Article
Polymer Science
Thomas Kerr-Phillips, Mona Damavandi, Lisa Pilkington, Kathryn A. Whitehead, Jadranka Travas-Sejdic, David Barker
Summary: The conformation of a fluorescent polymer is crucial in determining its fluorescent properties. This study investigated the effect of grafted polymeric side chains on the photoluminescent properties of poly(para-phenylene vinylene) (PPV) and poly(para-phenylene ethynylene) (PPE). Ionic grafts were found to decrease the quantum yield, contrary to expectations, while uncharged side chain grafts significantly increased the quantum yield. These results highlight the importance of molecular conformation in the photoluminescent properties of fluorescent polymers.
Article
Chemistry, Physical
Matthias Brosz, Nicholas Michelarakis, Uwe H. F. Bunz, Camilo Aponte-Santamaria, Frauke Graeter
Summary: Poly(para-phenylene ethynylene)s (PPEs) are a class of conjugated and semi-flexible polymers with a wide range of technological applications. In this study, a coarse-grained model for PPEs was developed using the Martini 3 force field to study large-scale assemblies computationally. The model successfully reproduced key structural and thermodynamic properties of single PPE chains and mixtures. It was found that chain entanglement increased with PPE chain length, while nematic ordering decreased. The Martini 3 PPE model allows for investigation of the structural organization of PPE bulk systems at large spatio-temporal scales and is predicted to be applicable for studying out-of-equilibrium behavior under mechanical force.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)
Article
Engineering, Biomedical
Hye-Ryoung Kim, Hui Bang Cho, Sujeong Lee, Ji -In Park, Hye Jin Kim, Keun-Hong Park
Summary: A transfer strategy based on membrane fusion called fusogenic mitochondrial capsules (FMCs) was established to effectively deliver mitochondria (MT) for the treatment of human diseases. FMCs showed faster and more efficient delivery to chondrocytes compared to naked MT, and fusion was a more stable delivery method. In vitro and in vivo experiments demonstrated that FMCs reduced inflammation, increased extracellular matrix components, and promoted cartilage regeneration.
Article
Nanoscience & Nanotechnology
Megan A. Laune, Syed Ahad Zahidi, Jared T. Wiemann, Yan Yu
Summary: Nanosized liposomes can enhance the potency of antibiotics by fusing with bacterial membranes, but the interaction mechanisms are poorly understood. The fusion interactions between cationic liposomes and Escherichia coli were found to be heterogeneous, resulting in different antibacterial outcomes.
ACS APPLIED NANO MATERIALS
(2022)
Article
Polymer Science
Jiucheng Nie, Xiaoyu Huang, Guolin Lu, Mitchell A. Winnik, Chun Feng
Summary: This study investigates the influence of different architectures of pi-conjugated blocks on the crystallization-driven self-assembly (CDSA) of BCPs. The results show that different block architectures can lead to different aggregate morphologies. Furthermore, experiments on heterogeneous seeded growth reveal differences in the behavior of BCPs with different architectures.
Article
Multidisciplinary Sciences
Lining Zheng, Sarith R. Bandara, Zhengzhong Tan, Cecilia Leal
Summary: RNA therapeutics show potential in treating genetic diseases. Lipid nanoparticles (LNPs) are effective RNA delivery systems, but improving their design is crucial for treating more diseases with high potency, specific targeting, and low side effects. Overcoming the challenge of releasing cargo from LNPs after endocytosis is a significant hurdle.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Multidisciplinary
Shi-Jie Hao, Ya-Xuan Zhu, Fu -Gen Wu
Summary: Researchers developed a membrane fusion liposome (MFL) loaded with a small-molecule tyrosine kinase inhibitor (TKI) and doxorubicin (Dox) to achieve tumor cell membrane fusion-mediated drug delivery and enhanced chemotherapy of drug-resistant tumor.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Biochemistry & Molecular Biology
Natalia Voskoboynikova, Eleonora Germana Margheritis, Felix Kodde, Malte Rademacher, Maurice Schowe, Annette Budke-Gieseking, Olympia-Ekaterini Psathaki, Heinz-Jurgen Steinhoff, Katia Cosentino
Summary: Amphiphilic maleic acid-containing polymers allow for the direct extraction of membrane proteins into stable, homogenous, water-soluble copolymer/lipid nanoparticles without the use of detergents. However, an increase in the size of the lipid nanoparticles may correlate with increased sample heterogeneity, thus hampering their application to spectroscopic and structural techniques where highly homogeneous samples are desirable.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2021)
Article
Chemistry, Multidisciplinary
Ye Zeng, Mengjie Shen, Ankush Singhal, Geert Jan Agur Sevink, Niek Crone, Aimee L. L. Boyle, Alexander Kros
Summary: An ideal nanomedicine system improves drug therapy by enhancing cellular uptake. However, current nanomedicines mainly enter cells through endosomal/lysosomal pathways, leading to inefficient delivery of therapeutic cargo. To overcome this issue, the authors developed a synthetic lipidated peptide pair, E4/K4, inspired by natural fusion machinery. By optimizing the structure of the peptide, the researchers achieved enhanced membrane fusion and efficient delivery of drugs into cells. This study provides valuable insights for the development of efficient drug delivery systems based on liposome-cell fusion strategies.
