Article
Hematology
Sabine Kayser, Robert K. Hills, Ralitsa Langova, Michael Kramer, Francesca Guijarro, Zuzana Sustkova, Elihu H. Estey, Carole M. Shaw, Zdenek Racil, Jiri Mayer, Pavel Zak, Maria R. Baer, Andrew M. Brunner, Tomas Szotkowski, Petr Cetkovsky, David Grimwade, Roland B. Walter, Alan K. Burnett, Anthony D. Ho, Gerhard Ehninger, Carsten Mueller-Tidow, Uwe Platzbecker, Christian Thiede, Christoph Roellig, Angela Schulz, Gregor Warsow, Benedikt Brors, Jordi Esteve, Nigel H. Russell, Richard F. Schlenk, Mark J. Levis
Summary: Patients with t(8;16) AML have poor outcomes with chemotherapy, but may see substantial improvement with allogeneic hematopoietic cell transplantation performed in first complete remission (CR1).
BRITISH JOURNAL OF HAEMATOLOGY
(2021)
Article
Pathology
Barina Aqil, Juehua Gao, Melissa Stalling, Madina Sukhanova, Eric J. Duncavage, Xinyan Lu, Kristy L. Wolniak, Friederike Kreisel, Nabeel R. Yaseen
Summary: This study identified 5 cases of AML with t(8;16) and highlighted their distinctive immunophenotypic profile and genomic features which present a diagnostic challenge.
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
(2022)
Article
Oncology
Yan Yu, Zhao Zeng, Jundan Xie, Qiongyu Lu, Wenzhi Cai, Ruixi Zhang, Jinlan Pan, Yun Zhao, Aining Sun, Huiying Qiu, Suning Chen
Summary: The PAX5-UBE2D4 fusion gene identified in a case of Ph-positive mixed phenotype acute leukemia has partial oncogenic activity, promoting tumor growth and cell proliferation.
FRONTIERS IN ONCOLOGY
(2021)
Article
Medicine, General & Internal
Malgorzata Galecka, Katarzyna Blizniewska-Kowalska, Piotr Galecki, Janusz Szemraj, Agata Orzechowska
Summary: This study aimed to evaluate the expression of the S100A10 gene at the mRNA and protein levels in patients with depressive disorders and to determine the impact of p11 in the etiopathogenesis of depression. The results indicate slightly increased S100A10 gene expression in patients with depression, but these values do not reach statistical significance.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Pathology
Arash Ronaghy, Shimin Hu, Zhenya Tang, Wei Wang, Guilin Tang, Sanam Loghavi, Shaoying Li, Beenu Thakral, L. Jeffrey Medeiros, Tariq Muzzafar
Summary: The rare cytogenetic abnormality t(3;12)(q26.2;p13) is associated with myeloid neoplasms, myelodysplasia, and poor prognosis. Common features include myelodysplasia, chromosome 7 abnormalities, and high blast counts. The presence of this abnormality is closely related to MDS-related changes, suggesting it should be included in the list of AML defining abnormalities in the WHO classification.
Article
Biochemistry & Molecular Biology
Tommaso Colangelo, Patrizio Panelli, Francesco Mazzarelli, Francesco Tamiro, Valentina Melocchi, Elisabetta De Santis, Roberto Cuttano, Orazio Palumbo, Giovanni Rossi, Fabrizio Bianchi, Vincenzo Giambra
Summary: T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive T-cell malignancy. Recent studies have shown that T-ALL-secreted small extracellular vesicles (sEV) contain NOTCH1-dependent microRNAs (EV-miRs), which regulate oncogenic pathways and promote the expansion and survival of highly proliferative cell subsets, thereby altering the cell heterogeneity and dynamics of leukemia.
Article
Hematology
Genki Yamato, Tomoko Kawai, Norio Shiba, Junji Ikeda, Yusuke Hara, Kentaro Ohki, Shin-Ichi Tsujimoto, Taeko Kaburagi, Kenichi Yoshida, Yuichi Shiraishi, Satoru Miyano, Nobutaka Kiyokawa, Daisuke Tomizawa, Akira Shimada, Manabu Sotomatsu, Hirokazu Arakawa, Souichi Adachi, Takashi Taga, Keizo Horibe, Seishi Ogawa, Kenichiro Hata, Yasuhide Hayashi
Summary: The study found that DNA methylation patterns were associated with genetic alterations in pediatric patients with acute myeloid leukemia (AML). Specific CpG sites were useful in categorizing patients into different clusters based on methylation levels, which were related to gene expression patterns and prognosis.
