Review
Oncology
Jordana Williams, Morris Kostiuk, Vincent L. Biron
Summary: HPV is responsible for most cervical cancers and some head and neck cancers. Specific detection methods are used to diagnose HPV infections in cervical and head and neck cancers.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Krisztina Nikovics, Cedric Castellarin, Xavier Holy, Marjorie Durand, Halima Morin, Abdelhafid Bendahmane, Anne-Laure Favier
Summary: Understanding bone homeostasis and regeneration is of great clinical and scientific importance for the development of bone therapy. Macrophages play a crucial role in bone regeneration during healing. In situ hybridization and hybridization chain reaction can be used to explore gene expression in bone tissues.
Review
Virology
Farideh Aghaeipour, Hamid Salehiniya, Hamid Abbaszadeh
Summary: The prevalence of HPV in oral lesions in Iran was found to be 21%, with the highest rates in Rasht (50%) and lip lesions (40%), and the lowest in normal mucosa (8%). The highest prevalence was observed in precancerous lesions (29%) and hairy leukoplakia (70%). Considering the fairly high prevalence of HPV, it is recommended to pay more attention to vaccination and treatment for HPV in Iran.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Genetics & Heredity
Emma Sicherre, Anne-Laure Favier, Diane Riccobono, Krisztina Nikovics
Summary: The study found that different antibodies for investigating CD206 expression may result in varied expression patterns during immunodetection, potentially leading to false results. Therefore, researchers need to carefully choose methods for characterizing macrophages. HCR is shown to be an excellent alternative method for detecting macrophages in situ.
Article
Infectious Diseases
Sheila B. Buijs, Jesper M. Weehuizen, Tim K. Jensen, Mette Boye, Mirjam H. A. Hermans, Peet T. G. A. Nooijen, Andy IM. Hoepelman, Chantal P. Bleeker-Rovers, Jan Jelrik Oosterheert, Peter C. Wever
Summary: This study assesses the value of fluorescence in situ hybridization (FISH) for detecting Coxiella burnetii in tissue samples from chronic Q fever patients. The results indicate that FISH has limited sensitivity and specificity, and its value in routine diagnostics for chronic Q fever is limited.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Microbiology
Leah J. Ricketson, Ravinder Lidder, Robyn Thorington, Irene Martin, Otto G. Vanderkooi, Manish Sadarangani, James D. Kellner
Summary: Research on nasopharyngeal carriage in healthy school-age children showed that despite the universal childhood PCV13 immunization, a significant proportion of samples still had detectable vaccine serotypes, indicating the ongoing presence of vaccine serotypes in young children. The qPCR method was more sensitive in detecting S. pneumoniae and serotypes compared to traditional culture and Quellung reaction methods.
Article
Biochemical Research Methods
Ian Dardani, Benjamin L. Emert, Yogesh Goyal, Connie L. Jiang, Amanpreet Kaur, Jasmine Lee, Sara H. Rouhanifard, Gretchen M. Alicea, Mitchell E. Fane, Min Xiao, Meenhard Herlyn, Ashani T. Weeraratna, Arjun Raj
Summary: clampFISH 2.0 is an efficient method for detecting multiple RNA species and amplifying signals simultaneously, enabling the detection of 10 different RNA species in over 1 million cells and also applicable to tissue sections.
Article
Biochemistry & Molecular Biology
Mei Yang, Ruihua Ji, Zhengqing Zhao, Wenwen Wang, Ye Lu, Zhenghua Xiang, Hongbin Yuan
Summary: This study established a new method called In Situ Hybridization Chain Reaction (isHCR) for in situ detection of apoptotic DNA fragments. Compared to the current TUNEL method, isHCR can specifically detect DNA fragmentation in the cytoplasm of early-stage apoptotic cells, with low cost and the ability to handle large numbers of specimens. This suggests that isHCR has the potential to play a role in the in situ detection of DNA fragmentation in apoptotic cells.
Article
Chemistry, Analytical
Jianhui Xu, Ruijin Zeng, Lingting Huang, Zhenli Qiu, Dianping Tang
Summary: A magnetic-assisted platform was established for monitoring prostate-specific antigen (PSA), based on Bi2MoO6 nanosheets (BMO) and coupling the aptamer-guided hybridization chain reaction (HCR) with the hydrolysate-induced vulcanization reaction. The platform showed high precision with a quantitative diagnosis sensitivity area in a range of 5.0 pg mL(-1)-100 ng mL(-1).
