Article
Environmental Sciences
Raquel Chamorro-Garcia, Nathalie Poupin, Marie Tremblay-Franco, Cecile Canlet, Riann Egusquiza, Roselyne Gautier, Isabelle Jouanin, Bassem M. Shoucri, Bruce Blumberg, Daniel Zalko
Summary: Metabolomics confirmed transgenerational obesogenic effects of environmentally relevant doses of TBT in male mice, while also showing impaired liver biotransformation capacity in females. This study demonstrates the transgenerational effects of EDC exposure and highlights the potential use of metabolomic fingerprints in identifying individuals susceptible to adverse health effects from ancestral exposure to obesogens.
ENVIRONMENT INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Hyeon-Young Kim, Hyeon-Seong Lee, In-Hyeon Kim, Youngbae Kim, Moongi Ji, Songjin Oh, Doo-Young Kim, Wonjae Lee, Sung-Hwan Kim, Man-Jeong Paik
Summary: This study evaluated the metabolic mechanisms and potential biomarkers of COPD through the integrated analysis and receiver operating characteristic (ROC) analysis of metabolic changes in lung, plasma, and urine. The results proposed potential biomarkers in plasma and urine that reflect altered lung metabolism in COPD. Understanding these integrative mechanisms provides new insights into the diagnosis, treatment, and severity assessment of COPD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Environmental
Hsuan-Cheng Lu, Anupama Kumar, Steven D. Melvin, Shima Ziajahromi, Peta A. Neale, Frederic D. L. Leusch
Summary: In this study, the effects of irregular shaped polyethylene microplastics (MPs) on Chironomus tepperi were investigated. The parental generation showed decreased survival and emergence rates at high MP concentrations, while growth-related endpoints were only affected at the highest concentration. Metabolite analysis revealed lower abundances of amino acid and energy metabolism-related metabolites at the highest exposure concentration, suggesting an impact on bioenergetics. However, no significant effects were observed in the continuous exposure of the first filial generation.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Agriculture, Dairy & Animal Science
H. Boudra, P. Noziere, G. Cantalapiedra-Hijar, M. Traikia, J-F. Martin, M. Petera, M. Lagree, M. Doreau, D. P. Morgavi
Summary: The study found that spot urine collection is a practical alternative for metabolomic studies in lactating cows. While different sampling techniques may affect the abundance of detected metabolites, spot urine samples are equally capable of showing differences in urine metabolome compared to total collection samples.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Environmental Sciences
Pan Deng, Jerika Durham, Jinpeng Liu, Xiaofei Zhang, Chi Wang, Dong Li, Taesik Gwag, Murong Ma, Bernhard Hennig
Summary: This study found that consumption of soluble fibers could mitigate the adverse effects of PFOS on liver metabolism and gut microbiota. Through multi-omics analysis, the study provides new insights into the interaction between nutrients and environmental pollutants.
ENVIRONMENTAL HEALTH PERSPECTIVES
(2022)
Article
Cell Biology
Sehoon Park, Jueun Lee, Seung Hee Yang, Hajeong Lee, Joo Young Kim, Minkyoung Park, Kyu Hong Kim, Jong Joo Moon, Semin Cho, Soojin Lee, Yaerim Kim, Jung Pyo Lee, Chang Wook Jeong, Cheol Kwak, Kwon Wook Joo, Chun Soo Lim, Yon Su Kim, Geum-Sook Hwang, Dong Ki Kim
Summary: The study identified urinary glycine as a potential biomarker for the diagnosis and prognosis of IgAN. Higher levels of urinary glycine were associated with a lower risk of kidney function decline in IgAN patients. Experimental validation in human kidney tubular epithelial cells showed that glycine could ameliorate inflammatory signals induced by tumor necrosis factor-alpha.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Genetics & Heredity
Zhi-e Fang, Chunyu Wang, Ming Niu, Tingting Liu, Lutong Ren, Qiang Li, Zhiyong Li, Ziying Wei, Li Lin, Wenqing Mu, Yuan Gao, Xiaohe Xiao, Zhaofang Bai
Summary: This study investigates the impact of aristolochic acid on liver injury in mice of different ages and finds that neonatal mice are more susceptible to liver injury compared to adult mice. Metabolomics and transcriptomics analyses reveal that AAI disrupts several metabolic pathways and leads to liver injury in mice.
