期刊
LIMNOLOGY AND OCEANOGRAPHY
卷 55, 期 3, 页码 1414-1425出版社
WILEY
DOI: 10.4319/lo.2010.55.3.1414
关键词
-
资金
- Gordon and Betty Moore Foundation
- National Science Foundation
Alkaline phosphatase (AP) activity (APA) was measured at several stations in the North Pacific Subtropical Gyre in July 2008, and in a series of nutrient addition experiments: nitrate plus ammonium (+N) or phosphate (+P), to study APA regulation and to evaluate the capacity of picoplankton organisms (i.e., in the 0.2-2-mu m size range) to access the AP-hydrolyzable fraction of dissolved organic phosphorus (DOP). The data indicated a primary limitation of the biomass by nitrogen. Both total (measured with a soluble DOP analog) and cell-specific (measured with the enzyme-labeled fluorescence [ ELF] phosphate cell labeling method) APA were enhanced in the +N samples and reduced in the +P samples, suggesting that DOP is an important resource for picoplankton nutrition. Cell-free APA represented > 65% of the APA in all samples, but its contribution to total APA significantly decreased in the +N treatment as microbial biomass increased. In the +N treatment, < 5% and up to 96% of the cells in the heterotrophic bacteria-enriched and picophytoplankton-enriched fractions, respectively, were ELF-alcohol-labeled after 5 d. Following N enrichment, the microbial assemblage shifted from cell-free phosphatase dominated under N limitation and P stress (i.e., physiological response) to picophytoplankton-based phosphatase dominated under P limitation (i.e., production or growth rate limitation). If, as predicted, the ocean evolves towards P limitation, DOP availability would become of major importance to sustain productivity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据