Risom Kristensen, Bent Honore
Summary: This study investigated the proteome of exhaled breath condensate in a porcine model of acute PE to identify potential diagnostic markers. Proteins in the EBC of pigs with PE showed significant alterations, with albumin and neutrophil gelatinase-associated lipocalin being potential diagnostic markers for PE. A prediction model based on penalized regression accurately discriminated between baseline and PE samples, suggesting a new approach for early PE diagnosis.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Cell Biology
Mauro Maniscalco, Debora Paris, Paola Cuomo, Salvatore Fuschillo, Pasquale Ambrosino, Annabella Tramice, Letizia Palomba, Andrea Motta
Summary: Chronic obstructive pulmonary disease (COPD) is a heterogeneous disease, and a single strategy of pulmonary rehabilitation (PR) may not achieve the desired outcomes for all patients. This study used nuclear magnetic resonance (NMR) profiling of exhaled breath condensate (EBC) and multivariate statistical analysis to investigate the relationship between biomarkers and clinical parameters in COPD patients undergoing PR. The results showed that the metabolic COPD phenotype evolves during PR, and methanol metabolite could serve as a useful biomarker for monitoring personalized outcomes.
Article
Oncology
Jiatao Liao, Yuan Li, Chang Liu, Qianqian Long, Jialei Wang
Summary: This case report highlights a rare histological transformation from EGFR-mutated lung ADC to SCC after TKI treatment, suggesting a possible mechanism of EGFR inhibition resistance with SCC transition and EGFR amplification loss. The optimal post-progression therapy for ADC-SCC patients remains challenging and requires further studies.
FRONTIERS IN ONCOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Federica Ghelli, Marco Panizzolo, Giacomo Garzaro, Giulia Squillacioti, Valeria Bellisario, Nicoletta Colombi, Enrico Bergamaschi, Irina Guseva Canu, Roberto Bono
Summary: This systematic review compares baseline values of pro/anti-inflammatory biomarkers in Exhaled Breath Condensate (EBC) in healthy, non-smoking adults, aiming to summarize the concentrations reported in the literature. The adherence to guidelines is crucial due to differences in methodologies employed in the included articles concerning EBC sampling, storage, and analyses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Shuo Wei, Yi-sheng Chen, Yi Shi
Summary: This study identified potential biomarkers for the diagnosis of pulmonary aspergillosis (PA) by analyzing the metabolic profile of exhaled breath condensate (EBC) samples. The identified compounds showed good classification between PA patients and controls, and can serve as noninvasive markers for the diagnosis of PA.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Sabine Szunerits, Hannes Doerfler, Quentin Pagneux, John Daniel, Shekhar Wadekar, Eloise Woitrain, Dennis Ladage, David Montaigne, Rabah Boukherroub
Summary: Since the outbreak of the SARS-CoV-2 pandemic, the analysis of exhaled breath has gained attention as a non-invasive diagnostic approach. Mass spectrometry has been recognized as a powerful tool for clinical diagnostics, but its use is limited in the clinical setting. Recent developments focus on online sampling using portable and wearable devices, such as face masks, which have the potential to enable fast and non-invasive diagnosis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Oncology
Daniel J. Ryan, Sinead Toomey, Robert Smyth, Stephen F. Madden, Julie Workman, Robert Cummins, Katherine Sheehan, Joanna Fay, Jarushka Naidoo, Oscar S. Breathnach, Patrick G. Morris, Liam Grogan, Michael E. O'Brien, Imran Sulaiman, Bryan T. Hennessy, Ross K. Morgan
Summary: Small diagnostic tissue samples may not be sufficient to detect oncogenic driver mutations in lung cancer, while liquid biopsies may provide better reflection of tumor heterogeneity. This study found that compared to tissue NGS, both plasma and exhaled breath condensate (EBC) identified a higher number of mutations, and there were differences in mutation profiles between plasma and EBC.
