Review
Biochemistry & Molecular Biology
Raquel Gomez-Sintes, Esperanza Arias
Summary: Chaperone-mediated autophagy (CMA) is a proteolytic process that selectively degrades intracellular proteins inside lysosomes, participating in the modulation of specific regulatory proteins. Impairment of CMA function may contribute to the pathogenesis of neurodegenerative diseases and cancer, making it a potential therapeutic target for these conditions. Recent evidence has revealed the important and complex role of CMA in cancer biology, with implications for tumor development and the response to cancer therapeutics.
MOLECULAR ASPECTS OF MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Thibault Courtellemont, Maria Giovanna De Leo, Navin Gopaldass, Andreas Mayer
Summary: Endo-lysosomal compartments exchange proteins through different processes, including fusion, fission, and endosomal transport carriers. The membrane fission events that occur during these processes are not well understood. This study identifies the CROP complex as a factor that plays a role in membrane fission. The CROP complex consists of members from two protein families and enhances the membrane fission activity of a specific protein. Disrupting the CROP complex prevents fragmentation of lysosome-like structures in yeast and impairs cargo export in mammalian endosomes.
Article
Chemistry, Analytical
Kun Zhang, Guowei Ding, Fengqing Gai, Yafang Zhang, Xiaoni Wang, Zhiming Gou, Yujing Zuo
Summary: Autophagy is crucial for cellular health, and its dysfunction is associated with various conditions. The development of a polysiloxane-based probe that targets lysosomes and responds to pH changes offers a valuable tool for visualizing and studying autophagic processes. This innovative probe provides insights into the mechanisms of autophagy and potential therapeutic strategies for related diseases.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Peripheral Vascular Disease
Shibin Cheng, Zheping Huang, Sayani Banerjee, Sukanta Jash, Joel N. Buxbaum, Surendra Sharma
Summary: This study demonstrates the importance of protein aggregation in the pathophysiology of preeclampsia. The aggregation of TTR protein is found to be associated with endoplasmic reticulum stress and apoptosis. Furthermore, the use of transgenic mice provides further evidence of the link between TTR aggregation and preeclampsia-like features.
Review
Biochemistry & Molecular Biology
Francesco Agostini, Marco Bisaglia, Nicoletta Plotegher
Summary: Reactive oxygen species (ROS) generated from incomplete oxygen reduction can interact with and influence the function of various targets, including DNA, lipids, and proteins. AMP-activated protein kinase (AMPK), known as a major sensor of intracellular energy status, has been shown to play a crucial role in regulating cellular processes such as autophagy and lysosomal function. Through its modulation, AMPK can participate in the crosstalk between mitochondria and lysosomes by perceiving signals related to mitochondrial dynamics and transducing them to lysosomes, thereby impacting autophagic flux. Future studies should focus on the specific contribution of different AMPK subpopulations to the autophagic pathway, considering the tissue-specific regulation and localization of AMPK.
Article
Clinical Neurology
Chiara Vantaggiato, Genny Orso, Giulia Guarato, Francesca Brivio, Barbara Napoli, Elena Panzeri, Simona Masotti, Filippo Maria Santorelli, Maria Lamprou, Sentiljana Gumeni, Emilio Clementi, Maria Teresa Bassi
Summary: SPG15 is a hereditary spastic paraplegia subtype caused by mutations in the ZFYVE26 gene. Mutations in this gene lead to defects in autophagic lysosome reformation, resulting in symptoms such as enlarged lysosomes and accumulation of autophagosomes. Currently, the only available treatments are symptomatic and rehabilitative therapies.
