期刊
LETTERS IN APPLIED MICROBIOLOGY
卷 57, 期 4, 页码 259-265出版社
WILEY
DOI: 10.1111/lam.12113
关键词
Bacillus; hydrogen peroxide; hypochlorite; spores; spore germination; sterilization
资金
- U.S. Army Department of Defense Multidisciplinary University Research Initiative through the U.S. Army Research Laboratory
- U.S. Army Research Office [W911F-09-1-0286]
More than 95% of individuals in populations of Bacillus subtilis spores killed approximately 95% by hydrogen peroxide or hypochlorite germinated with a nutrient, although the germination of the treated spores was slower than that of untreated spores. The slow germination of individual oxidizing agent-treated spores was due to: (i) 3- to 5-fold longer lag times (T-lag) between germinant addition and initiation of fast release of spores' large dipicolinic acid (DPA) depot (ii) 2- to 10-fold longer times (T-release) for rapid DPA release, once this process had been initiated; and (iii) 3- to 7-fold longer times needed for lysis of spores' peptidoglycan cortex. These results indicate that effects of oxidizing agent treatment on subsequent spore germination are on: (i) nutrient germinant receptors in spores' inner membrane (ii) components of the DPA release process, possibly SpoVA proteins also in spores' inner membrane, or the cortex-lytic enzyme CwlJ; and (iii) the cortex-lytic enzyme SleB, also largely in spores' inner membrane. This study further indicates that rapid assays of spore viability based on measurement of DPA release in spore germination can give false-positive readings.
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