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Bowen Zhang, Taoran Tian, Dexuan Xiao, Shaojingya Gao, Xiaoxiao Cai, Yunfeng Lin
Summary: A DNA-enhanced HCR detection system (T-probe system) is introduced for the detection of cancer-related targets, demonstrating its versatility and efficiency by detecting miRNA-21 and nucleolin. The T-probe system shows high detection rate and rapid recognition of both membrane and intracellular cancer-related targets, and exhibits superior fluorescence for imaging tumor-related biomarkers in vitro and in vivo, indicating its great potential for clinical diagnosis and therapeutic applications.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Infectious Diseases
Francesca De Falco, Federica Corrado, Anna Cutarelli, Leonardo Leonardi, Sante Roperto
Summary: This study compared the levels of BPV DNA in bovine samples using ddPCR and qPCR, finding that ddPCR was more sensitive and accurate than qPCR, allowing the detection of more BPV genotypes.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Article
Biochemical Research Methods
Shaolei Huang, Yiquan An, Bangchao Xi, Xianglian Gong, Zhongfu Chen, Shan Shao, Shengxiang Ge, Jun Zhang, Dongxu Zhang, Ningshao Xia
Summary: Nucleic acid detection is a method that directly identifies the presence of pathogenic microorganisms and has advantages of high sensitivity, commendable specificity, and a short window period. A real-time PCR system based on multiple temperature zones was proposed to solve the time-consuming problem, enabling fast detection speed and high sensitivity under extreme conditions.
Article
Biotechnology & Applied Microbiology
Suresh Reddy Banda, Holger Klapproth, Nicolaas Smit, Sonja Bednar, Thomas Brandstetter, Juergen Ruehe
Summary: The sensitivity of a PCR-based biochip assay depends on the efficiency of PCR amplicons in binding to the microarray spots. Asymmetric PCR is often inefficient in generating single-stranded (ss) amplicons. However, a novel variant called Asymmetric Exponential and Linear Amplification (AELA) can overcome these issues and generate large amounts of ss-amplicons. AELA-PCR combines both exponential and linear amplification of the target nucleic acid, resulting in higher biochip sensitivity compared to conventional methods.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Virology
Selin Unsaler, Ayse Okan, Suda Tekin, Aysenur M. Hafiz, Ozan Gokler, Ozan Altuntas
Summary: The study aimed to compare RT-PCR results of NA and NS samples in the diagnosis of COVID-19. The results showed no statistical difference in Ct values between NA and NS samples, indicating similar effectiveness of the two sampling methods in detecting the coronavirus.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Clinical Neurology
Jack Phan, Courtney Pollard, Paul D. Brown, Nandita Guha-Thakurta, Adam S. Garden, David I. Rosenthal, Clifton D. Fuller, Steven J. Frank, G. Brandon Gunn, William H. Morrison, Jennifer C. Ho, Jing Li, Amol J. Ghia, James N. Yang, Dershan Luo, He C. Wang, Shirley Y. Su, Shaan M. Raza, Paul W. Gidley, Ehab Y. Hanna, Franco DeMonte
JOURNAL OF NEUROSURGERY
(2019)
Review
Otorhinolaryngology
Fernando Lopez, Carlos Suarez, Vincent Vander Poorten, Antti Makitie, Iain J. Nixon, Primoz Strojan, Ehab Y. Hanna, Juan Pablo Rodrigo, Remco de Bree, Miquel Quer, Robert P. Takes, Carol R. Bradford, Ashok R. Shaha, Alvaro Sanabria, Alessandra Rinaldo, Alfio Ferlito
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
(2019)
Article
Otorhinolaryngology
Ashley C. Mays, Ehab Y. Hanna, Renata Ferrarotto, Jack Phan, Diana Bell, Natalie Silver, Collin F. Mulcahy, Dianna Roberts, Ahmed Salama Ahmed Abdelmeguid, Clifton D. Fuller, Steven J. Frank, Shaan M. Raza, Michael E. Kupferman, Franco DeMonte, Shirley Y. Su
