Article
Food Science & Technology
Wenqi Shi, Jie Li, Yanfang Chen, Yefu Chen, Xuewu Guo, Dongguang Xiao
Summary: Metabolic engineering strategies were utilized to enhance the production of C6-C10 fatty acid ethyl esters in Saccharomyces cerevisiae, including improving precursor accumulation, increasing alcohol acyltransferase activity, and facilitating ester synthesis.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Fisheries
Ka-Kei Sam, P. Merosha, M. Janaranjani, Izyan Athirah, Alexander Chong Shu-Chien
Summary: The Malaysian mahseer has the complete machinery for the biosynthesis of LC-PUFA from C18 PUFA, allowing it to utilize dietary vegetable oils for growth and deposition of n-3 LC-PUFA.
Article
Chemistry, Multidisciplinary
Weibo Wang, Qingpeng Wei, Jiayuan Zhang, Meiqi Zhang, Chuchen Wang, Renyu Qu, Yuan Wang, Guangfu Yang, Jing Wang
Summary: This study developed a genetically encoded fluorescent sensor for ratiometric quantification of LCACoAs in living cells, demonstrating conformational changes of LCACoA molecules and real-time monitoring. The research also revealed the impact of disruptions in ACSL enzymes and ACBP genes on the levels and distribution of LCACoAs in cytosol and mitochondria.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Oncology
Zhihao Jia, Xiyue Chen, Jingjuan Chen, Lijia Zhang, Stephanie N. Oprescu, Nanjian Luo, Yan Xiong, Feng Yue, Shihuan Kuang
Summary: This study identifies ACSS3 as the key enzyme for propionate metabolism and demonstrates that accumulation of propionate promotes obesity and Type 2 diabetes through triggering adipocyte autophagy.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Biotechnology & Applied Microbiology
Shiyun Li, Wenxuan Fu, Ruifang Su, Yunying Zhao, Yu Deng
Summary: In this study, Saccharomyces cerevisiae was engineered to produce malonate, a valuable chemical compound, through genetic mutations and optimization of the production process. The engineered strain showed significantly increased malonate production.
METABOLIC ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Yongjie Ma, Junyi Zha, XiangKun Yang, Qianjin Li, Qingfu Zhang, Amelia Yin, Zanna Beharry, Hanwen Huang, Jiaoti Huang, Michael Bartlett, Kaixiong Ye, Hang Yin, Houjian Cai
Summary: The study revealed that ACSL1 is highly expressed in prostate tumors, promoting the generation of fatty acyl-CoAs and facilitating cancer cell growth. ACSL1 modulates mitochondrial respiration, beta-oxidation, and ATP production, impacting fatty acid metabolism and prostate cancer progression.
Article
Oncology
Daniel L. Pouliquen, Giacomo Ortone, Letizia Rumiano, Alice Boissard, Cecile Henry, Stephanie Blandin, Catherine Guette, Chiara Riganti, Joanna Kopecka
Summary: Cross-species investigations of cancer invasiveness have identified new biomarkers for improving tumor diagnosis and prognosis. This study analyzed rat tumors and human cell lines to identify common features associated with mitochondrial proteome rewiring. The differential expression of mitochondrial proteins, particularly ACADL, was found to be associated with invasiveness in mesothelioma.
Article
Plant Sciences
Juyoung Kim, Ryeo Jin Kim, Saet Buyl Lee, Mi Chung Suh
Summary: Interactions between KCS9 and other proteins in the fatty acid elongase complex are essential for the synthesis of very-long-chain fatty acids (VLCFAs). This study provides insights into the quaternary structure and protein-protein interactions of the fatty acid elongase complex, highlighting their importance for efficient VLCFA synthesis.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Review
Biotechnology & Applied Microbiology
Shiyun Li, Qiyue Zhang, Jing Wang, Yingli Liu, Yunying Zhao, Yu Deng
Summary: In order to reduce reliance on petroleum, utilizing industrial microorganisms for the biosynthesis of important chemicals from simple substrates has become a focus of interest. This review explores strategies to increase intracellular malonyl-CoA levels and advances in metabolic engineering for directing malonyl-CoA to desired derivatives, including strengthening supply, reducing consumption, and controlling levels effectively. These strategies offer new insights for enhancing malonyl-CoA derivative synthesis in microorganisms.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yongjie Liu, Zihan Liu, Huishu Zhang, Shaohua Yuan, Yanmei Li, Tianbao Zhang, Jianfang Bai, Liping Zhang
Summary: This study systematically analyzed the wheat LACS gene family and identified 30 putative TaLACS genes. It was found that 22 genes were expressed in wheat anthers, and a gene regulatory network related to male fertility was discovered.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Veronika Mater, Sabine Eisner, Cornelia Seidel, Dirk Schneider
Summary: Tuberculosis, a common infectious disease, infects about one-fourth of the global population. The causative agent, Mycobacterium tuberculosis, can enter a dormant state and display antibiotic resistance. The study investigates the membrane association and substrate specificity of MbFACL6, a potential target for treating resistant strains. MbFACL6 is found to be a peripheral membrane-attached protein and shows a broad spectrum of substrate tolerance.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Review
Peripheral Vascular Disease
Motohiro Sekiya, Kenta Kainoh, Kenji Saito, Daichi Yamazaki, Tomomi Tsuyuzaki, Wanpei Chen, Yuto Kobari, Ayumi Nakata, Haruka Babe, Hitoshi Shimano
Summary: CtBP2 serves as a critical metabolite sensor and plays a key role in maintaining metabolic homeostasis. It can sense various metabolites and is activated to protect against metabolic disturbances in healthy individuals, while inactivation of CtBP2 contributes to the pathogenesis of obesity.
JOURNAL OF ATHEROSCLEROSIS AND THROMBOSIS
(2023)
Article
Biochemistry & Molecular Biology
Runyi Huang, Wenli Yu, Rongzhen Zhang, Yan Xu
Summary: This study developed an efficient method for producing malonyl-CoA by screening the ACS gene from Streptomyces sp. The newly characterized ACS enzyme showed high conversion rate and yield of malonyl-CoA under optimized conditions.
PROCESS BIOCHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Yuyang Liu, Kivanc Birsoy
Summary: Mitochondria are organelles with their own genomes and unique biochemical reaction networks. They must maintain a specific biochemical environment and coordinate with the host cell's metabolic networks. This coordination involves sensing and controlling metabolites and responding to metabolic stresses. Dysfunction in the regulatory circuits of mitochondrial metabolic sensing and control can lead to metabolic disorders with clinical manifestations. This review discusses the logic and molecular basis of metabolic sensing and control in mitochondria, highlighting gaps in current knowledge that can potentially be addressed with emerging technologies.
Review
Microbiology
John E. Cronan
Summary: This review provides an update on the research of Escherichia coli acetyl-CoA carboxylase (ACC), focusing on the structures, stoichiometry, regulation of ACC activity and expression.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2021)