Article
Biochemistry & Molecular Biology
Lily Southcott, Luke Wharton, Julie Rousseau, Helen Merkens, Hua Yang, Francois Benard, Chris Orvig
Summary: A new bifunctional chelating ligand was synthesized and conjugated to Trastuzumab for immuno-PET imaging. The ligand showed improved stability and lower demetallation compared to a previously synthesized ligand. The conjugate was assessed using various radiolabeling and imaging techniques.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Naomi S. Sta Maria, Leslie A. Khawli, Vyshnavi Pachipulusu, Sharon W. Lin, Long Zheng, Daniel Cohrs, Xiaodan Liu, Peisheng Hu, Alan L. Epstein, Russell E. Jacobs
Summary: This study demonstrates the use of Zr-89-oxine PET imaging to monitor the biodistribution of CAR T cells in vivo, revealing dose-dependent distribution patterns and a surprising lung-liver-spleen migration pathway for the cells post-injection. These findings underscore the importance of real-time monitoring for interpreting treatment efficacy and guiding patient care.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Lisa Russelli, Francesco De Rose, Loredana Leone, Sybille Reder, Markus Schwaiger, Calogero D'Alessandria, Lorenzo Tei
Summary: In this study, a new chelator AAZTHAG was designed, developed, and utilized for Zr-89 labeling and PET imaging. The high stability and good biodistribution of AAZTHAG in vivo were confirmed through in vitro and in vivo tests. Additionally, the bifunctionality of AAZTHAG was demonstrated by conjugating it with the human anti-HER2 monoclonal antibody Trastuzumab.
Article
Medicine, Research & Experimental
Ge Yan, Xinyu Wang, Yeli Fan, Jianhan Lin, Junjie Yan, Lizhen Wang, Donghui Pan, Yuping Xu, Min Yang
Summary: This study used immuno-positron emission tomography (PET) imaging to label TNF-alpha and discovered an increased level of TNF-alpha in the inflammatory colon of mice. The results showed a significantly higher uptake rate in the inflammatory colon compared to the control group, suggesting the potential for this method in diagnosing and predicting efficacy in IBD and other autoimmune diseases.
MOLECULAR PHARMACEUTICS
(2022)
Article
Pharmacology & Pharmacy
Andras Polyak, Jens P. Bankstahl, Karen F. W. Besecke, Constantin Hozsa, Wiebke Triebert, Rajeswara Rao Pannem, Felix Manstein, Thomas Borcholte, Marcus Furch, Robert Zweigerdt, Robert K. Gieseler, Frank M. Bengel, Tobias L. Ross
Summary: This study optimized a method for the preparation of the highly lipophilic labeling synthon [Zr-89]Zr(oxinate)(4) for radiolabeling of liposomes and human induced pluripotent stem cells. The developed labeling protocol demonstrated high radiochemical yields and efficient cell internalization rates. Prolonged PET imaging confirmed the stability of labeled nanocarriers in vivo, emphasizing the potential of the method for drug delivery and cell therapy applications.
Article
Oncology
Arjan Kol, Xiaoyu Fan, Marta. A. A. Wazynska, Sander M. J. van Duijnhoven, Danique Giesen, Annechien Plat, Hans Van Eenennaam, Philip. H. H. Elsinga, Hans. W. W. Nijman, Marco de Bruyn
Summary: This study focuses on generating and radiolabeling monoclonal antibodies targeting human CD103 for non-invasive immune PET imaging, demonstrating their potential as biomarkers for effective anticancer immune responses. The results show high specificity and sensitivity of the CD103 immuno-PET tracers in visualizing T cell infiltration, indicating their suitability for future applications in cancer immunotherapy assessment.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Biochemistry & Molecular Biology
Jacinta Jacob, Alessia Volpe, Qi Peng, Robert I. Lechler, Lesley A. Smyth, Giovanna Lombardi, Gilbert O. Fruhwirth
Summary: Regulatory T cells (Tregs) are a potential cell therapy for autoimmune diseases and transplant rejection. However, the fate and function of Tregs in vivo remain unclear. In this study, we attempted to track Tregs using radiolabeling and PET imaging but found that the labeled Tregs failed to expand and survive. Therefore, indirect labeling methods may be more effective for tracking Tregs in this scenario.
Article
Oncology
David Bauer, Hannah Visca, Anuradha Weerakkody, Lukas M. Carter, Zachary Samuels, Spencer Kaminsky, Oleg A. Andreev, Yana K. Reshetnyak, Jason S. Lewis
Summary: This study identified a promising acidity-imaging PET probe for clinical translation, which can quantitatively visualize the acidic tumor microenvironment via positron emission tomography without relying on tumor-specific molecular markers.