FRONTIERS IN GENETICS
(2022)
Article
Multidisciplinary Sciences
Brij Bhushan, Deepti Upadhyay, Uma Sharma, Naranamangalam Jagannathan, Shashi Bala Singh, Lilly Ganju
Summary: The harsh environment of Antarctica has a significant impact on human physiology and metabolic processes, with notable changes in metabolite levels observed in urine samples collected post-Antarctic expedition. Metabolic adaptation to Antarctic environmental stressors may highlight short-term physiological effects and provide valuable information for expedition management programs in Antarctica.
Article
Pharmacology & Pharmacy
Taha Messelmani, Anne Le Goff, Fabrice Soncin, Francoise Gilard, Zied Souguir, Nathalie Maubon, Bertrand Gakiere, Cecile Legallais, Eric Leclerc, Rachid Jellali
Summary: Organ-on-chip technology is a promising in vitro approach for studying responses to drug exposure. This study used a metabolomic investigation to analyze a coculture of liver sinusoidal endothelial cells (LSECs) with hepatocytes using advanced organ-on-chip technology. The results demonstrated the potential of a metabolomic-on-chip strategy for pharmaco-metabolomic applications.
Article
Biochemical Research Methods
Hongmei Tang, Tingting Peng, Xubo Yang, Liru Liu, Yunxian Xu, Yiting Zhao, Shiya Huang, Chaoqiong Fu, Yuan Huang, Hongyu Zhou, Jinling Li, Lu He, Wenda Wang, Huiran Niu, Kaishou Xu
Summary: In this study, significant dynamic changes of plasma metabolites were observed in children with cerebral palsy (CP) after botulinum neurotoxin A (BoNT-A) injections. Metabolic pathways associated with energy expenditure and glycine, serine, and threonine metabolism, as well as cysteine and methionine metabolism, might be related to the duration of effect of BoNT-A in children with CP.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Engineering, Biomedical
Camila Bussola Tovani, Thibaut Divoux, Sebastien Manneville, Thierry Azais, Guillaume Laurent, Marta de Frutos, Alexandre Gloter, Pietro Ciancaglini, Ana P. Ramos, Nadine Nassif
Summary: This study investigates the physiological and pathological effects of strontium (Sr2 +) on bone biomineralization. The results show that excessive levels of Sr2 + destabilize the mineral and collagen fibril assembly, leading to impaired cellular activity and correlating with bone-weakening disorders.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Medicinal
Yahao Gao, Di Jiang, Changshui Wang, Gang An, Li Zhu, Changmeng Cui
Summary: This study comprehensively evaluated the toxicity of sodium valproate (VPA) using metabolomics, and found that VPA-induced toxicity is closely related to 12 key pathways including oxidative stress, inflammation, amino acid metabolism, lipid metabolism, and energy disorder.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2022)
Article
Biotechnology & Applied Microbiology
Fionn O. Fearghail, Patrice Behan, Niklas Engstrom, Nathalie Scheers
Summary: The developed LCMS metabolomic workflow allows for comparison of metabolic patterns from human intestinal cells exposed to different treatments, with the aim of identifying the most bioactive treatment. Changes in cellular metabolic patterns were found to be useful predictors of bioactivity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Food Science & Technology
Xiaoli Guo, Cheng Cheng, Lin Chen, Changsong Cao, Dongbei Li, Ruihua Fan, Xudong Wei
Summary: Nanoplastics are a significant environmental concern with potential harm to organisms. The effect of polystyrene nanoplastics (PSNPs) on human CD34+ hematopoietic stem/progenitor cells (HSPCs) and its underlying mechanism were investigated in this study. PSNPs were found to be taken up by cells, resulting in decreased cell viability and cell membrane damage. PSNPs exposure also inhibited HSPC proliferation and differentiation. Metabolomics analysis revealed disruptions in various metabolic pathways, including the citrate cycle, after PSNPs exposure. These findings contribute to understanding the toxic mechanisms and identifying potential metabolism-related biomarkers of hematopoietic damage caused by nanoplastics exposure.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Oncology
Ming Yang, Xiaoyan Liu, Xiaoyue Tang, Wei Sun, Zhigang Ji
Summary: Urine metabolomics is a promising technique in the liquid biopsy of urothelial cancer (UC). In this study, urine samples from UTUCs, BCas, and HCs were analyzed using LC-HRMS, and differential metabolites and disturbed metabolism pathways were explored. The combination of urine metabolomics and transcriptomics identified potential aberrant genes in BCa metabolism.