Article
Biochemistry & Molecular Biology
Zsolt Lakatos, Peter Benko, Gabor Juhasz, Peter Lorincz
Summary: The study identified Drosophila Rab39 as a negative regulator of lysosomal clearance during both endocytosis and autophagy, providing valuable insights into potential cell biological mechanisms mediated by these proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Clinical Neurology
Yueyang Liu, Xiaohang Che, Haotian Zhang, Xiaoxiao Fu, Yang Yao, Jun Luo, Yu Yang, Ruiping Cai, Xiangnan Yu, Jingyu Yang, Ming-Sheng Zhou
Summary: The study demonstrates that CAPN1 activation impairs lysosomal function and suppresses autophagosome formation, leading to accumulation of substrates and aggravating neuronal cell damage in cerebral ischemia. The findings highlight the crucial role of CAPN1 in regulating autophagy-lysosomal pathway defects and neuronal damage in the context of cerebral ischemia.
Article
Hematology
Wenbo Qu, Xin Zhou, Xinjian Jiang, Xianwei Xie, Kaijian Xu, Xia Gu, Ruisi Na, Minghui Piao, Xiangwen Xi, Na Sun, Xueyu Wang, Xiang Peng, Junyan Xu, Jiangtian Tian, Jian Zhang, Junli Guo, Maomao Zhang, Yao Zhang, Zhenwei Pan, Kun Wang, Bo Yu, Bin Sun, Shuijie Li, Jinwei Tian
Summary: This study reveals the essential role of Gpr137b-ps in the regulation of macrophage autophagy and its impact on the progression of advanced atherosclerosis. Gpr137b-ps impairs the interaction between HSC70 and G3BP, leading to dysregulation of amino acid-mTORC1-autophagy signaling. These findings offer a potential therapeutic direction for advanced atherosclerosis.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Katarzyna M. Grochowska, Maria Andres-Alonso, Anna Karpova, Michael R. Kreutz
Summary: Synaptic function relies on the constant supply and removal of neuronal membranes. Neuronal protein transport faces challenges due to the complexity of neurons, but local micro-secretory systems have evolved to meet the requirements of neuronal membrane protein proteostasis. However, our knowledge of neuronal protein synthesis, trafficking, replacement, and degradation is limited.
Article
Cell Biology
Sierra B. Swords, Nuo Jia, Anne Norris, Jil Modi, Qian Cai, Barth D. Grant
Summary: This study reveals the critical role of RME-8/DNAJC13 protein in maintaining lysosomal capacity and autophagy, particularly in neurons. Loss of RME-8/DNAJC13 leads to abnormal accumulation of autolysosomal tubules in both C. elegans and primary mouse cortical neurons, and affects the process of autophagy. These findings provide insights into the potential impact of RME-8/DNAJC13 on neuronal health and the progression of neurodegenerative diseases.
Article
Immunology
Maud Wilhelm, Srinivasa Reddy Bonam, Nicolas Schall, Mykolas Bendorius, Anne-Sophie Korganow, Catherine Lumbroso, Sylviane Muller
Summary: A new class of autoantibodies targeting the C-terminus of LAMP2A, a receptor involved in chaperone-mediated autophagy, has been discovered. These autoantibodies may affect the autophagy process, which is abnormally upregulated in lupus. The findings support a novel connection between autophagy dysregulation, autoimmune processes, and pathophysiology in lupus.
JOURNAL OF AUTOIMMUNITY
(2021)
Article
Biochemistry & Molecular Biology
Jose A. Martina, Eutteum Jeong, Rosa Puertollano
Summary: TFEB-S401 phosphorylation is linked to the transcriptional control of monocyte differentiation.
Review
Cell Biology
Ruoxi Zhang, Rui Kang, Daniel J. Klionsky, Daolin Tang
Summary: Ion exchange between intracellular and extracellular spaces is crucial for cell metabolism and signal transduction. Autophagy, as a lysosomal-dependent degradation pathway, regulates cellular homeostasis through recycling of components and is influenced by ion channels and transporters. ATG proteins are central to the formation of autophagy-related structures, while the regulation of autophagy in mammalian cells involves ion channels and transporters.
