Article
Biochemistry & Molecular Biology
Anna Polz, Kamal Morshed, Robert Bibik, Bartlomiej Drop, Andrzej Drop, Malgorzata Polz-Dacewicz
Summary: This study aimed to investigate the importance of miR-31-5p and miR-let 7a in the pathogenesis of EBV-associated oropharyngeal cancer. The results showed a significantly increased serum level of miR-31-5p but decreased level of miR-let 7a in EBV positive oropharyngeal cancer patients. The study also found an association between the levels of these miRs and clinical stage, suggesting their potential as important biomarkers for the diagnosis of EBV-associated oropharyngeal cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Health Care Sciences & Services
Paola Fuso, Mariantonietta Di Salvatore, Concetta Santonocito, Donatella Guarino, Chiara Autilio, Antonino Mule, Damiano Arciuolo, Antonina Rinninella, Flavio Mignone, Matteo Ramundo, Brunella Di Stefano, Armando Orlandi, Ettore Capoluongo, Nicola Nicolotti, Gianluca Franceschini, Alejandro Martin Sanchez, Giampaolo Tortora, Giovanni Scambia, Carlo Barone, Alessandra Cassano
Summary: The up-regulation of miRNAs let-7a-5p, mirR-100-5p, miR-101-3p, and miR-199a-3p is associated with better event-free survival and overall survival in breast cancer patients, regardless of breast cancer subtypes.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Yu-Jen Chen, Chia-Tien Hsu, Shang-Feng Tsai, Cheng-Hsu Chen
Summary: Chronic allograft dysfunction (CAD) is a major condition affecting long-term kidney graft survival. In this study, the correlation between plasma miR-21 and the severity of CAD was investigated. RNA sequencing was performed and differentially expressed miRNAs in CAD were identified. MiR-21-5p, miR-20a-5p, and miR-101-3p were found to be associated with the TGF-beta pathway and CAD. These findings may lead to the development of early prediction miRNA biomarkers and therapeutic tools for CAD in the field of kidney transplantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Hallgeir Selven, Sigve Andersen, Mona Pedersen, Ana Paola Giometti Lombardi, Lill-Tove Rasmussen Busund, Thomas Karsten Kilvaer
Summary: This study investigated the prognostic impact of miR-17-5p and miR-20a-5p in colon cancer. The results showed that high expression of both miR-17-5p and miR-20a-5p is associated with a favorable prognosis and inhibits cell migration.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Sarah Hamdy Ahmed, Nancy A. Espinoza-Sanchez, Ahmed El-Damen, Sarah Atef Fahim, Mohamed A. Badawy, Burkhard Greve, Mohamed El-Shinawi, Martin Goette, Sherif Abdelaziz Ibrahim
Summary: Inflammatory breast cancer is a rare but aggressive form of breast cancer that can be diagnosed through miRNAs in small extracellular vesicles in the blood, with certain miRNAs found to be associated with the cancer. Analyzing these miRNAs can serve as promising diagnostic biomarkers for IBC.
Article
Multidisciplinary Sciences
Fengchang Bao, Lei Zhang, Xiaohang Pei, Cheng Lian, Yanhui Liu, Hongna Tan, Pingchong Lei
Summary: miR-20a-5p is significantly decreased in AML patients' bone marrow, correlated with risk status and poor survival prognosis, making it a potential therapeutic target for AML. Overexpression of miR-20a-5p suppresses AML cell proliferation and induces apoptosis, possibly through negative regulation of PPP6C.
Review
Cell Biology
Wei Huang, Xiaoyue Wu, Shuaixi Xiang, Mingxin Qiao, Xiao Cen, Xuefeng Pan, Xinqi Huang, Zhihe Zhao
Summary: MicroRNAs (miRNAs) are important non-coding RNA molecules that play a critical role in cancer diagnosis, prognosis, and treatment. miR-20a-5p has been found to have anti-cancer effects in various types of cancer, making it a significant biomarker for cancer diagnosis. This paper reviews and summarizes the expression differences of miR-20a-5p in cancer, the changes of miR-20a-5p networks, and the mechanism by which it regulates downstream gene expression. Based on these findings, miR-20a-5p is believed to be a promising and effective marker for cancer diagnosis, prognosis, and treatment.
