Article
Biochemistry & Molecular Biology
Wojciech Gierlikowski, Katarzyna Broniarek, Lukasz Cheda, Zbigniew Rogulski, Marta Kotlarek-Lysakowska
Summary: In the context of papillary thyroid carcinoma, miR-181a-5p has been found to directly regulate the expression of SLC5A5, potentially reducing the efficacy of radioiodine treatment. This suggests that miR-181a-5p could serve as a promising target to enhance the efficacy of radioactive iodine therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Daniel G. Henriques, Renan Lyra Miranda, Romulo Sperduto Dezonne, Luiz Eduardo Wildemberg, Aline Helen da Silva Camacho, Leila Chimelli, Leandro Kasuki, Elisa B. Lamback, Alexandro Guterres, Monica R. Gadelha
Summary: This study identified miRNAs associated with resistance to fg-SRLs treatment in acromegaly using miRNA sequencing and bioinformatic analysis. It was found that miR-181a-5p, miR-181b-5p, and miR-383-5p were differentially expressed, with miR-181a-5p and miR-181b-5p downregulated and miR-383-5p upregulated in the uncontrolled group. ROC curve analysis showed that miR-383-5p had an NPV of 84.3% and a PPV of 84.5%. Thus, these miRNAs can serve as biomarkers and tools to guide clinical decisions in response to fg-SRLs treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Victor Passos Gibson, Houda Tahiri, Quoc Thang Phan, Pierre Hardy
Summary: Glioblastoma multiforme (GBM) is a common and lethal brain cancer, and current pharmacological interventions have limited effects. MicroRNA-181a-5p (miR-181a) is downregulated in GBM clinical samples and overexpression of miR-181a can negatively affect tumor growth. Researchers have developed an optimized nanocarrier, HA-LNP, which can efficiently deliver miR-181a to GBM cells and has therapeutic effects in vitro and in vivo.
Article
Oncology
Jiancheng Lv, Zijian Zhou, Jingzi Wang, Xiao Yang, Hao Yu, Jie Han, Dexiang Feng, Baorui Yuan, Qikai Wu, Pengchao Li, Qiang Lu, Haiwei Yang
Summary: The study found that circFAM114A2 is downregulated in urothelial carcinoma, with higher expression associated with better survival in patients and histological grade. This circRNA inhibits cell proliferation and enhances cisplatin sensitivity by sponging miR-222-3p/miR-146a-5p to regulate P27/P21 expression. CircFAM114A2 shows potential as a prognostic biomarker and therapeutic target for urothelial carcinoma.
FRONTIERS IN ONCOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Li Zuo, Xiaoli Li, Hailong Zhu, Anqi Li, Yonggang Wang
Summary: Detection of miR-181a in circulating tumor cells (CTCs) of ovarian cancer patients may allow for early diagnosis and prognostic evaluation. MiR-181a could be involved in the development and progression of ovarian cancer as an oncogene. The expression of miR-181a in CTCs captured by EpCAM liposome magnetic beads (Ep-LMBs) correlates with clinical characteristics of ovarian cancer.
Article
Medicine, Research & Experimental
Ajay K. Yadav, Teja Naveen Sata, Daksh Verma, Amit K. Mishra, Amrendra K. Sah, Md Musa Hossain, Kishor Pant, Senthil K. Venugopal
Summary: This study demonstrates that free fatty acids induce apoptosis in hepatic cells by upregulating miR-181a-5p expression, which may have implications for the development of non-alcoholic fatty liver disease.