Article
Immunology
Wenpu Lai, Xiaofang Wang, Lian Liu, Ling Xu, Lipeng Mao, Jiaxiong Tan, Xianfeng Zha, Huien Zhan, Wen Lei, Yu Lan, Guobing Chen, Yangqiu Li, Oscar Junhong Luo
Summary: This study identified a subset of circulating CD103+ T cells in B-ALL patients, which showed different developmental potential into effector T cells or exhausted T cells, closely associated with patient prognosis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Hematology
Leonardo Luis Artico, Angelo Brunelli Albertoni Laranjeira, Livia Weijenborg Campos, Juliana Ronchi Correa, Priscila Pini Zenatti, Jose Barreto Campello Carvalheira, Sandra Regina Brambilla, Alexandre Eduardo Nowill, Silvia Regina Brandalise, Jose Andres Yunes
Summary: IGFBP7 plays an oncogenic role in acute lymphoblastic leukemia by promoting the perdurance of IGF1R at the cell surface, prolonging insulin/IGF stimulation. Clinical data suggest that IGFBP7 is a valid target for antibody-based therapeutic interventions in ALL.
Review
Oncology
Francesco Tamiro, Andrew P. Weng, Vincenzo Giambra
Summary: Leukemia-initiating cells (LIC) are unique cells in different types of leukemia that have self-renewing capabilities and produce tumors, which are functionally distinct from bulk leukemia cells. Current conventional treatments are not effective in eliminating LICs, hence innovative therapeutics targeting LICs hold promise for developing an effective cure for ALL.
Review
Pathology
Amy S. Duffield, Charles G. Mullighan, Michael J. Borowitz
Summary: The updated International Consensus Classification (ICC) of B-acute lymphoblastic leukemia (B-ALL) and T-acute lymphoblastic leukemia (T-ALL) includes revisions to previous subtypes and new entities. The classification incorporates recent clinical, cytogenetic, and molecular data, emphasizing whole transcriptome analysis and gene expression clustering studies. The new classification allows for improved risk stratification and optimized treatment plans for ALL patients.
Article
Biology
Zhiqiang Wang, Chunxiao Zhang, Charles David Warden, Zheng Liu, Yate-Ching Yuan, Chao Guo, Charles Wang, Jinhui Wang, Xiwei Wu, Richard Ermel, Steven L. Vonderfecht, Xiuli Wang, Christine Brown, Stephen Forman, Yaling Yang, M. James You, WenYong Chen
Summary: SIRT1 plays an unexpected role in promoting aging of HSCs and age-dependent MPAL. Knockout of Sirt1 improves the function of aging HSCs and suppresses the development of MPAL.
COMMUNICATIONS BIOLOGY
(2022)
Article
Oncology
Musa Yilmaz, Hagop M. Kantarjian, Gokce Toruner, C. Cameron Yin, Rashmi Kanagal-Shamanna, Jorge E. Cortes, Ghayyas Issa, Nicholas J. Short, Joseph D. Khoury, Guillermo Garcia-Manero, Farhad Ravandi, Tapan Kadia, Marina Konopleva, William G. Wierda, Nitin Jain, Zeev Estrov, Koji Sasaki, Sherry Pierce, Susan M. O'Brien, Elias J. Jabbour
Summary: Adult patients with t(1;19)(q23;p13) positive ALL have a favorable prognosis with intensive chemotherapy regimens.
LEUKEMIA & LYMPHOMA
(2021)
Article
Oncology
Gabriela D. A. Guardia, Rafaella G. Naressi, Vanessa C. Buzzato, Juliana B. da Costa, Ilana Zalcberg, Jordana Ramires, Bettina Malnic, Luciana M. Gutiyama, Pedro A. F. Galante
Summary: Acute myeloid leukemia (AML) is the most common type of acute leukemia in adults. A group of genes called olfactory receptors (ORs) were found to be highly expressed in AML cells. The expression of these ORs could predict the prognosis of AML patients and they have the potential to be used as biomarkers for diagnosis and as targets for new drugs to treat AML.
Article
Biochemistry & Molecular Biology
Mina Noura, Hidemasa Matsuo, Takahiko Yasuda, Shinobu Tsuzuki, Hitoshi Kiyoi, Fumihiko Hayakawa
Summary: TAL1 is frequently dysregulated in T-ALL, and its overexpression is associated with unfavorable clinical outcomes. This study found that the tumor suppressor KLF4 can suppress SE-driven TAL1 expression in T-ALL cells by inhibiting the formation of 5' TAL1 super-enhancer. A small molecule inducer of KLF4, APTO-253, exerts an anti-leukemic effect by targeting SE-driven TAL1 expression in T-ALL cells. Induction of KLF4 may be a promising strategy to control TAL1 expression and could serve as a novel treatment for T-ALL patients with poor prognosis.