FRONTIERS IN ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Logan D. Stone, Adriana V. F. Massicano, Todd M. Stevens, Jason M. Warram, Anthony B. Morlandt, Suzanne E. Lapi, Hope M. Amm
Summary: Accurate assessment of tumor margins using non-invasive imaging is crucial for preserving healthy tissue and improving long-term tumor control. This study investigates the use of epidermal growth factor receptor (EGFR) as an imaging biomarker for ameloblastomas. The results show that radiolabeled anti-EGFR exhibits specificity for ameloblastoma PDX tumor xenografts, making it a promising target for pre-surgical imaging. This technology could enhance the accuracy of tumor margin assessment for the surgical removal of ameloblastomas.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Jin Hee Lee, Kyung-Ho Jung, Mina Kim, Kyung-Han Lee
Summary: This study successfully labeled Zr-89-anti-CD25 IgG to sulfhydryl moieties and verified its specific binding capacity in human T-cell lymphoma. Pharmacokinetic and biodistribution studies confirmed the specific uptake of Zr-89-CD25 IgG in lymphoma tumors. Furthermore, the uptake in non-tumor tissues was minimal and not influenced by circulating soluble CD25 protein.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Helen Damerow, Xia Cheng, Valeska von Kiedrowski, Ralf Schirrmacher, Bjoern Waengler, Gert Fricker, Carmen Waengler
Summary: Zr-89 is a highly favorable positron emitter for immuno-PET imaging. Complexation of the Zr-89(4+) ion with desferrioxamine B is commonly used to introduce the ion into antibodies, but the complexes have limited kinetic inertness. Therefore, new chelators, such as DFO* and 3,4,3-(LI-1,2-HOPO), have been developed. Comparisons between these chelators have shown that DFO* is the most suitable candidate for stable Zr-89 labeling of antibodies for clinical translation.
Article
Biochemical Research Methods
Azalea A. Khan, Francis Man, Farid N. Faruqu, Jana Kim, Fahad Al-Salemee, Amaia Carrascal-Minino, Alessia Volpe, Revadee Liam-Or, Paul Simpson, Gilbert O. Fruhwirth, Khuloud T. Al-Jamal, Rafael T. M. de Rosales
Summary: This study explored the use of [Zr-89]Zr(oxinate)(4) for labeling sEVs and demonstrated the possibility of tracking labeled sEVs in vivo using PET imaging. Differential biodistribution of intentionally heat-damaged sEVs compared to intact sEVs was also observed. Therefore, this method can be reliably used to explore the potential of sEVs as drug delivery systems.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Felix Dietlein, Carsten Kobe, Sergio Munoz Vazquez, Thomas Fischer, Heike Endepols, Melanie Hohberg, Manuel Reifegerst, Bernd Neumaier, Klaus Schomaecker, Alexander E. Drzezga, Markus Dietlein
Summary: The study found that Zr-89-PSMA-DFO can detect low-expressed PSMA prostate cancer lesions more sensitively and reliably than existing tracers in PET/CT scans. This research is of great significance in helping patients with undetectable lesions.
JOURNAL OF NUCLEAR MEDICINE
(2022)
Article
Multidisciplinary Sciences
Danique Giesen, Marjolijn N. Lub-de Hooge, Marcel Nijland, Helen Heyerdahl, Jostein Dahle, Elisabeth G. E. de Vries, Martin Pool
Summary: In this study, [Lu-177]Lu-DOTA-NNV003 was developed as a novel radioimmunotherapy (RIT) for B cell non-Hodgkin's lymphoma (NHL) patients. The use of [Zr-89]Zr-N-sucDf-NNV003 PET imaging accurately predicted the whole-body distribution and tumor uptake of [Lu-177]Lu-DOTA-NNV003. The results showed that [Zr-89]Zr-N-sucDf-NNV003 PET imaging could serve as a useful tool to predict the CD37-targeting effect of [Lu-177]Lu-DOTA-NNV003.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Pierre Cheung, Olof Eriksson
Summary: Diabetes is a chronic metabolic disease that affects over 400 million people globally, with traditional diagnostic methods having limitations in accurately reflecting disease progression. Studying beta-cell mass is crucial for understanding disease processes, but invasive procedures are required. Nuclear medical imaging techniques offer a non-invasive approach for studying beta-cell mass, potentially leading to breakthroughs in diabetes research and therapies.
