期刊
LUMINESCENCE
卷 26, 期 2, 页码 86-92出版社
WILEY
DOI: 10.1002/bio.1188
关键词
CdTe-CdS QDs; immuno-labeling; cell imaging; L-cysteine; HeLa cell
资金
- National Science Foundation of China [20875011, 20675012, 20635010]
Functionalized CdTe-CdS core-shell quantum dots (QDs) were synthesized in aqueous solution via water-bathing combined hydrothermal method using L-cysteine (L-Cys) as a stabilizer. This method possesses both the advantages of water-bathing and hydrothermal methods for preparing high-quality QDs with markedly reduced synthesis time, and better stability than a lone hydrothermal method. The QDs were characterized by transmission electronic microscopy and powder X-ray diffraction and X-ray photoelectron spectroscopy. The CdTe-CdS QDs with core-shell structure showed both enhanced fluorescence and better photo stability than nude CdTe QDs. After conjugating with antibody rabbit anti-CEACAM8 (CD67), the as-prepared L-Cys capped CdTe-CdS QDs were successfully used as fluorescent probes for the direct immuno-labeling and imaging of HeLa cells. It was indicated that this kind of QD would have application potential in bio-labeling and cell imaging. Copyright (c) 2009 John Wiley & Sons, Ltd.
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