Article
Medical Laboratory Technology
Vincent H. J. van der Velden, Frank Preijers, Ulrika Johansson, Theresia M. Westers, Alan Dunlop, Anna Porwit, Marie C. Bene, Peter Valent, Jeroen te Marvelde, Orianne Wagner-Ballon, Uta Oelschlaegel, Leonie Saft, Sharham Kordasti, Robin Ireland, Eline Cremers, Canan Alhan, Carolien Duetz, Willemijn Hobo, Nicolas Chapuis, Michaela Fontenay, Peter Bettelheim, Lisa Eidenshink-Brodersen, Patricia Font, Michael R. Loken, Sergio Matarraz, Kiyoyuki Ogata, Alberto Orfao, Katherina Psarra, Dolores Subira, Denise A. Wells, Matteo G. Della Porta, Kate Burbury, Frauke Bellos, Elisabeth Weiss, Wolfgang Kern, Arjan van de Loosdrecht
Summary: This article reviews the progress of flow cytometry (FCM) in the diagnosis of myelodysplastic syndrome (MDS) in recent years, and provides optimal methods and recommendations for sample processing, antibody panels and fluorochromes, and current hardware technologies. These recommendations will facilitate the appropriate application of FCM assays in the diagnostic workup of MDS patients, but further standardization and harmonization are needed.
CYTOMETRY PART B-CLINICAL CYTOMETRY
(2023)
Article
Medical Laboratory Technology
Elisabeth Weiss, Wencke Walter, Manja Meggendorfer, Constance Baer, Claudia Haferlach, Torsten Haferlach, Wolfgang Kern
Summary: Myelodysplastic syndromes (MDS) is a group of diseases, and we have identified a specific immunophenotype and genetic mutations in some MDS cases, with a consistent gene expression profile. These findings may contribute to defining a new sub-entity of MDS.
CYTOMETRY PART B-CLINICAL CYTOMETRY
(2023)
Article
Medical Laboratory Technology
Orianne Wagner-Ballon, Peter Bettelheim, Jeroen Lauf, Frauke Bellos, Matteo Della Porta, Erica Travaglino, Dolores Subira, Irene Nuevo Lopez, Sihem Tarfi, Theresia M. Westers, Ulrika Johansson, Katherina Psarra, Serafeim Karathanos, Sergio Matarraz, Enrique Colado, Monali Gupta, Robin Ireland, Wolfgang Kern, Arjan A. Van de Loosdrecht
Summary: This multicenter study demonstrates the robustness of the monocyte assay in distinguishing chronic myelomonocytic leukemia from other causes of monocytosis. It also confirms the possibility of using this assay in bone marrow samples.
CYTOMETRY PART B-CLINICAL CYTOMETRY
(2023)
Article
Medical Laboratory Technology
Arjan A. Loosdrecht, Wolfgang Kern, Anna Porwit, Peter Valent, Sharham Kordasti, Eline Cremers, Canan Alhan, Carolien Duetz, Alan Dunlop, Willemijn Hobo, Frank Preijers, Orianne Wagner-Ballon, Nicolas Chapuis, Michaela Fontenay, Peter Bettelheim, Lisa Eidenschink-Brodersen, Patricia Font, Ulrika Johansson, Michael R. Loken, Jeroen G. Marvelde, Sergio Matarraz, Kiyoyuki Ogata, Uta Oelschlaegel, Alberto Orfao, Katherina Psarra, Dolores Subira, Denise A. Wells, Marie C. Bene, Matteo G. Della Porta, Kate Burbury, Frauke Bellos, Vincent H. J. van der Velden, Theresia M. Westers, Leonie Saft, Robin Ireland
Summary: This article discusses the rationale for including flow cytometry (FCM) in the diagnostic investigation and evaluation of cytopenias and suspected myelodysplastic syndromes (MDS) by the European LeukemiaNet international MDS Flow Working Group. The WHO 2016 classification acknowledges the contribution of FCM in the diagnosis of MDS and its potential usefulness in prognostication, prediction, therapy evaluation, and patient follow-up.
CYTOMETRY PART B-CLINICAL CYTOMETRY
(2023)
Article
Geriatrics & Gerontology
Aron S. Buchman, Lei Yu, Hans-Ulrich Klein, Andrea R. Zammit, Shahram Oveisgharan, Francine Grodstein, Shinya Tasaki, Allan Levey, Nicholas T. Seyfried, David A. Bennett
Summary: This study identified cortical proteins that may provide motor resilience in older adults. Five proteins were associated with progressive parkinsonism, suggesting their potential as therapeutic targets for maintaining motor function despite untreatable brain pathologies.