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
(2018)
Article
Otorhinolaryngology
Karim W. Asi, Ahmed Abdelmeguid, Diana Bell, Ehab Y. Hanna
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
(2018)
Meeting Abstract
Oncology
H. Bahig, E. Y. Hanna, A. S. Garden, S. P. Ng, T. Nguyen, S. J. Frank, G. B. Gunn, D. I. Rosenthal, C. D. Fuller, W. H. Morrison, R. Ferrarotto, D. Bell, S. Y. Su, J. Phan
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2018)
Meeting Abstract
Oncology
H. Bahig, S. P. Ng, C. Pollard, T. Nguyen, G. B. Gunn, D. I. Rosenthal, C. D. Fuller, S. J. Frank, A. S. Garden, J. Reddy, W. H. Morrison, E. Y. Hanna, F. DeMonte, S. Y. Su, J. Phan
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2018)
Meeting Abstract
Oncology
P. Aggarwal, J. Zaveri, R. P. Goepfert, G. B. Gunn, S. Lai, C. D. Fuller, E. Y. Hanna, D. I. Rosenthal, J. S. Lewin, K. A. Hutcheson
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2018)
Article
Oncology
Moran Amit, Ahmed S. Abdelmeguid, Teemaranawich Watcherporn, Hideaki Takahashi, Samantha Tam, Diana Bell, Renata Ferrarotto, Bonnie Glisson, Michael E. Kupferman, Dianna B. Roberts, Shirley Y. Sue, Shaan M. Raza, Franco DeMonte, Ehab Y. Hanna
JOURNAL OF CLINICAL ONCOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Rodabe N. Amaria, Sangeetha M. Reddy, Hussein A. Tawbi, Michael A. Davies, Merrick Ross, Isabella C. Glitza, Janice N. Cormier, Carol Lewis, Wen-Jen Hwu, Ehab Hanna, Adi Diab, Michael K. Wong, Richard Royal, Neil Gross, Randal Weber, Stephen Y. Lai, Richard Ehlers, Jorge Blando, Denai R. Milton, Scott Woodman, Robin Kageyama, Daniel K. Wells, Patrick Hwu, Sapna P. Patel, Anthony Lucci, Amy Hessel, Jeffrey E. Lee, Jeffrey Gershenwald, Lauren Simpson, Elizabeth M. Burton, Liberty Posada, Lauren Haydu, Linghua Wang, Shaojun Zhang, Alexanderj Lazar, Courtney W. Hudgens, Vancheswaran Gopalakrishnan, Alexandre Reuben, Miles C. Andrews, Christine N. Spencer, Victor Prieto, Padmanee Sharma, James Allison, Michael T. Tetzlaff, Jennifer A. Wargo
Review
Otorhinolaryngology
Mohsin Shah, Mona K. Jomaa, Renata Ferrarotto, Sai-Ching J. Yeung, Ehab Y. Hanna, Cielito C. Reyes-Gibby
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
(2019)
Article
Oncology
Dario Pasalic, Ethan B. Ludmir, Pamela K. Allen, Nikhil G. Thaker, Bhavana V. Chapman, Ehab Y. Hanna, Shirley Y. Su, Renata Ferrarotto, Bonnie S. Glisson, Jay P. Reddy, G. Brandon Gunn, C. David Fuller, Jack Phan, David I. Rosenthal, William H. Morrison, Adam S. Garden, Steven J. Frank
RADIOTHERAPY AND ONCOLOGY
(2020)
Article
Radiology, Nuclear Medicine & Medical Imaging
Anton S. Becker, Rocio Perez-Johnston, Sona A. Chikarmane, Melissa M. Chen, Maria El Homsi, Kimberly N. Feigin, Katherine M. Gallagher, Ehab Y. Hanna, Marshall Hicks, Ahmet T. Ilica, Erica L. Mayer, Atul B. Shinagare, Randy Yeh, Marius E. Mayerhoefer, Hedvig Hricak, H. Alberto Vargas
Summary: Vaccination-associated adenopathy is a common imaging finding after COVID-19 vaccination, which may be mistaken for malignant nodal involvement in patients with cancer. Routine imaging should be scheduled at least 6 weeks after vaccination, while clinically indicated imaging should not be delayed.
Letter
Medicine, General & Internal
Ewe-Seng Ch'ng
NEW ENGLAND JOURNAL OF MEDICINE
(2020)
Meeting Abstract
Pathology
Franco DeMonte, Ehab Y. Hanna, Shaan M. Raza, Achim H. Bell, Victor Prieto, Gregory Fuller, Diana Bell
Article
Clinical Neurology
Jacob L. Freeman, Franco DeMonte, Wajd Al-Holou, Paul W. Gidley, Ehab Y. Hanna, Michael E. Kupferman, Shirley Y. Su, Shaan M. Raza
ACTA NEUROCHIRURGICA
(2018)