Article
Medicine, Research & Experimental
Ju-Yeon Kim, Eun Jung Jung, Jae-Myung Kim, Youngsim Son, Han Shine Lee, Seung-Jin Kwag, Ji-Ho Park, Jin-Kyu Cho, Han-Gil Kim, Taejin Park, Sang-Ho Jeong, Chi-Young Jeong, Young-Tae Ju
Summary: miR-221 and miR-222 play a significant role in the progression and invasion of breast cancer cells by regulating the target gene ANXA3. The expression levels of miR-221/222 are associated with aggressive characteristics of breast cancer, and downregulation of ANXA3 through increased expression of miR-221/222 reduces breast cancer progression and increases the effectiveness of chemotherapy.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2023)
Article
Medicine, Research & Experimental
Aiqing Zhao, Wanlin Liu, Xiaolong Cui, Na Wang, Yuxin Wang, Liang Sun, Huiqin Xue, Lishuan Wu, Shuxia Cui, Yun Yang, Rui Bai
Summary: TUSC7 is downregulated in osteosarcoma tissues and cell lines, and its overexpression inhibits cell proliferation, migration, and invasion while promoting apoptosis. TUSC7 exerts its role in OS cells by sponging miR-181a, thereby mediating its tumor suppressor function through the miR-181a/RASSF6 axis, potentially representing a new therapeutic target for osteosarcoma.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Mahdiyar Iravani Saadi, Javad Moayedi, Fakhroddin Hosseini, Hossain Ali Rostamipour, Zahed Karimi, Zahra Rahimian, Maryam Ahmadyan, Zahra Ghahramani, Mehdi Dehghani, Karim Yousefi, Nadiya Kheradmand, Mani Ramzi, Nastaran Fooladivanda
Summary: This study investigated the therapeutic effects of natural anticancer agents resveratrol, gallic acid, and piperine on AML cell line and their influence on miRs expression. The results suggest that LNA-anti-miRs may have potential as a treatment for AML, and all three compounds showed promising anticancer effects in AML patients.
BIOCHEMICAL GENETICS
(2023)
Review
Geriatrics & Gerontology
Chulwoo Kim, Zhongde Ye, Cornelia M. Weyand, Jorg J. Goronzy
Summary: miR-181a plays a critical role in T cell development, activation, and antiviral responses, with its decreased expression in older individuals contributing to age-related immune defects. This review discusses the pathways regulated by miR-181a and potential strategies for restoring its expression to improve impaired T cell responses in the elderly.
Article
Endocrinology & Metabolism
Shaojian Lin, Yue Zhu, Chengcheng Ji, Weiming Yu, Cheng Zhang, Langping Tan, Miaoyun Long, Dingyuan Luo, Xinzhi Peng
Summary: This study suggests that the overexpression of METTL3 is associated with malignant behaviors in thyroid carcinoma (TC), and it promotes tumor development and metastasis by regulating the expression levels of miR-222-3p and STK4.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Nutrition & Dietetics
Johanna Valerio, Ana Barabash, Nuria Garcia de la Torre, Paz De Miguel, Veronica Melero, Laura Del Valle, Inmaculada Moraga, Cristina Familiar, Alejandra Duran, Maria Jose Torrejon, Angel Diaz, Ines Jimenez, Pilar Matia, Miguel Angel Rubio, Alfonso Luis Calle-Pascual
Summary: The San Carlos Gestational Diabetes Mellitus prevention study found that pregnant women with GDM had higher levels of leptin and insulin and lower levels of adiponectin. Additionally, a MedDiet-based nutritional intervention can reduce insulin, HOMA-IR, and Il-6 levels, and affect the expression of miR-222-3p and miR-103a-3p.
Article
Biochemistry & Molecular Biology
Pelin Telkoparan-Akillilar, Dilek Cevik
Summary: The study aimed to investigate the expression levels of miR-17, miR-21, miR-27a, miR-106b, miR-222, and CHOP in patients with atherosclerosis compared to healthy controls. It was found that miR-17 and miR-106b were significantly increased in patients, while miR-21, miR-27a, and miR-222 were significantly decreased. Additionally, CHOP gene was significantly induced in patient samples.
MOLECULAR BIOLOGY REPORTS
(2021)
Editorial Material
Cell & Tissue Engineering
Eirini P. Papapetrou
Summary: In this study, loss of LMNB1 gene was identified as a novel genetic basis for abnormal nuclear shape of neutrophils and alterations in hematopoietic stem and progenitor cell fate, which plays a role in promoting malignancy.
Article
Cell Biology
Svitlana Melnik, Nina Hofmann, Jessica Gabler, Nicole Hecht, Wiltrud Richter
Summary: The study identified miR-181a as a key regulator in chondrogenesis of MSC by targeting the RSPO2 mRNA, activating BMP signaling, and inhibiting WNT signaling, leading to hypertrophic differentiation. The disrupted correlation between miR-181a and miR-218 expression levels in osteoarthritis highlights the importance of WNT-BMP signaling crosstalk for preventing OA.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Orthopedics
Francisco Guerra-Pinto, Alirio Fabian, Tiago Mota, Tania Diaz, Mariano Monzo, Xavier Martin Oliva
Summary: This study aimed to investigate the anatomy of the multiple bundles of the deltoid ankle ligament, with a focus on the tibiocalcaneal ligament. The results showed that the tibiocalcaneal ligament was present in most specimens, while some variations were found in the connection between the tibia and the sustentaculum tali.