Article
Chemistry, Analytical
Yu-Fang Wei, Xiao-Feng Weng, Xin-Long Sha, Ru Sun, Yu-Jie Xu, Jian-Feng Ge
Summary: This study reported a novel fluorescent probe for simultaneous detection of viscosity fluctuation in mitochondria and lysosomes, showing great potential in cell experiments. The probe exhibited stronger fluorescence in cancer cells at low concentrations and could be used for early diagnosis of related diseases.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Letter
Pharmacology & Pharmacy
Ilaria Campesi, Andrea Montella, Flavia Franconi
PHARMACOLOGICAL RESEARCH
(2021)
Review
Biology
Ilaria Campesi, Flavia Franconi, Andrea Montella, Salvatore Dessole, Giampiero Capobianco
Summary: Biological differences between sexes start in utero and continue on to fetal programming, yet there is a lack of stratification by sex in many biomedical fields. The sex of donors in biological research must be known, as variations in cells and cellular components can be important for pharmacological studies. This review highlights the importance of considering sex differences in research, particularly in the umbilical cord and fetal programming, in order to promote innovative research.
Article
Cell Biology
Ilaria Campesi, Andrea Montella, Flavia Franconi
Summary: Monocytes play a critical role in inflammation and immune response, and their activity is sex-dependent. The study found that LPS treatment led to qualitative and quantitative sex differences in monocytes, with differences in autophagy, TNF-alpha release, chemotaxis, and estrogen receptor expression between male and female cells. These results indicate that sex plays a significant role in regulating monocytes' functions in a cell- and parameter-specific manner.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Sport Sciences
Lucia Cugusi, Andrea Manca, Ernest Fischbach, Claudio Secci, Marco Bergamin, Stefano Gobbo, Andrea DI Blasio, Andrea Montella, Pasquale Bandiera, Franca Deriu
Summary: This retrospective survey analyzed the prevalence of low back pain (LBP) among Italian adolescent soccer players and identified potential risk factors. The results showed that more than half of the players had experienced LBP in their lives. Players with LBP were found to be taller, heavier, and have a higher BMI. Strikers aged 14-15 years displayed higher LBP scores compared to other roles. Strikers were also found to be at a higher risk of LBP compared to midfielders and goalkeepers, but not defenders. Within the 14-15 age group, strikers were the most exposed to LBP risk.
JOURNAL OF SPORTS MEDICINE AND PHYSICAL FITNESS
(2022)
Review
Endocrinology & Metabolism
Michele Costanzo, Marianna Caterino, Giovanni Sotgiu, Margherita Ruoppolo, Flavia Franconi, Ilaria Campesi
Summary: This review focused on sex-related metabolic differences by analyzing metabolomics studies on healthy populations, extracting shared and distinct features between males and females in the metabolome of common biological fluids, and considering the influence of age and the interaction between sex and age.
BIOLOGY OF SEX DIFFERENCES
(2022)
Article
Pharmacology & Pharmacy
Ilaria Campesi, Andrea Montella, Giovanni Sotgiu, Laura Saderi, Giancarlo Tonolo, Giuseppe Seghieri, Flavia Franconi
Summary: This study investigates the influence of sex, smoking, and combined oral contraceptives (COC) on atherogenic indexes. The results show that traditional lipid parameters are lower in men but higher in COC users. Smoking reduces sexually divergent parameters and narrows the differences between COC-free women and COC users. In women, the use of COC reveals two different cohorts. These findings highlight the importance of considering sex, smoking, and COC together in medical studies.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ilaria Campesi, Antonio Brunetti, Giampiero Capobianco, Adriana Galistu, Andrea Montella, Francesca Ieri, Flavia Franconi
Summary: In this study, the researchers investigated the response of male and female human umbilical vein endothelial cells to ionizing radiation and analyzed the influence of antioxidants on sex-dependent differences. The results showed that autophagy was higher in female cells in the absence of radiation. Radiation reduced cell viability and migration in a sex-independent manner, but antioxidants protected the cells and promoted wound closure.
