期刊
LEUKEMIA
卷 27, 期 3, 页码 619-628出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/leu.2012.258
关键词
C/EBP beta; BCR-ABL; chronic myeloid leukemia
资金
- Japan Society for the Promotion of Science
- Global COE Program 'Center for Frontier Medicine' from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan
- Takeda Science Foundation
- Kobayashi Foundation for Cancer Research
- Senshin Medical Research Foundation
- Grants-in-Aid for Scientific Research [23591404, 25461415, 25112706, 25430149, 24390244, 24659461, 23890090] Funding Source: KAKEN
The BCR-ABL fusion oncoprotein accelerates differentiation and proliferation of myeloid cells during the chronic phase of chronic myeloid leukemia (CP-CML). Here, the role of CCAAT/enhancer binding protein beta (C/EBP beta), a regulator for 'emergency granulopoiesis,' in the pathogenesis of CP-CML was examined. C/EBP beta expression was upregulated in Lineage(-) CD34(+) CD38(-) hematopoietic stem cells (HSCs) and myeloid progenitors isolated from bone marrow of patients with CP-CML. In EML cells, a mouse HSC line, BCR-ABL upregulated C/EBP beta, at least in part, through the activation of STAT5. Myeloid differentiation and proliferation induced by BCR-ABL was significantly impaired in C/EBP beta-deficient bone marrow cells in vitro. Mice that were transplanted with BCR-ABL-transduced C/EBP beta knockout bone marrow cells survived longer than mice that received BCR-ABL-transduced wild-type (WT) bone marrow cells. Significantly higher levels of leukemic stem cells were maintained in BCR-ABL-transduced C/EBP beta-deficient cells than in BCR-ABL-transduced WT cells. These results suggest that C/EBP beta is involved in BCR-ABL-mediated myeloid expansion. Further elucidation of the molecular mechanisms underlying the C/EBP beta-mediated stem cell loss might reveal a novel therapeutic strategy for eradication of CML stem cells. Leukemia (2013) 27, 619-628; doi:10.1038/leu.2012.258
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