期刊
LEUKEMIA
卷 24, 期 1, 页码 162-168出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/leu.2009.206
关键词
SCID-repopulating cell; IBMI; CD34; CD38; hematopoietic stem cell; cord blood
资金
- Ministry of Education, Science and Culture of Japan [19591144, 21591251]
- Haiteku Research Center of the Ministry of Education
- Ministry of Education
- Promotion and Mutual Aid Corporation for Private Schools of Japan
- Kansai Medical University
- Japan Leukemia Research Foundation
- Mitsubishi Pharma Research Foundation
- Takeda Science Foundation
The identification of human CD34-negative (CD34(-)) hematopoietic stem cells (HSCs) provides a new concept for the hierarchy in the human HSC compartment. This study investigated the long-term repopulating capacity and redistribution kinetics of human cord blood-derived CD34(-) severe combined immunodeficiency (SCID)-repopulating cells (SRCs) and compared them with those of CD34(+)CD38(+) and CD34(+)CD38(-) SRCs using the intra-bone marrow injection (IBMI) to clarify the characteristics of CD34(-) SRCs. On the basis of the limiting dilution analyses data, estimated numbers of CD34(+)CD38(+), CD34(+)CD38(-), and CD34(-) SRCs were transplanted to NOD/SCID mice by IBMI. The human cell repopulation at the site of injection and the other bones were serially investigated. Interestingly, CD34(+)CD38(+), CD34(+)CD38(-), and CD34(-) SRCs began to migrate to other bones 2 and 5 weeks after the transplantation, respectively. Accordingly, the initiation of migration seemed to differ between the CD34(+) and CD34(-) SRCs. In addition, CD34(+)CD38(+) SRCs only sustained a short-term repopulation. However, both CD34(+)CD38(-) and CD34(-) SRCs had longer-term repopulation capacity. Taken together, these findings showed that CD34(-) SRCs show different in vivo kinetics, thus suggesting that the identified CD34(-) SRCs are a distinct class of primitive HSCs in comparison to the CD34(+)CD38(-) and CD34(+)CD38(-) SRCs. Leukemia (2010) 24, 162-168; doi:10.1038/leu.2009.206; published online 1 October 2009
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