Article
Multidisciplinary Sciences
Giorgio Tortarolo, Alessandro Zunino, Francesco Fersini, Marco Castello, Simonluca Piazza, Colin J. R. Sheppard, Paolo Bianchini, Alberto Diaspro, Sami Koho, Giuseppe Vicidomini
Summary: The feasibility of super-resolution microscopy for live and thick sample imaging is limited. We propose a method that combines ISM and STED techniques to achieve high-resolution imaging without introducing photodamage and background degradation.
NATURE COMMUNICATIONS
(2022)
Article
Optics
Pierre Jouchet, Clement Cabriel, Nicolas Bourg, Marion Bardou, Christian Pous, Emmanuel Fort, Sandrine Leveque-Fort
Summary: The modulated localization microscopy achieves high molecular localization precision through an illumination modulation method, improving axial localization precision while maintaining fast acquisition speed. Compared to existing super-resolution fluorescence microscopy, this method offers unique advantages.
Article
Biochemical Research Methods
Koen J. A. Martens, Abbas Jabermoradi, Suyeon Yang, Johannes Hohlbein
Summary: In single-molecule localization microscopy, integrating various PSF modalities into the phasor-based framework enables fast and accurate localization of single-molecule emitters in three dimensions. This approach is crucial for analyzing complex PSFs and achieving high precision in localization.
Article
Biochemical Research Methods
Mark Bates, Jan Keller-Findeisen, Adrian Przybylski, Andreas Hueper, Till Stephan, Peter Ilgen, Angel R. Cereceda Delgado, Elisa D'Este, Alexander Egner, Stefan Jakobs, Steffen J. Sahl, Stefan W. Hell
Summary: The dynamic model of the 4Pi point spread function allows for high three-dimensional resolution in localization microscopy. 4Pi-STORM imaging reveals nanoscale details of protein and nucleic acid organization in neurons and mitochondria. Despite challenges in instrumentation and data analysis, the development of a 4Pi-STORM microscope with dynamic PSF modeling and simpler optical design offers optimal resolution and accuracy for molecular organization studies.
Article
Biophysics
Rene Siegmund, Frank Werner, Stefan Jakobs, Claudia Geisler, Alexander Egner
Summary: Fluorescence microscopy and STED microscopy are important tools for studying cellular structures and biochemical processes, with STED microscopy offering high resolution in the range of a few tens of nanometers. The isoSTED microscope, utilizing water-immersion objective lenses, can achieve high-resolution imaging of cellular structures at room temperature, with a fast imaging speed.
BIOPHYSICAL JOURNAL
(2021)
Article
Optics
Dingpeng Liao, Fengliang Dong, Kun Zhang, Yi Zhou, Gaofeng Liang, Zhihai Zhang, Zhongquan Wen, Zhengguo Shang, Gang Chen, Luru Dai, Weiguo Chu
Summary: Superoscillation metalenses have the potential to break theoretical diffraction limitations in optical devices and systems. However, most current superoscillation metalenses have a limited field of view, which restricts their applications in imaging and microscopy. This study presents a high-NA superoscillation metalens with an entrance aperture stop that achieves superoscillation focusing within a larger field of view, offering a promising solution for superoscillation imaging and fast-scanning label-free farfield superoscillation microscopy.
PHOTONICS RESEARCH
(2022)
Article
Biochemical Research Methods
Lusheng Gu, Yuanyuan Li, Shuwen Zhang, Maoge Zhou, Yanhong Xue, Weixing Li, Tao Xu, Wei Ji
Summary: The ROSE-Z method achieves super-resolution imaging with a <2 nm axial localization precision using an asymmetric optical scheme and approximately 3,000 photons. This method demonstrates nanoscale three-dimensional and two-color imaging, enhancing the investigation of cellular nanostructures.
Review
Anatomy & Morphology
Samira Jadavi, Paolo Bianchini, Ornella Cavalleri, Silvia Dante, Claudio Canale, Alberto Diaspro
Summary: Atomic force microscopy (AFM) is a nano-mechanical tool used for biological studies at the molecular scale, measuring surface topography, mechanical, and electrical properties. Integrating AFM with super-resolved fluorescence microscopy addresses the resolution mismatch issue, while also utilizing the label-free imaging capabilities of AFM to complement fluorescence images. Recent research highlights the importance of combining these techniques in biological research for better specificity and resolution.