FRONTIERS IN ONCOLOGY
(2023)
Article
Biology
Rebecca C. Schugar, Christy M. Gliniak, Lucas J. Osborn, William Massey, Naseer Sangwan, Anthony Horak, Rakhee Banerjee, Danny Orabi, Robert N. Helsley, Amanda L. Brown, Amy Burrows, Chelsea Finney, Kevin K. Fung, Frederick M. Allen, Daniel Ferguson, Anthony D. Gromovsky, Chase Neumann, Kendall Cook, Amy McMillan, Jennifer A. Buffa, James T. Anderson, Margarete Mehrabian, Maryam Goudarzi, Belinda Willard, Tytus D. Mak, Andrew R. Armstrong, Garth Swanson, Ali Keshavarzian, Jose Carlos Garcia-Garcia, Zeneng Wang, Aldons J. Lusis, Stanley L. Hazen, Jonathan Mark Brown
Summary: This study demonstrates the potential of gut microbe-targeted enzyme inhibitors as anti-obesity therapeutics by improving metabolic disturbances and energy expenditure. It underscores the relationship between microbiome and host metabolism.
Article
Multidisciplinary Sciences
Kamendra Kumar, Kamal Datta, Albert J. Fornace, Shubhankar Suman
Summary: This study investigated the effects of proton and high-LET heavy-ion radiation on bone marrow cells and found that exposure to both types of radiation led to increased cell senescence and pro-osteoclastogenic activity. The effects were more pronounced with Fe-56 ions compared to protons. This study has important implications for understanding bone degeneration in deep-space astronauts and patients undergoing radiotherapy.
Article
Biology
Evan L. Pannkuk, Evagelia C. Laiakis, Jerry Angdisen, Meth M. Jayatilake, Pelagie Ake, Lorreta Yun-Tien Lin, Heng-Hong Li, Albert J. Fornace
Summary: Several diagnostic biodosimetry tools are being developed for radiological/nuclear emergency responses. Correlating changes in non-invasive biofluid small-molecule signatures to tissue damage from ionizing radiation exposure shows promise for predictive biodosimetry models. Genotypic variation in the general population may affect model performance in dose reconstruction.
RADIATION RESEARCH
(2022)
Article
Biology
Constantinos G. Broustas, Sanjay Mukherjee, Evan L. Pannkuk, Evagelia C. Laiakis, Albert J. Fornace, Sally A. Amundson
Summary: Radiation biodosimetry based on transcriptomic analysis of peripheral blood is a valuable tool for detecting radiation exposure, but confounding factors may affect its predictive power. The p38 signaling pathway plays an important role in the response to radiation, and its attenuation may protect blood cells from radiation-induced damage.
RADIATION RESEARCH
(2022)
Article
Genetics & Heredity
Jonathan P. Jacobs, Maryam Goudarzi, Venu Lagishetty, Dalin Li, Tytus Mak, Maomeng Tong, Paul Ruegger, Talin Haritunians, Carol Landers, Philip Fleshner, Eric Vasiliauskas, Andrew Ippoliti, Gil Melmed, David Shih, Stephan Targan, James Borneman, Albert J. Fornace, Dermot P. B. McGovern, Jonathan Braun
Summary: Crohn's disease patients in endoscopic remission show differences in the microbiome compared to unaffected controls, influenced by inflammation, genetic risk, and disease phenotype. Microbial profiling during endoscopic remission can predict disease behavior and progression, offering insight into CD pathogenesis and potential prognostic biomarkers.
Article
Astronomy & Astrophysics
Kamendra Kumar, Bo-Hyun Moon, Kamal Datta, Albert J. Fornace Jr, Shubhankar Suman
Summary: This study examines the status of mammary cancer-associated preneoplasia markers in mice exposed to galactic cosmic radiation (GCR) and gamma-ray irradiation. The findings suggest that mice exposed to GCR have a higher risk of mammary cancer compared to those exposed to gamma-rays, as indicated by increased ductal outgrowth and cell proliferation in mammary tissues.
LIFE SCIENCES IN SPACE RESEARCH
(2023)
Review
Astronomy & Astrophysics
Janice L. Huff, Floriane Poignant, Shirin Rahmanian, Nafisah Khan, Eleanor A. Blakely, Richard A. Britten, Polly Chang, Albert J. Fornace, Megumi Hada, Amy Kronenberg, Ryan B. Norman, Zarana S. Patel, Jerry W. Shay, Michael M. Weil, Lisa C. Simonsen, Tony C. Slaba
Summary: For missions to the moon or Mars, space explorers will face a complex radiation field with various ion species and energies. The NASA Space Radiation Laboratory (NSRL) has developed an innovative galactic cosmic ray simulator (GCRsim) to simulate the space radiation environment and study biological risks. The GCRsim consists of 33 ion beams that simulate the primary and secondary GCR fields encountered in space. A recent virtual workshop assessed the status of the GCRsim, discussing its design and beam selection strategies. This information is important for advancements in space radiobiology.