Review
Medicine, General & Internal
Joanna Polomska, Kamil Bar, Barbara Sozanska
Summary: This paper provides an overview of the pathophysiology of asthma and the potential of exhaled breath condensate (EBC) as a source of biomarkers for lung disorders. It discusses the clinical implications and limitations of using EBC in asthma research.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Toxicology
Jean-Jacques Sauvain, Maud Hemmendinger, Guillaume Suarez, Camille Creze, Nancy B. Hopf, Valerie Jouannique, Amelie Debatisse, Jacques A. Pralong, Pascal Wild, Irina Guseva Canu
Summary: Exposure to subway particulate matter may alter concentrations of nitrogen oxides, acetate, and lactate in the exhaled breath condensate of subway workers. The effect is influenced by particle size and could reflect the body's response to oxidative stress in order to maintain redox and/or metabolic homeostasis.
PARTICLE AND FIBRE TOXICOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Maud Hemmendinger, Jean-Jacques Sauvain, Nancy B. Hopf, Pascal Wild, Guillaume Suarez, Irina Guseva Canu
Summary: The study optimized a method based on 2,4-dinitrophenylhydrazine for quantifying MDA in EBC, with analytical uncertainty at the LOQ level reaching 23%, yet still lower than the observed inter-individual variability.
Article
Oncology
Naseer Ahmed, Biniam Kidane, Le Wang, Zoann Nugent, Nataliya Moldovan, April McElrea, Shiva Shariati-Ievari, Gefei Qing, Lawrence Tan, Gordon Buduhan, Sadeesh K. Srinathan, Renelle Meyers, Michel Aliani
Summary: In this pilot study, metabolic alterations in sputum and breath condensate of early-stage lung cancer patients before and after surgery were analyzed. The results suggest that these metabolic changes could serve as noninvasive diagnostic tools for early detection of lung cancer and potential targets for treatment response and drug discovery.
FRONTIERS IN ONCOLOGY
(2022)
Review
Medicine, General & Internal
Janette Greenhalgh, Angela Boland, Victoria Bates, Fabio Vecchio, Yenal Dundar, Marty Chaplin, John A. Green
Summary: EGFR mutation positive non-small cell lung cancer is more common in women and occurs at a younger age. Treatment with single-agent or combination EGFR therapies shows advantages in improving tumor response rate and prolonging progression-free survival compared to other cytotoxic chemotherapy agents.
COCHRANE DATABASE OF SYSTEMATIC REVIEWS
(2021)
Review
Allergy
Silvana Fiorito, Marzia Soligo, Yadong Gao, Ismail Ogulur, Cezmi A. Akdis, Sergio Bonini
Summary: Research suggests that the high incidence and increased mortality of COVID-19 in Northern Italy may be attributed to severe air pollution and disrupted epithelial barriers. The region's high levels of fine particulate matter contribute to increased susceptibility to the virus, while barrier dysfunction caused by air pollution can lead to chronic inflammation and impaired immune response. These factors also contribute to the higher prevalence of comorbidities and therefore increased mortality from COVID-19.
Article
Allergy
Marek Jutel, Maria J. Torres, Oscar Palomares, Cezmi A. Akdis, Thomas Eiwegger, Eva Untersmayr, Domingo Barber, Magdalena Zemelka-Wiacek, Anna Kosowska, Elizabeth Palmer, Stefan Vieths, Vera Mahler, Walter G. Canonica, Kari Nadeau, Mohamed H. Shamji, Ioana Agache
Summary: Immune modulation is a crucial treatment approach for allergic diseases, asthma, and autoimmunity. The COVID-19 vaccine has the potential to combat the global pandemic, but its administration needs to be considered in conjunction with allergen immunotherapy and biologicals.
Article
Chemistry, Multidisciplinary
Sen Wang, Li Zhang, Pengfei Wang, Xingchen Liu, Yanyan Chen, Zhangfeng Qin, Mei Dong, Jianguo Wang, Lin He, Unni Olsbye, Weibin Fan
Summary: Converting CO2 into light olefins is a promising approach, but achieving high selectivity for specific olefins remains a challenge. Researchers have developed a new catalyst that exhibits a C-2(=)-C-4(=) selectivity of 95.7% at high temperature and pressure, with an ethene/propene ratio three times that of previous results. The catalyst shows excellent stability and the reaction mechanism has been elucidated through experiments and calculations.