Article
Medicine, General & Internal
Giuseppe Seghieri, Elisa Gualdani, Piergiorgio Francia, Ilaria Campesi, Flavia Franconi, Graziano Di Cianni, Paolo Francesconi
Summary: The aim of this study was to examine gender differences in mortality risk among patients with diabetic foot disease (DFD). Using data from Tuscany, Italy, the study found that women with DFD had a lower survival probability compared to men. After initial DFD hospitalizations, men had a significantly higher mortality risk of 18% compared to women.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Flavia Franconi, Giampiero Capobianco, Giuseppe Diana, Valeria Lodde, Alberto De Donno, Maria Laura Idda, Andrea Montella, Ilaria Campesi
Summary: This study investigates the sex-specific effects of Ang II on key processes such as endothelial dysfunction, angiotensin II, and autophagy in HUVECs. The results show that Ang II affects the expression of genes, proteins, and miRNAs in a sex-dependent manner. These findings contribute to a better understanding of the mechanisms underlying sex differences in endothelial dysfunction and provide valuable insights for the development of innovative biomarkers and personalized therapeutic approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ilaria Campesi, Giampiero Capobianco, Antonella Cano, Valeria Lodde, Sara Cruciani, Margherita Maioli, Giovanni Sotgiu, Maria Laura Idda, Mariangela Valentina Puci, Margherita Ruoppolo, Michele Costanzo, Marianna Caterino, Francesca Cambosu, Andrea Montella, Flavia Franconi
Summary: This study found sex-related differences in amniotic fluid and amniotic fluid cells. Inflammation markers and certain metabolites were higher in female amniotic fluid, while other metabolites were higher in male amniotic fluid. Female amniotic fluid cells expressed more estrogen receptors and proteins associated with autophagy, while male amniotic fluid cells expressed more proteins associated with lysosomes.
Article
Biochemistry & Molecular Biology
Rossana Migheli, Giada Lostia, Grazia Galleri, Gaia Rocchitta, Pier Andrea Serra, Ilaria Campesi, Valentina Bassareo, Elio Acquas, Alessandra T. Peana
Summary: This study reveals that Naloxone (NLX) has a protective effect against oxidative stress induced by hydrogen peroxide (H2O2) in PC12 cells. NLX reduces intracellular reactive oxygen species (ROS) production, apoptosis levels, and prevents G2/M phase cell cycle distribution and cellular damage. Electrochemical experiments further confirm the antioxidant properties of NLX.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2023)
Article
Education & Educational Research
Maria Alessandra Sotgiu, Pasquale Bandiera, Vittorio Mazzarello, Laura Saderi, Andrea Montella, Bernard John Moxham
Summary: This study is the first to assess medical students' perceptions of the value of Near Peer Teaching (NPT) for a course in human organ histology. The results showed that the majority of students rated the support of NPT tutors as excellent or good, and most students agreed that NPT should be introduced officially into the medical curriculum. These findings suggest that NPT could be a valuable instrument for the understanding of histological concepts.
EDUCATION SCIENCES
(2022)
Meeting Abstract
Anatomy & Morphology
Alessandro Riva, Marcello Trucas, Francesco Loy, Alessio Pirino, Andrea Montella
JOURNAL OF ANATOMY
(2022)
Article
Medicine, Research & Experimental
Yuwen Zhu, Yan Guo, Yujia Xue, Anqi Zhou, Ying Chen, Yifei Chen, Xiulian Miao, Fangqiao Lv
Summary: BRG1 plays an important role in HSC-myofibroblast transition and targeting it could be a reasonable strategy for liver fibrosis intervention.
Article
Medicine, Research & Experimental
Liu Ye, Beibei Liu, Jingling Huang, Xiaolin Zhao, Yuan Wang, Yungen Xu, Shuping Wang
Summary: Doublecortin-like kinase 1 (DCLK1) is a significant prooncogenic factor that is strongly associated with the malignant progression and clinical prognosis of various cancers. DCLK1 plays important roles in stem cell marker regulation, tumor cell reprogramming, and immune evasion. However, the exact biological functions of DCLK1, especially the disparities between its alpha- and beta-form transcripts in cancer progression, remain ambiguous.