Article
Oncology
Kingsley O. Asiedu, Sho Koyasu, Lawrence P. Szajek, Peter L. Choyke, Noriko Sato
CLINICAL CANCER RESEARCH
(2017)
Article
Cell Biology
Kazuhide Sato, Noriko Sato, Biying Xu, Yuko Nakamura, Tadanobu Nagaya, Peter L. Choyke, Yoshinori Hasegawa, Hisataka Kobayashi
SCIENCE TRANSLATIONAL MEDICINE
(2016)
Article
Multidisciplinary Sciences
Meili Zhang, Bernard Wen, Olga M. Anton, Zhengsheng Yao, Sigrid Dubois, Wei Ju, Noriko Sato, David J. DiLillo, Richard N. Bamford, Jeffrey Ravetch, Thomas A. Waldmann
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Radiology, Nuclear Medicine & Medical Imaging
Kingsley O. Asiedu, Munira Ferdousi, Phuongnga T. Ton, Stephen S. Adler, Peter L. Choyke, Noriko Sato
Article
Oncology
Noriko Sato, Kate Stringaris, Jan K. Davidson-Moncada, Robert Reger, Stephen S. Adler, Cynthia Dunbar, Peter L. Choyke, Richard W. Childs
CLINICAL CANCER RESEARCH
(2020)
Article
Cell & Tissue Engineering
John W. Ostrominski, Ravi Chandra Yada, Noriko Sato, Michael Klein, Ksenia Blinova, Dakshesh Patel, Racquel Valadez, Maryknoll Palisoc, Stefania Pittaluga, Kah-Whye Peng, Hong San, Yongshun Lin, Falguni Basuli, Xiang Zhang, Rolf E. Swenson, Mark Haigney, Peter L. Choyke, Jizhong Zou, Manfred Boehm, So Gun Hong, Cynthia E. Dunbar
STEM CELLS TRANSLATIONAL MEDICINE
(2020)
Article
Hematology
So Gun Hong, Noriko Sato, Fanny Legrand, Manasi Gadkari, Michelle Makiya, Kindra Stokes, Katherine N. Howe, Shiqin Judy Yu, Nathaniel Seth Linde, Randall R. Clevenger, Timothy Hunt, Zonghui Hu, Peter L. Choyke, Cynthia E. Dunbar, Amy D. Klion, Luis M. Franco
Article
Hematology
Kate Stringaris, Robert F. Hoyt, Jan K. Davidson-Moncada, Jeremy M. Pantin, John F. Tisdale, Naoya Uchida, Lydia N. Raines, Robert Reger, Noriko Sato, Cynthia E. Dunbar, Timothy J. Hunt, Randall R. Clevenger, Allen Krouse, Mark E. Metzger, Aylin C. Bonifacino, William Telford, Peter L. Choyke, Theresa Engels, Robert E. Donahue, Richard W. Childs
Review
Radiology, Nuclear Medicine & Medical Imaging
Noriko Sato, Peter L. Choyke
Summary: Immunotherapies against cancers have shown impressive progress in the past decades, with cell-based immunotherapies and immunomodulatory molecular therapies making significant contributions. Genetic engineering techniques play a crucial role in enhancing cancer targeting properties, while research on non-invasive in vivo cell monitoring technologies is gaining increasing interest.
MOLECULAR IMAGING AND BIOLOGY
(2022)
Correction
Radiology, Nuclear Medicine & Medical Imaging
Noriko Sato, Peter L. Choyke
MOLECULAR IMAGING AND BIOLOGY
(2022)
Article
Cell Biology
Joo-Young Park, Hee Yeun Won, Devon T. DiPalma, Hye Kyung Kim, Tae-Hyoun Kim, Can Li, Noriko Sato, Changwan Hong, Ninan Abraham, Ronald E. Gress, Jung-Hyun Park
Summary: This study investigates the cytokine requirement for in vivo iNKT cells and finds that IL-7, not IL-15, is the homeostatic cytokine for iNKT cells. The abundance of IL-7 limits the size of the peripheral iNKT cell pool and there is competition for IL-7 between iNKT and conventional ab T cells.
Article
Multidisciplinary Sciences
Stephen S. Adler, Emmanuel C. Nyong, Raisa A. Glabman, Peter L. Choyke, Noriko Sato
Summary: This study assessed the use of acoustophoresis cell washing to replace manual centrifugal cell washing in radiolabeling immune cells. Results showed that acoustophoresis cell washing can produce radiolabeled cells with similar properties to the centrifugal method.
SCIENTIFIC REPORTS
(2022)
Review
Oncology
Raisa A. Glabman, Peter L. Choyke, Noriko Sato
Summary: This review summarizes the current research on cancer-associated fibroblasts (CAFs), highlights major challenges, and discusses future opportunities for improving CAF biology and targeting.
Article
Multidisciplinary Sciences
Noriko Sato, Richard N. Bamford, Bonita R. Bryant, Yutaka Tagaya, Thomas A. Waldmann
Summary: When purified, naive T cells and regulatory T cells could not proliferate to the γC-cytokines IL-2, IL-7, or IL-15, despite expressing the cognate cytokine receptors. However, dendritic cells enabled their proliferation through cell-to-cell contact, independent of T cell receptor stimulation. This preconditioning effect activated specific cellular pathways and facilitated cytokine-mediated proliferation of T cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Adam T. Waickman, Davinna L. Ligons, SuJin Hwang, Joo-Young Park, Vanja Lazarevic, Noriko Sato, Changwan Hong, Jung-Hyun Park