Article
Crystallography
Shuang Wang, Minghui Yu, Hao Yu, Yuye Cheng, Minghao Dou, Xiaoyu Gong, Zhiqiang Li, Hongyu Shao, Guangyu Chen, Shenjie Li, Yanyan Chen
Summary: In this study, CdS/Ag2S heterojunction composite photocatalyst was fabricated through a simple hydrothermal method. The photoreactivity and stability of the composite structure were evaluated, and it was found that the CdS-Ag2S=1:1 composition showed higher photocatalytic activity and stability.
CRYSTAL RESEARCH AND TECHNOLOGY
(2022)
Article
Oncology
Guanghao Wu, Biying Chen, Junjie Jiang, Yiran Chen, Yanyan Chen, Haiyong Wang
Summary: This study reveals the predictive capability of pyroptosis in immunotherapy for melanoma. By developing a pyroptosis-based model, it is reliable to predict the clinical benefits and survival outcomes of melanoma patients receiving immunotherapy.
Article
Oncology
Yuchu Zhao, Shiting Yu, Yue Wang, Yanyan Chen, Jingjing Chen, Jiawen Wang, Meichen Liu, Siming Wang
Summary: The study found that the water-soluble total protein extract from Pueraria Lobata Radix (PLP) had a strong inhibitory effect on tyrosinase activity and melanin synthesis in B16 melanoma cells. Mechanistically, PLP downregulated the expression of MITF-related melanogenic enzymes and induced apoptosis through mitochondria-related pathways. Additionally, PLP regulated antioxidant enzymes to reduce reactive oxygen species levels and inhibit melanogenesis.
MOLECULAR MEDICINE REPORTS
(2023)
Article
Nanoscience & Nanotechnology
Hao Yu, Minghao Dou, Shuang Wang, Yuye Cheng, Zhiqiang Li, Hongyu Shao, Mengjie Yao, Xinrui Wang, Yanyan Chen, Shenjie Li
Summary: A convenient and safe coordination-assisted self-assembly method was used to encapsulate quantum dots into metal-organic frameworks and further formed AgInS2@ZIF-8/ZnS nanostructures. This improved their photocatalytic hydrogen production performance and cycling stability.
ACS APPLIED NANO MATERIALS
(2023)
Article
Engineering, Environmental
Hongwei Li, Yuanming Lai, Huyuan Zhang, Yanyan Chen, Wangtao Jiang, Yan Tian, Jiachuan Ran
Summary: This paper presents a refined closed-form solution for evaluating the mechanical behavior of the composite lining-ground interaction in cold-region tunnels. The solutions for moment, thrust, radial displacement, and contact stress have been derived based on the unified strength theory. The usefulness of this method in tackling ground-structure interaction problems in cold regions is demonstrated through studying the equations for the lining-ground interaction behavior.
COLD REGIONS SCIENCE AND TECHNOLOGY
(2023)
Article
Energy & Fuels
Tianqing Zhou, Shaoze Wang, Chaojian Zhang, Yue Yao, Yanyan Chen, Shuxiang Lu, Xiaoyuan Liao
Summary: Improving catalysts' desulfurization capacity is crucial for environmental protection. NH2-MIL-125 material, a MOF material with good adsorption properties, was prepared using a hydrothermal method. The dried materials at different temperatures were analyzed using XRD, FT-IR, SEM, and physical adsorption. Experiment results showed that NH2-MIL-125@150 degrees C had the highest desulfurization capacity, followed by NH2-MIL-125@200 degrees C and NH2-MIL-125@250 degrees C, while NH2-MIL-125@110 degrees C had the lowest capacity.
Article
Oncology
Yanyan Chen, Guoxin Cai, Junjie Jiang, Chao He, Yiran Chen, Yongfeng Ding, Jun Lu, Wenyi Zhao, Yan Yang, Yiqin Zhang, Guanghao Wu, Haiyong Wang, Zhan Zhou, Lisong Teng
Summary: This study examined the heterogeneity of primary and peritoneal metastasis (PM) samples in gastric cancer (GC) patients using quantitative proteomics and whole-exome sequencing. Proteomic features enriched in RNA binding and extracellular exosomes were observed in PM samples. A ten-protein signature was derived by machine learning, which was significantly associated with patient prognosis. This signature could guide risk stratification and individualized treatment.
