期刊
LETTERS IN APPLIED MICROBIOLOGY
卷 46, 期 5, 页码 526-535出版社
WILEY
DOI: 10.1111/j.1472-765X.2008.02357.x
关键词
alkaline active xylanases; ITS and 26S rDNA sequences; molecular characterization; RFLP of 18S rDNA; thermophilic fungi
Aims: Molecular characterization of commercially important group of xylanase producing thermophilic/thermotolerant fungi. Methods and Results: DNA from 16 thermophilic/thermotolerant fungal isolates was amplified by PCR using three sets of primers: (i) internal transcribed spacer sequence (ITSI-5.8S-ITSII), (ii) D1/D2 hyper variable region of 26S rDNA and (iii) 18S rDNA region. The amplified products of internal transcribed spacers (ITS) and D1/D2 region were sequenced and analysed using CLUSTALX, whereas, amplified 18S rDNA region was subjected to RFLP analysis based on restriction digestion with RsaI, MboI and Hinf I. Conclusions: The sequence based analyses of ITSI-5.8S-ITSII as compared with D1/D2 region of 26-28S rDNA was found to be a better tool for phylogenetic resolution of thermophilic/thermotolerant fungi. The ITSI-5.8S-ITSII sequence-based dendrogram indicates an early divergence of the alkaline active xylanase producing thermophilic fungal strains. Significance and Impact of the Study: This study was the first report on phylogenetic characterization of thermophilic/thermotolerant fungi.
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