MICROSCOPY RESEARCH AND TECHNIQUE
(2021)
Article
Multidisciplinary Sciences
Sanghyeon Park, Yonghyeon Jo, Minsu Kang, Jin Hee Hong, Sangyoon Ko, Suhyun Kim, Sangjun Park, Hae Chul Park, Sang-Hee Shim, Wonshik Choi
Summary: The authors applied label-free wavefront sensing adaptive optics to single-molecule localization microscopy (SMLM) for deep-tissue super-resolution imaging. The proposed system allows for the measurement and correction of complex tissue aberrations, enabling SMLM to resolve sub-diffraction morphologies in deep tissues.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Xin Guo, Rui Pu, Zhimin Zhu, Shuqian Qiao, Yusen Liang, Bingru Huang, Haichun Liu, Lucia Labrador-Paez, Uliana Kostiv, Pu Zhao, Qiusheng Wu, Jerker Widengren, Qiuqiang Zhan
Summary: The authors introduce a new nanoscopy technique called stimulated-emission induced excitation depletion (STExD), which uses a single pair of low-power, near-infrared, continuous-wave lasers to inhibit the emission of multichromatic probes. By manipulating the common sensitizer in lanthanide upconversion systems, emission inhibition is achieved for a wide range of emitters, with high depletion efficiency and low saturation intensity.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Zhichao Du, Wenjing Wang, Siyuan Luo, Lingjie Zhang, Shuowei Yuan, Yongzhen Hei, Zhangbin Bao, Chunlai Chen, Yi Lin, Ling Chu
Summary: We have developed a self-renewable tag (srTAG) for protein fluorescence imaging. By leveraging the equilibrium between the fluorescent zwitterion and non-fluorescent spirocyclic form on the protein, as well as reversible fluorescence labeling, the srTAG enables self-recovery of fluorescence after photobleaching. Compared to other commonly used self-labeling tags, this small-sized srTAG allows 2-6 times longer imaging duration and is compatible with fluorophores with different spectral properties. This study provides a new strategy for fine-tuning self-labeling tags.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Multidisciplinary Sciences
Ankit Butola, David A. Coucheron, Karolina Szafranska, Azeem Ahmad, Hong Mao, Jean-Claude Tinguely, Peter McCourt, Paramasivam Senthilkumaran, Dalip Singh Mehta, Krishna Agarwal, Balpreet Singh Ahluwalia
Summary: This study presents an integrated chip-based optical nanoscopy combined with quantitative phase microscopy for obtaining 3D morphology of liver sinusoidal endothelial cells (LSEC). The multimodal microscope offers nanoscale visualization of both the lateral size and the thickness map of subcellular structures, providing estimates for fenestration diameter and sieve plate thickness. Treatment with cytochalasin B demonstrated the potential for precise detection of cell height, showcasing the versatility and precision of the proposed technique in bioimaging.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Optics
Brendan M. Heffernan, Peter S. Riley, Omkar D. Supekar, Stephanie A. Meyer, Diego Restrepo, Mark E. Siemens, Emily A. Gibson, Juliet T. Gopinath
Summary: This article presents a solution for imaging sub-diffraction dynamics of neural nanostructures involved in learning and memory using a two-photon, fiber-coupled, stimulated emission depletion microscope. The capabilities of this technique are demonstrated through super-resolution imaging of mammalian cells.
Article
Chemistry, Organic
Ramona Birke, Julia Ast, Dorien A. Roosen, Joon Lee, Kilian Rossmann, Christiane Huhn, Bettina Mathes, Michael Lisurek, David Bushiri, Han Sun, Ben Jones, Martin Lehmann, Joshua Levitz, Volker Haucke, David J. Hodson, Johannes Broichhagen
Summary: This study reports a method to make dyes impermeable for surface protein analysis. By synthesizing dyes with charged sulfonate groups and combining them with red and far-red dyes, Sulfo549 and Sulfo646 are obtained. These dyes are further linked to benzylguanine and choloralkane substrates for rapid cell fixation and protein labeling. The dyes have desirable photophysical properties and can differentiate proteins labeled in extracellular and intracellular compartments through co-labeling with permeable rhodamine. Additionally, these dyes can be used for transfection of neurons and imaging with super-resolution microscopy. This work provides a new approach for developing dyes with cellular impermeability and has important implications for accurate protein analysis with intracellular labeling.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2022)
Article
Physics, Applied
Oshri Rabinovich, Ariel Epstein
Summary: The study introduces a new method using metagratings to shape nonradiative field patterns in transmission, generating desired reactive near-field profiles with deep subwavelength features. This approach can directly produce fabrication-ready printed-circuit-board configurations, allowing versatile control of reactive near fields.