CELL DEATH DISCOVERY
(2022)
Article
Cell Biology
Hongzhang Shen, Fuqiang Ye, Dongchao Xu, Liangliang Fang, Xiaofeng Zhang, Juanjuan Zhu
Summary: In this study, we found that the expression of MYEOV gene is associated with poor survival in patients with pancreatic ductal adenocarcinoma (PDAC) and is higher in carcinoma tissues. Elevated levels of MYEOV promote cell proliferation, invasion and migration. Further analysis revealed that MYEOV regulates the expression levels of miR-17-5p and miR-93-5p, and its association with MYC contributes to the progression of PDAC.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Yan Guo, Fen Du, Yi-Lan Tan, Jun Luo, Dan Xiong, Wei-Tao Song
Summary: miR-20a-5p and miR-17-5p inhibit cell proliferation and angiogenesis by regulating HIF-1α and VEGF in the retinal cells of OIR model mice. They bind to HIF-1α and VEGF at the 3'UTR and have a combined effect on their regulation, ultimately affecting angiogenesis associated with ROP.
BIOCHEMISTRY AND CELL BIOLOGY
(2021)
Article
Genetics & Heredity
Mayumi Ueta, Hiromi Nishigaki, Seitaro Komai, Katsura Mizushima, Risa Tamagawa-Mineoka, Yuji Naito, Norito Katoh, Chie Sotozono, Shigeru Kinoshita
Summary: In this study, comprehensive plasma miRNA analysis was performed to investigate the pathogenesis of atopic dermatitis (AD) with and without severe allergic conjunctival disease (ACD). Hsa-let-7a-5p was found to be significantly up-regulated in AD-ACD( + ) and AD-ACD( - ), and it may positively regulate important innate immune-related genes such as TLR3, RIG-I, and MDA5.
FRONTIERS IN GENETICS
(2023)
Article
Biochemistry & Molecular Biology
Madara Ratnadiwakara, Mohamed N. M. Bahrudeen, Erika Aikio, Piia Takabe, Rebekah M. Engel, Zileena Zahir, Thierry Jarde, Paul J. McMurrick, Helen E. Abud, Minna-Liisa Anko
Summary: This study reveals that Serine-arginine rich splicing factor 3 (SRSF3) plays a crucial role in the processing of miR-17-92 cluster miRNAs in pluripotent and cancer cells. SRSF3 binding to CNNC motifs downstream of Drosha cleavage sites is required for efficient processing of the cluster. The interaction between SRSF3 and miR-17-92 results in changes in RNA structure and an increase in miR-17 and miR-20a levels, promoting self-renewal and contributing to colorectal cancer pathogenesis.
Article
Biochemistry & Molecular Biology
Viviana Costa, Marcello De Fine, Valeria Carina, Alice Conigliaro, Lavinia Raimondi, Angela De Luca, Daniele Bellavia, Francesca Salamanna, Riccardo Alessandro, Giovanni Pignatti, Milena Fini, Gianluca Giavaresi
Summary: The research focuses on the role of miR-31-5p and miR-33a-5p in osteoarthritis progression, with identification of Specificity proteins (Sp1) as common miRNA targets through bioinformatic analysis. The study evaluates the possible mechanism of how these miRNAs regulate Sp1 to affect CX43 expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Dentistry, Oral Surgery & Medicine
Xuefeng Pan, Xinqi Huang, Bo Zhang, Fang Pei, Zhihe Zhao, Xiao Cen
Summary: The study found that miR-20a-5p inhibits the chondrogenic differentiation of hDPSCs by repressing the expression of SMAD6 and activating the NF-kappa B signaling pathway. Knocking down miR-20a-5p promotes cartilage regeneration in osteochondral defects.