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2023)
Article
Clinical Neurology
Andrea R. Zammit, Yu Lei, Vladislav Petyuk, Julie A. Schneider, Philip Lawrence De Jager, Hans-Ulrich Klein, David A. Bennett, Aron S. Buchman
Summary: This study examines whether aggregating cortical peptides associated with cognitive resilience into an index can identify older adults with higher or lower cognitive resilience. The results show that a higher index is associated with slower cognitive decline, slower motor decline, lower burden of AD pathologies, and modifies the negative effects of AD pathology on AD dementia proximate to death. These findings suggest that cortical proteins may be potential therapeutic targets for maintaining brain health in aging adults.
Editorial Material
Medical Laboratory Technology
Wolfgang Kern, Arjan van de Loosdrecht
CYTOMETRY PART B-CLINICAL CYTOMETRY
(2023)
Article
Medical Laboratory Technology
Wolfgang Kern, Theresia M. Westers, Frauke Bellos, Marie Christine Bene, Peter Bettelheim, Lisa Eidenschink Brodersen, Kate Burbury, Sung-Chao Chu, Matthew Cullen, Matteo Della Porta, Alan Stewart Dunlop, Ulrika Johansson, Sergio Matarraz, Uta Oelschlaegel, Kiyoyuki Ogata, Anna Porwit, Frank Preijers, Katherina Psarra, Leonie Saft, Dolores Subira, Elisabeth Weiss, Vincent H. J. van der Velden, Arjan van de Loosdrecht
Summary: This study evaluated the use of pre-defined flow cytometric markers in the diagnosis of MDS and CMML. The results showed that flow cytometry had a high concordance with the diagnosis of MDS, and could identify cases "in agreement with MDS". Furthermore, the study suggested that a 3% cut-off for myeloid progenitor cells in flow cytometry was more appropriate than the cytomorphologically defined cut-off of 5%.
CYTOMETRY PART B-CLINICAL CYTOMETRY
(2023)
Letter
Oncology
Constance Baer, Sandra Huber, Stephan Hutter, Manja Meggendorfer, Niroshan Nadarajah, Wencke Walter, Uwe Platzbecker, Katharina S. Goetze, Wolfgang Kern, Torsten Haferlach, Gregor Hoermann, Claudia Haferlach
Correction
Oncology
Janine Mueller, Wencke Walter, Claudia Haferlach, Heiko Mueller, Irene Fuhrmann, Martha-Lena Mueller, Henning Ruge, Manja Meggendorfer, Wolfgang Kern, Torsten Haferlach, Anna Stengel
Letter
Oncology
Janine Mueller, Wencke Walter, Claudia Haferlach, Heiko Mueller, Irene Fuhrmann, Martha-Lena Mueller, Henning Ruge, Manja Meggendorfer, Wolfgang Kern, Torsten Haferlach, Anna Stengel
Article
Oncology
Maki Sakuma, Piers Blombery, Manja Meggendorfer, Claudia Haferlach, Markus Lindauer, Uwe M. Martens, Wolfgang Kern, Torsten Haferlach, Wencke Walter
Summary: The study identified three somatic mutations in the UBA1 gene that are associated with the development of VEXAS syndrome. Additionally, the analysis revealed several putative somatic variants in UBA1 that may contribute to myeloid malignancies, particularly in patients with vacuoles or immunodysregulatory symptoms.
Letter
Oncology
Sandra Huber, Torsten Haferlach, Heiko Mueller, Manja Meggendorfer, Stephan Hutter, Gregor Hoermann, Constance Baer, Wolfgang Kern, Claudia Haferlach
Article
Oncology
Sandra Huber, Constance Baer, Stephan Hutter, Frank Dicker, Manja Meggendorfer, Christian Pohlkamp, Wolfgang Kern, Torsten Haferlach, Claudia Haferlach, Gregor Hoermann
Summary: According to the new classification standards, there have been changes in the diagnosis of leukemia patients, with an increase in the proportion of certain subtypes and the largest group still being genetically defined. The new classifications focus on genetics-based definitions with similar basic concepts and a large degree of agreement.
Letter
Oncology
Sandra Huber, Constance Baer, Stephan Hutter, Frank Dicker, Irene Fuhrmann, Manja Meggendorfer, Christian Pohlkamp, Wolfgang Kern, Torsten Haferlach, Claudia Haferlach, Gregor Hoermann