APPLIED PHYSICS LETTERS
(2021)
Review
Biochemical Research Methods
Colin J. R. Sheppard
JOURNAL OF BIOMEDICAL OPTICS
(2020)
Article
Optics
Rong Su, Jeremy Coupland, Colin Sheppard, Richard Leach
Summary: Surface topography measuring interference microscopy is a 3D imaging technique that quantitatively analyzes industrial and biomedical specimens. This paper presents a unified theoretical framework for 3D image formation in interference microscopy and discusses the linear relationship between scattered amplitude and surface topography. The study shows that the 3D filtering process can be reduced to two dimensions for near planar surfaces.
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION
(2021)
Article
Biochemical Research Methods
Aymeric Le Gratiet, Artemi Bendandi, Colin J. R. Sheppard, Alberto Diaspro
Summary: Many significant improvements in resolution in optical microscopy techniques are achieved by reducing scattering effects. Polarimetry-based imaging allows discrimination between scattering phenomena and experimental noise, providing additional structural information of a sample. The use of coherency matrix and related decompositions leads to improved imaging contrast without requiring complicated algebraic operations or prior knowledge of the sample.
JOURNAL OF BIOPHOTONICS
(2021)
Review
Multidisciplinary Sciences
Colin J. R. Sheppard
Summary: Structured illumination microscopy and image scanning microscopy are two rapidly growing microscopic techniques that can enhance transverse spatial resolution and/or improve axial imaging performance. This article reviews the history and principles of these techniques and compares the imaging properties of the two methods.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES
(2021)
Article
Optics
Colin J. R. Sheppard, Marco Castello, Giorgio Tortarolo, Eli Slenders, Takahiro Deguchi, Sami Koho, Paolo Bianchini, Giuseppe Vicidomini, Alberto Diaspro
Summary: In image scanning microscopy, a detector array is used instead of a pinhole in a confocal microscope, allowing for maximum likelihood restoration and compensation for aberrations. Illumination with a vortex doughnut beam is investigated as an example of aberration, leading to a more compact point spread function, higher resolution, and signal level compared to conventional microscopy.
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION
(2021)
Article
Optics
Colin J. R. Sheppard, Artemi Bendandi, Aymeric Le Gratiet, Alberto Diaspro
Summary: This article reexamines the measures of purity for 3D partially polarized fields, focusing on the separation of circularly and linearly polarized contributions and introducing a new degree of total linear polarization. Explicit expressions for the characteristic decomposition based on coherency matrix elements are presented, including the special case of an intrinsic coherency matrix. The parameterization of the coherency matrix in terms of ellipticity, and the directions of the ellipse normal and major axis are investigated. The discussion also includes phase consistency and provides a comprehensive collection of results regarding intrinsic polarization properties.
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION
(2022)
Review
Chemistry, Multidisciplinary
Colin J. R. Sheppard
Summary: Various optical methods of super-resolution microscopy, including confocal microscopy, structured illumination, nonlinear microscopy, and image scanning microscopy, are reviewed for their ability to observe delicate biological samples without strong invasive interaction. The discussion also covers the concepts of resolution and the basic principles of superresolution.
APPLIED SCIENCES-BASEL
(2021)
Article
Optics
Colin J. R. Sheppard, Artemi Bendandi, Aymeric Le Gratiet, Alberto Diaspro
Summary: Depolarization is an important contrast mechanism in biological and medical imaging. This paper presents a historical review on polarization algebra, measures of depolarization, and purity spaces, and discusses their connections with the eigenvalues of the coherency matrix. The advantages of a barycentric eigenvalue space are outlined, and a new parameter called diattenuation-corrected purity is introduced. The use of a combination of eigenvalues of coherency matrices to specify the depolarization condition is proposed. The relationships between optical and polarimetric radar formalisms are also reviewed.