Article
Oncology
Wenyan Liao, Jun He, Cyrollah Disoma, Yi Hu, Junhua Li, Guodong Chen, Ying Sheng, Xinyu Cai, Chuanfu Li, Kai Cheng, Chunfen Yang, Yongxi Qin, Dong Han, Wu Wen, Chengming Ding, Mujun Li
Summary: This study demonstrated that hsa_circ_0107593 has low expression in cervical cancer tissues and cell lines, negatively correlated with tumor characteristics, and inhibits cell proliferation, migration, and invasion. It may serve as a diagnostic biomarker due to its tumor-suppressing activity by sponging hsa-miR-20a-5p, hsa-miR-93-5p, and hsa-miR-106b-5p in cervical cancer.
FRONTIERS IN ONCOLOGY
(2021)
Article
Medicine, Research & Experimental
Yan Sweat, Ryan J. Ries, Mason Sweat, Dan Su, Fan Shao, Steven Eliason, Brad A. Amendt
Summary: Anaplastic thyroid cancer is a highly metastatic cancer expressing high levels of the miR-17-92 cluster. Inhibition of miR-17 unexpectedly promotes the expression of other oncogenic miRs within the cluster, leading to cancer progression. The study suggests a complex regulatory role of miR-17 in thyroid tumor growth.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Biochemistry & Molecular Biology
Panagiotis Tsiakanikas, Panagiotis G. Adamopoulos, Dimitra Tsirba, Pinelopi Artemaki, Iordanis N. Papadopoulos, Christos K. Kontos, Andreas Scorilas
Summary: This study investigated the prognostic utility of 5'-tiRNA-Pro(TGG) levels in colorectal cancer (CRC) and found that high levels were associated with poor survival rates, especially in patients with rectal cancer and moderately differentiated CRC. Importantly, the overexpression of 5'-tiRNA-Pro(TGG) was identified as an adverse prognostic factor independently predicting short-term relapse in CRC patients.
Article
Genetics & Heredity
Maria Papatsirou, Marios A. Diamantopoulos, Katerina Katsaraki, Dimitris Kletsas, Christos K. Kontos, Andreas Scorilas
Summary: This study identified nine novel circRNAs in breast cancer cell lines and predicted their potential involvement in tumor onset and progression based on their molecular properties.
Article
Biochemistry & Molecular Biology
Aimilia D. Sklirou, Despoina D. Gianniou, Paraskevi Karousi, Christina Cheimonidi, Georgia Papachristopoulou, Christos K. Kontos, Andreas Scorilas, Ioannis P. Trougakos
Summary: The expression of HSPB2 is significantly downregulated in breast tumors, and high HSPB2 mRNA expression is associated with poor disease-free survival and overall survival in breast cancer patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Genetics & Heredity
Pinelopi I. Artemaki, Christos K. Kontos
Article
Cell Biology
Maria Angeliki S. Pavlou, Kartikeya Singh, Srikanth Ravichandran, Rashi Halder, Nathalie Nicot, Cindy Birck, Luc Grandbarbe, Antonio del Sol, Alessandro Michelucci
Summary: Astrocytes, derived from neural stem cells (NSCs), are the predominant cell type in the central nervous system (CNS) and play important roles in brain development and function. Inflammation disrupts the normal differentiation process of NSCs into astrocytes, leading to abnormal astrocytic phenotypes. This study investigates the transcriptional and epigenetic changes underlying NSCs' differentiation into astrocytes in the presence of inflammation, and identifies key molecular regulators involved in this process.
Article
Genetics & Heredity
Paraskevi Karousi, Christos K. Kontos, Panagiota Papakotsi, Ioannis K. Kostakis, Alexios-Leandros Skaltsounis, Andreas Scorilas
Summary: This study found that oleuropein and oleocanthal can inhibit the proliferation of triple-negative breast cancer (TNBC) cells and significantly alter the expression profile of TNBC cells. These compounds may have therapeutic benefits for TNBC through their effects on multiple pathways associated with TNBC.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)
Article
Biochemistry & Molecular Biology
Christos K. Kontos, Paraskevi Karousi, Pinelopi I. Artemaki, Ahmed Abdelgawad, Aspasia Dimitriadou, Nikolaos P. Machairas, Diamantis C. Sideris, Vasiliki Pappa, Andreas Scorilas, Mona Batish, Sotirios G. Papageorgiou
Summary: In this study, we identified several novel circRNAs produced by BAX and BCL2L12, characterized by diverse exon structures. Interestingly, visualization of the most abundant circRNAs showed distinct intracellular localization. Additionally, a complex expression pattern of BAX and BCL2L12 circRNAs was revealed in CLL patients and non-leukemic blood donors, suggesting a multifaceted role in B-cell CLL.
