期刊
LETTERS IN APPLIED MICROBIOLOGY
卷 59, 期 2, 页码 238-246出版社
WILEY-BLACKWELL
DOI: 10.1111/lam.12270
关键词
bacterial chromosome; ERIC PCR; gyrB; nodA; symbiotic plasmids
资金
- BBSRC
- Rothamsted International
- Biotechnology and Biological Sciences Research Council [BBS/E/C/00005196] Funding Source: researchfish
- BBSRC [BBS/E/C/00005196] Funding Source: UKRI
The nitrogen-fixing symbiosis between Rhizobium leguminosarum and host legumes is recognized as a key part of sustainable agriculture. A culture collection containing rhizobia isolated from legumes of economic importance in the UK and worldwide, maintained at Rothamsted Research for many years, provided material for this study. We aimed to develop and validate efficient molecular diagnostics to investigate whether the host plant or geographical location had a greater influence on the genetic diversity of rhizobial isolates, and the extent to which the core bacterial genome and the accessory symbiosis genes located on plasmids were affected. To achieve this, core housekeeping genes and those involved in symbiosis interactions were sequenced and compared with genome-sequenced strains in the public domain. Results showed that some Rh. leguminosarum symbiovar trifolii strains nodulating clovers and Rh. leguminosarum sv. viciae strains nodulating peas and vicias shared identical housekeeping genes, clover nodule isolates from the same location could have divergent symbiosis genes, and others isolated on different continents could be very similar. This illustrates the likely co-migration of rhizobia and their legume hosts when crops are planted in new areas and indicates that selective pressure may arise from both local conditions and crop host genotypes.
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