Review
Optics
Colin J. R. Sheppard, Artemi Bendandi, Aymeric Le Gratiet, Alberto Diaspro
Summary: Many books on polarization provide tables of Mueller matrices. The coherency matrix is useful for interpreting Mueller matrices. We present a table of Mueller matrices M, coherency matrices C, and coherency matrix factors F for different polarization components and systems, along with simplified forms for certain cases.
Article
Optics
Colin J. R. Sheppard, Marco Castello, Giorgio Tortarolo, Alessandro Zunino, Eli Slenders, Paolo Bianchini, Giuseppe Vicidomini, Alberto Diaspro
Summary: This article reviews the properties of signal strength and integrated intensity in a scanned imaging system, with a particular focus on confocal imaging systems, including image scanning microscopy. The integrated intensity, which represents the image of a uniform planar object rather than the peak of the point spread function, serves as a measure of the flux in an image. Analytic expressions are provided for the intensity in the detector plane for a uniform volume object and its resulting background. The article also demonstrates the variation in integrated intensity with defocus for an offset point detector, which remains unchanged regardless of pixel reassignment. Furthermore, it reveals that the intensity in the detector plane carries defocus information, and simple processing of recorded data can enhance optical sectioning and background rejection.
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION
(2023)
Article
Multidisciplinary Sciences
Giorgio Tortarolo, Alessandro Zunino, Francesco Fersini, Marco Castello, Simonluca Piazza, Colin J. R. Sheppard, Paolo Bianchini, Alberto Diaspro, Sami Koho, Giuseppe Vicidomini
Summary: The feasibility of super-resolution microscopy for live and thick sample imaging is limited. We propose a method that combines ISM and STED techniques to achieve high-resolution imaging without introducing photodamage and background degradation.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Wei Zheng, Shan S. Kou, Colin J. R. Sheppard, Maitreyee Roy
Summary: Optical coherence microscopy (OCM) is a variant of OCT that uses a high-numerical aperture lens. Full-field OCM (FF-OCM) is an emerging non-invasive, label-free, interferometric technique for micron-scale imaging of surface structures or semi-transparent biomedical objects. In this study, a unique geometric phase-shifter based on ferroelectric liquid crystal technology is demonstrated to achieve achromatic phase-shifting for rapid three-dimensional imaging in a FF-OCM system, by imaging tissues and cells.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Optics
Colin J. R. Sheppard, Marco Castello, Giorgio Tortarolo, Alessandro Zunino, Eli Slenders, Paolo Bianchini, Giuseppe Vicidomini, Alberto Diaspro
Summary: We discuss the properties of signal strength and integrated intensity in two-photon excitation confocal microscopy and image scanning microscopy. The resolution, optical sectioning, and background rejection are all improved over nonconfocal two-photon microscopy. Replacing the pinhole of confocal two-photon microscopy with a detector array increases the peak intensity of the point spread function. The outer pixels of a detector array give signals from defocused regions, and thus the processing of these, such as through subtraction, can further improve optical sectioning and background rejection.
Article
Optics
Colin J. R. Sheppard, Marco Castello, Giorgio Tortarolo, Alessandro Zunino, Eli Slenders, Paolo Bianchini, Giuseppe Vicidomini, Alberto Diaspro
Summary: This article discusses the effects of image scanning microscopy using doughnut beam illumination on signal strength and integrated intensity properties. Doughnut beam illumination provides better optical sectioning and background rejection compared to Airy disk illumination. The outer pixels of a detector array capture signals from defocused regions, and digital processing of these signals can further enhance optical sectioning and background rejection in a single in-focus scan.
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION
(2023)
Proceedings Paper
Engineering, Electrical & Electronic
Giorgio Tortarolo, Simonluca Piazza, Andrea Bucci, Paolo Bianchini, Colin J. R. Sheppard, Alberto Diaspro, Eli Slenders, Sami Koho, Marco Castello, Giuseppe Vicidomini
2021 CONFERENCE ON LASERS AND ELECTRO-OPTICS EUROPE & EUROPEAN QUANTUM ELECTRONICS CONFERENCE (CLEO/EUROPE-EQEC)
(2021)