FOOT AND ANKLE SURGERY
(2021)
Article
Genetics & Heredity
Jose Marcos Moreno-Cabrera, Jesus del Valle, Lidia Feliubadalo, Marta Pineda, Sara Gonzalez, Olga Campos, Raquel Cuesta, Joan Brunet, Eduard Serra, Gabriel Capella, Bernat Gel, Conxi Lazaro
Summary: This study evaluated a next-generation sequencing-based CNV detection tool (DECoN) as a first-tier screening for genetic diagnostics in hereditary cancer. The results showed that DECoN could detect more CNVs while reducing turnaround time and costs.
JOURNAL OF MEDICAL GENETICS
(2022)
Editorial Material
Oncology
Jian-Guo Zhou, Zhaohui Huang, Ondrej Slaby, Alfons Navarro
FRONTIERS IN ONCOLOGY
(2021)
Editorial Material
Oncology
Alfons Navarro
Article
Oncology
Yanting Dong, Tong Qiu, Yunpeng Xuan, Ao Liu, Xiao Sun, Zhangfeng Huang, Wenhao Su, Wenxing Du, Tianxiang Yun, Yang Wo, Alfons Navarro, Wenjie Jiao
Summary: In this study, circFBXW7 was found to inhibit proliferation and migration of LUAD cell lines by controlling the miR-942-5p/BARX2 axis, with its levels correlating with patient survival. This suggests that regulating circFBXW7 could have therapeutic value in treating LUAD patients.
TRANSLATIONAL LUNG CANCER RESEARCH
(2021)
Article
Oncology
Jara Martin, Joan Josep Castellano, Ramon Maria Marrades, Jordi Canals, Nuria Vinolas, Tania Diaz, Laureano Molins, Daniel Martinez, Bing Han, Jorge Moises, Yangyi He, Mariano Monzo, Alfons Navarro
Summary: Circ-10720 regulates EMT, apoptosis, and proliferation in non-small cell lung cancer and acts as a biomarker for relapse.
TRANSLATIONAL LUNG CANCER RESEARCH
(2021)
Article
Health Care Sciences & Services
Jose Carlos Benitez, Marc Campayo, Tania Diaz, Carme Ferrer, Melissa Acosta-Plasencia, Mariano Monzo, Luis Cirera, Benjamin Besse, Alfons Navarro
Summary: The study demonstrated that lincRNA-p21 expression levels are associated with response to neoadjuvant CRT in rectal cancer patients, serving as a predictive biomarker. It is also correlated with tumor stage, tumor regression grade, and other indicators.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Oncology
Jordi Canals, Alfons Navarro, Cristina Vila, Josep M. Canals, Tania Diaz, Melissa Acosta-Plasencia, Corali Cros-Font, Bing Han, Yangyi He, Mariano Monzo
Summary: This study is the first to report that stimuli from the embryonic lung can modulate the malignant phenotype of lung cancer cells, control their growth capacity, and activate their differentiation into myofibroblasts. These findings could lead to new strategies for lung cancer management.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Cell Biology
Helena Mazuelas, Miriam Magallon-Lorenz, Juana Fernandez-Rodriguez, Itziar Uriarte-Arrazola, Yvonne Richaud-Patin, Ernest Terribas, Alberto Villanueva, Elisabeth Castellanos, Ignacio Blanco, Angel Raya, Jakub Chojnacki, Holger Heyn, Cleofe Romagosa, Conxi Lazaro, Bernat Gel, Meritxell Carrio, Eduard Serra
Summary: In this study, researchers developed an iPSC-based in vitro differentiation system for studying the development of plexiform neurofibromas (pNFs). They found that using a heterotypic spheroid model could generate tumors similar to human pNFs. Additionally, analysis of human pNF single-cell RNA sequences revealed the presence of subpopulations of Schwann cells at different differentiation states.