Article
Engineering, Geological
Hongwei Li, Yuanming Lai, Huyuan Zhang, Yanyan Chen, Wangtao Jiang, Jiachuan Ran
Summary: A refined closed-form analytical solution is developed to evaluate the mechanical behavior of composite lining-ground interaction under the coupling effects of anisotropic frost heave and dynamic loads in cold regions. The composite lining is considered homogenized, consisting of an inner thin-walled shell and an outer thick-walled cylinder embedded in an isotropic medium. The proposed solutions, based on elasticity theory, are more suitable for predicting the mechanical behavior of composite lining-ground interaction under anisotropic frost action. The analytical solutions can address soil-structure interaction problems in cold regions.
INTERNATIONAL JOURNAL OF ROCK MECHANICS AND MINING SCIENCES
(2023)
Article
Nanoscience & Nanotechnology
Yanyan Chen, Shenjie Li, Zhiqiang Li, Wanqing Li, Hongyu Shao, Minghao Dou, Yuye Cheng, Xiangling Wan, Xiuxian Jiang, Zhengguang Zhang
Summary: Water-soluble nonrare metal Ag-Sn-S nanocrystals (ATS NCs) were synthesized at room temperature using the interfacial nucleation mechanism. The synthesized ATS NCs exhibited a band gap of 2.32 to 2.59 eV, giving them a high redox ability and very high photocatalytic degradation rate. The interfacial regulation of acid protonation on the surface of ATS NCs resulted in more defect states, which improved the photocatalytic oxidation degradation efficiency. The ATS NCs showed potential for practical treatment of large-scale industrial wastewater.
ACS APPLIED NANO MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Fung-Kit Tang, Yanyan Chen, Daniel Nnaemaka Tritton, Zongwei Cai, Ken Cham-Fai Leung
Summary: A class of rhodamine-based fluorescent sensors that can selectively and sensitively detect Pd2+ metal ions in aqueous media have been developed. These sensors, PMS and PRS, exhibit colorimetric and fluorescent changes when exposed to Pd2+, due to the opening and restoring of their spirolactam rings. PRS has high selectivity for Pd2+ over 22 other metal ions, with a 0.6-fold ratiometric difference at I-600nm/I-515nm. The closed form of the lactam ring in PRS-Pd can be switched back in the presence of various thiols, providing a red-green traffic light detection mechanism. PRS also shows excellent cell viability and can be used to image Pd2+ and detect biothiols in cancer cells.
CHEMISTRY-AN ASIAN JOURNAL
(2023)
Article
Chemistry, Physical
Yinghao Zhao, Hualan Zhou, Mengzhen Song, ZeXin Xu, Zhiwei Sun, Qianhui Xu, Yanyan Chen, Xiaoyuan Liao
Summary: Adsorption technology is a promising method for removing pollutants in fluids, and Ti-MOFs are widely used as efficient adsorbents. However, the use of Ti-MOFs with different morphologies for dye removal has not been reported. In this study, three kinds of Ti-MOFs with different morphologies were prepared and tested for adsorbing different dyes. It was found that the truncated octahedron Ti-MOFs showed the best removal efficiency for the cationic dye methylene blue. The adsorption process was exothermic and followed a pseudo-second-order/Freundlich isotherm model. The results also showed that the adsorption performance was dependent on the facet orientation, with truncated octahedron Ti-MOFs exhibiting the highest adsorption capacity among the three morphologies. This research provides a highly efficient adsorbent for industrial dye wastewater remediation.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Article
Chemistry, Multidisciplinary
Yingjie Li, Penghui Li, Chaojian Zhang, Kai He, Yanyan Chen, Xiaoyuan Liao
Summary: The dual Zn source (DZS) strategy was used to prepare high-quality and high-output ZIF-8, eliminating waste acid and improving the yield. The resulting ZIF-8 had a large surface area (1571 m(2) g(-1)) and a unique diamond dodecahedron morphology, showing excellent adsorption desulfurization ability (19 mg g(-1)). The introduction of Zn(OH)(2) or ZnO facilitated the diffusion of the LUMO orbital, enhancing the electron accepting ability and accelerating the formation of ZIF-8 crystals. Most importantly, the DZS method allowed for the direct reuse of the resultant mother liquor, achieving zero-discharge.