Article
Medicine, Research & Experimental
Jiahui Yang, Xiaoyu Chen, Tianjing Liu, Yongyan Shi
Summary: This article reviews the role of bile acids in necrotizing enterocolitis (NEC) and their potential therapeutic value. The dysregulation of bile acids is associated with intestinal injury, and inflammatory factors in the liver also play a crucial role in regulating bile acid transport. The bile acid metabolic pathway is important for regulating intestinal microbiota, cell proliferation, and barrier protection.
Review
Medicine, Research & Experimental
Zhenzheng Zhu, Yuemiao Xu, Yuwei Xia, Xinru Jia, Yixin Chen, Yuyue Liu, Leyin Zhang, Hui Chai, Leitao Sun
Summary: Bile acid, as the final product of cholesterol breakdown, plays a complex regulatory and signaling role in human metabolism. Research suggests that it has the potential to enhance metabolism and regulate chronic metabolic diseases through various pathways. The interaction between bile acid and gut microbiota is also of great significance.
Article
Medicine, Research & Experimental
Xin He, Hong-Xu Zhou, Xian Fu, Kai-Di Ni, Ai-Zhi Lin, Ling-Tong Zhang, Hou-Hua Yin, Qing Jiang, Xue Zhou, Yi-Wen Meng, Jun-Yan Liu
Summary: DON exposure causes an increase in deoxycholic acid (DCA), which contributes to intestinal injury. DCA may be a potential therapeutic target for DON enterotoxicity.
Article
Medicine, Research & Experimental
Zhitao Wang, Heng Ma, Abdul Nasir, Sufang Liu, Zhisong Li, Feng Tao, Qian Bai
Summary: This study reveals the involvement of TET1-mediated epigenetic regulation in chronic TMJ pain through trigeminal TNF alpha signaling.
Article
Medicine, Research & Experimental
Lu Yu, Hao Ran, Yaru Lu, Qian Ma, Huan Huang, Weibin Liu
Summary: This study found that the HIF-1 alpha inhibitor BAY 87-2243 can alleviate the symptoms of the Experimental Autoimmune Myasthenia Gravis (EAMG) inflammation model. BAY 87-2243 can restore the balance of CD4(+)T cell subsets, reduce the production of pro-inflammatory cytokines, and act as both an immune imbalance regulator and anti-inflammatory.
Article
Medicine, Research & Experimental
Alex Peralvarez-Marin, Montse Sole, Judith Serrano, Alice Taddeucci, Belen Perez, Clara Penas, Gemma Manich, Marcel Jimenez, Pilar D'Ocon, Francesc Jimenez-Altayo
Summary: This study provides the first evidence that TRPV2 channels may modulate vascular tone by balancing opposing inputs from the endothelium and smooth muscle, leading to net vasodilation. The amplification of TRPV2 channel-induced activity by NO emphasizes the pathophysiological relevance of these findings.
Article
Medicine, Research & Experimental
Amin Ullah, Jing Zhao, Jiakun Li, Rajeev K. Singla, Bairong Shen
Summary: Gastric cancer is the fifth-most prevalent and second-most deadly cancer worldwide. Late onset of symptoms makes early detection important. CXC chemokines play an important role in the pathological process of gastric cancer, but their exact role in diagnosis and prognosis is not fully understood. Inhibiting CXC chemokines shows promise as a targeted therapy.
Article
Medicine, Research & Experimental
Menna S. Zeyada, Salma M. Eraky, Mamdouh M. El-Shishtawy
Summary: The current study demonstrates the prophylactic and antifibrotic effects of Trig against BLM-induced PF by targeting multiple signaling pathways. The combination of Trig and Pirf may be a promising approach to enhance Pirf's anti-fibrotic effect.