Article
Oncology
Leyre Bento, Oliver Vogler, Adriana Sas-Barbeito, Josep Muncunill, Teresa Ros, Jordi Martinez, Adriana Quintero-Duarte, Rafael Ramos, Victor Jose Asensio, Concepcion Fernandez-Rodriguez, Antonio Salar, Alfons Navarro, Raquel del Campo, Javier Ibarra, Regina Alemany, Antonio Gutierrez
Summary: This study identified three promising prognostic markers for diffuse large B cell lymphoma (DLBCL) patients at high risk of failure with standard therapy using microarray technology. The importance of these biomarkers in treatment response and survival was validated in both cell lines and patients. This study provides the possibility of early detection for DLBCL patients at high risk of treatment failure.
Article
Pathology
Gemma Bullich, Leslie Matalonga, Montserrat Pujadas, Anastasios Papakonstantinou, Davide Piscia, Rafael Artuch, Pia Gallano, Gloria Garrabou, Juan R. Gonzalez, Daniel Grinberg, Miriam Guitart, Steven Laurie, Conxi Lazaro, Cristina Luengo, Ramon Marti, Montserrat Mila, David Ovelleiro, Genis Parra, Aurora Pujol, Eduardo Tizzano, Alfons Macaya, Francesc Palau, Antonia Ribes, Luis A. Perez-Jurado, Sergi Beltran
Summary: Many rare disease patients remain undiagnosed even after genomic testing. Reanalysis of existing genomic data has shown to increase diagnostic yield. The Undiagnosed Rare Disease Program of Catalonia project reanalyzed genomic data from 323 families with a neurologic rare disease, leading to a diagnosis for 20.7% of the patients.
JOURNAL OF MOLECULAR DIAGNOSTICS
(2022)
Article
Biochemistry & Molecular Biology
Bing Han, Laureano Molins, Yangyi He, Nuria Vinolas, David Sanchez-Lorente, Marc Boada, Angela Guirao, Tania Diaz, Daniel Martinez, Jose Ramirez, Jorge Moises, Melissa Acosta-Plasencia, Mariano Monzo, Ramon M. Marrades, Alfons Navarro
Summary: By analyzing the cargo of extracellular vesicles (EVs) from the tumor-draining vein of the lung, researchers have identified a promising new biomarker (EV-miR-203a-3p) for predicting post-surgical relapse in patients with non-small cell lung cancer (NSCLC).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Miriam Magallon-Lorenz, Ernest Terribas, Sara Ortega-Bertran, Edgar Creus-Bachiller, Marco Fernandez, Gerard Requena, Inma Rosas, Helena Mazuelas, Itziar Uriarte-Arrazola, Alex Negro, Tereza Lausova, Elisabeth Castellanos, Ignacio Blanco, George DeVries, Hiroyuki Kawashima, Eric Legius, Hilde Brems, Viktor Mautner, Lan Kluwe, Nancy Ratner, Margaret Wallace, Juana Fernandez-Rodriguez, Conxi Lazaro, Jonathan A. Fletcher, David Reuss, Meritxell Carrio, Bernat Gel, Eduard Serra
Summary: This article presents the genomic characterization of 9 widely used human MPNST cell lines, providing valuable resources for translational research. NF1-related cell lines exhibited characteristics similar to primary MPNSTs, while sporadic cell lines showed different inactivation patterns of tumor suppressor genes and mutations. The re-classification of cell lines as melanomas and other sarcomas demonstrated different responses to drug treatment. Deep genomic analysis, methylome-based classification, and cell-identity marker expression challenged the identity of common MPNST cell lines, highlighting the need for revisions in MPNST diagnosis.
Article
Biochemical Research Methods
Helena Mazuelas, Itziar Uriarte-Arrazola, Juana Fernandez-Rodriguez, Miriam Magallon-Lorenz, Alberto Villanueva, Conxi Lazaro, Bernat Gel, Eduard Serra, Meritxell Carrio
Summary: This study generated neurofibromaspheres by differentiating NF1(-/-) Schwann cells from induced pluripotent stem cells and combining them with neurofibroma primary fibroblasts. Neurofibroma-like tumors were developed when these neurofibromaspheres were engrafted in the sciatic nerve of nude mice. This model provides a versatile platform for drug screening and the study of neurofibroma biology.