Article
Medicine, General & Internal
Sotirios G. Papageorgiou, Ioanna Mavroeidi, Marios Kostakis, Aris Spathis, Danai Leventakou, Evangelia Kritikou, Nikolaos Oikonomopoulos, Chrysoula Kourkouti, Maria Krania, Anthi Bouchla, Thomas Thomopoulos, Zoi Tsakiraki, Konstantinos Markakis, Ioannis G. Panayiotides, Nikolaos Thomaidis, Vasiliki Pappa, Periklis G. Foukas, Melpomeni Peppa
Summary: Primary adrenal lymphoma (PAL) is a rare disease presenting as unilateral or bilateral rapidly growing adrenal masses. This study reports two cases of PAL presenting as adrenal incidentalomas (AIs) with autonomous cortisol production. Both patients showed symptoms of lumbar pain and were diagnosed with PAL through imaging and hormonal evaluation. They underwent biopsies that confirmed the presence of diffuse large B-cell lymphoma. Treatment was administered, but one patient died from infection shortly after. This study highlights the need for further analysis of PAL cases to understand its pathogenesis and course.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Christos K. Kontos, Despina Hadjichambi, Maria Papatsirou, Paraskevi Karousi, Spyridon Christodoulou, Diamantis C. Sideris, Andreas Scorilas
Summary: This study identifies 23 novel BOK circRNAs, which consist of both exonic and intronic regions of the BOK gene. These circRNAs could play a role in the molecular landscape and clinical investigation of ovarian and prostate cancer.
Article
Oncology
Katerina Katsaraki, Christos K. Kontos, Gerasimos Ardavanis-Loukeris, Alexandros A. Tzovaras, Diamantis C. Sideris, Andreas Scorilas
Summary: This study investigates the regulatory effect of proteasome inhibitors (PIs) on miRNA expression in breast cancer (BrCa) cell lines. The results show a high heterogeneity in miRNA levels among different cell lines and different treatment durations. Three miRNAs (miR-1-3p, miR-421-3p, and miR-765-3p) are identified as potential key players in BrCa. Various biological processes, molecular functions, and pathways are predicted to be affected.
CLINICAL & TRANSLATIONAL ONCOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Tasneem Abaza, Mostafa K. Abd El-Aziz, Kerolos Ashraf Daniel, Paraskevi Karousi, Maria Papatsirou, Sherif Ashraf Fahmy, Nadia M. Hamdy, Christos K. Kontos, Rana A. Youness
Summary: Hepatocellular carcinoma (HCC) is a deadly malignancy with limited treatment options and high recurrence rates. Recently, immunotherapeutic agents such as immune checkpoint inhibitors (ICIs) and circular RNAs (circRNAs) have shown promise in HCC treatment. CircRNAs can influence the tumor immune microenvironment and may impact the effectiveness of ICIs. Therefore, circRNAs have the potential to serve as biomarkers for diagnosis, prognosis, and targeted therapy in HCC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Genetics & Heredity
Paraskevi Karousi, Martina Samiotaki, Manousos Makridakis, Jerome Zoidakis, Diamantis C. Sideris, Andreas Scorilas, Thomas Carell, Christos K. Kontos
Summary: This study investigates the role of 3'-tRF-Cys(GCA) in gene expression regulation in HEK-293 cells and finds that it can regulate gene expression by affecting multiple cellular pathways. Additionally, the study identifies the direct interaction between 3'-tRF-Cys(GCA) and thymopoietin and its modulation of the protein's levels.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
P. I. Artemaki, A. Scorilas, M. Batish, C. K. Kontos
Meeting Abstract
Biochemistry & Molecular Biology
P. Karousi, A. Scorilas, D. C. Sideris, C. K. Kontos
Meeting Abstract
Biochemistry & Molecular Biology
P. I. Artemaki, P. Karousi, S. G. Papageorgiou, V. Pappa, A. Scorilas, C. K. Kontos