LIFE SCIENCES IN SPACE RESEARCH
(2023)
Article
Biochemical Research Methods
Evan L. Pannkuk, Nicole A. S. -Y. Dorville, Shivani Bansal, Sunil Bansal, Yvonne A. Dzal, Quinn E. Fletcher, Kaleigh J. O. Norquay, Albert J. Fornace Jr, Craig K. R. Willis
Summary: This study investigates the lipidomic changes in bats with white-nose syndrome (WNS) and suggests that oxidative stress occurs in the early stages of WNS before fat depletion, but not inflammatory response. The study compared WNS-susceptible Myotis lucifugus to WNS-resistant Eptesicus fuscus and found altered splenic lipid levels only in M. lucifugus.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Santosh Kumar, Shubhankar Suman, Bo-Hyun Moon, Albert J. Fornace Jr, Kamal Datta
Summary: This study reveals that radiation exposure induces long-term oxidative stress in colonic epithelial cells, which is associated with colon carcinogenesis. The NADPH oxidase pathway may play a critical role in propagating this oxidative stress after radiation exposure.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Biochemical Research Methods
Lewis Y. Y. Geer, Joel Lapin, Douglas J. J. Slotta, Tytus D. D. Mak, Stephen E. E. Stein
Summary: By using a neural network to predict complete spectra, we created predicted spectral libraries that were used to rescore sequence search results, resulting in improved peptide identification accuracy and increased number of identifications, particularly for modified and phosphorylated peptides.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Oncology
Elaina Kwiatkowski, Shubhankar Suman, Bhaskar V. S. Kallakury, Kamal Datta, Albert J. Fornace Jr, Santosh Kumar
Summary: This study assessed the expression of multiple stem cell markers in premalignant tumors after low- and high-LET radiation. The results showed a correlation between increased levels of stemness markers and fi-catenin activation in premalignant tumors, particularly in tumors induced by high-LET radiation. The study highlights the complex relationship between radiation types and stem cell phenotypes, and their potential influence on carcinogenesis processes.
Review
Oncology
Kamendra Kumar, Santosh Kumar, Kamal Datta, Albert J. J. Fornace Jr, Shubhankar Suman
Summary: High-energy heavy ions can cause DNA damage, leading to cellular responses such as cell cycle arrest, cell death, and cellular senescence. Prolonged exposure to high-energy ions in space radiation increases the risk of gastrointestinal (GI) carcinogenesis. Alterations in DNA damage response (DDR) can result in gene mutations and pro-inflammatory, pro-oncogenic signaling, accelerating adenoma-to-carcinoma progression during radiation-induced GI cancer development.
Article
Medicine, Research & Experimental
Di Cui, Jianneng Li, Ziqi Zhu, Michael Berk, Aimalie Hardaway, Jeffrey McManus, Yoon-Mi Chung, Mohammad Alyamani, Shelley Valle, Ritika Tiwari, Bangmin Han, Maryam Goudarzi, Belinda Willard, Nima Sharifi
Summary: After androgen deprivation, prostate cancer frequently becomes castration resistant (CRPC), involving intratumoral androgen production. Cancer-associated fibroblasts (CAFs) increase 3 beta HSD1 expression and induce androgen synthesis, leading to CRPC.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Endocrinology & Metabolism
Aimalie L. Hardaway, Maryam Goudarzi, Michael Berk, Yoon-Mi Chung, Renliang Zhang, Jianneng Li, Eric Klein, Nima Sharifi
Summary: This study reveals that arachidonic acid and its metabolite 5-hydroxyeicosatetraenoic acid (5-HETE) regulate androgen metabolism. Arachidonic acid reduces androgens by inducing the expression of AKR1C2 and AKR1C3, and these effects require the expression of Nrf2.
Article
Medicine, Research & Experimental
Renxiang Chen, Yun-Tien Lin, Albert J. Fornace, Heng-Hong Li
Summary: The increasing number of compounds and chemicals that require safety assessments pose a serious challenge for regulatory agencies. In vitro screening using toxicogenomic biomarkers has been proposed as a solution, and a highly automated, multiplexed, and high-throughput genotoxicity testing assay has been developed to increase screening efficiency.
ALTEX-ALTERNATIVES TO ANIMAL EXPERIMENTATION
(2022)