Article
Chemistry, Multidisciplinary
Xinwei Tian, Nikolaj Alexander Risgaard, Philipp M. G. Loffler, Stefan Vogel
Summary: Lipid nanoreactors are biomimetic reaction vessels that can host chemical and enzymatic reactions. This study presents DNA-programmed lipid nanoreactors (PLNs) functionalized with lipidated oligonucleotides (LiNAs) for the encoding and programming of reactions. The PLN method offers a platform for synthesis in confined space, allowing for the generation of combinatorial libraries and analysis of the molecules formed.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Xinwei Tian, Nikolaj Alexander Risgaard, Philipp M. G. Loffler, Stefan Vogel
Summary: Lipid nanoreactors are biomimetic reaction vessels that can host various chemical and enzymatic reactions. By using DNA-programmed lipid nanoreactors (PLNs), reactions can be encoded and controlled through membrane fusion, allowing for the synthesis of a range of carbohydrate mimetics. This method offers a robust platform for confined space synthesis and the generation of combinatorial libraries.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemistry & Molecular Biology
Philipp Grad, Katarina Edwards, Lars Gedda, Victor Agmo Hernandez
Summary: This study investigates the effects of polyethylene glycol-(PEG) modified lipids and gangliosides on the Ca2+ induced interaction between liposomes composed of palmitoyl-oleoyl phosphatidylethanolamine (POPE) and palmitoyl-oleoyl phosphatidylserine (POPS) at physiological ionic strength. The results show that naked liposomes tend to adhere, rupture, and collapse on each other's surfaces upon addition of Ca2+, eventually resulting in the formation of large multilamellar aggregates and bilayer sheets. However, the presence of gangliosides or PEGylated lipids leads to the formation of small, long-lived bilayer fragments/disks. PEGylated lipids seem to be more effective than gangliosides at stabilizing these structures. The study suggests that direct liposome-liposome fusion is not the dominating process triggered by Ca2+ in the systems studied.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Joerg Nikolaus, Kasey Hancock, Maria Tsemperouli, David Baddeley, Erdem Karatekin
Summary: Researchers used a polarization-controlled total internal reflection fluorescence (pTIRF) microscope to monitor the fusion of proteoliposomes with planar lipid bilayers, investigating membrane fusion pore dynamics. By varying excitation polarization angle, they identified a dye-dependent optimal polarization for maximal fluorescence increase upon fusion, aiding event detection and lipid transfer kinetics analysis.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Biochemistry & Molecular Biology
K. S. Trosheva, S. A. Sorokina, A. A. Efimova, P. Semenyuk, A. K. Berkovich, A. A. Yaroslavov, Z. B. Shifrina
Summary: Dendrimers exhibit electrostatic and reversible interaction with liposomes, with only a portion of pyridinium groups being involved in binding. Additionally, dendrimer molecules have the ability to migrate between liposomes. The phase state of the lipid membrane and the presence of cholesterol do not affect the formation mechanism of dendrimer-liposome complexes, paving the way for potential biological applications of pyridylphenylene dendrimers.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2021)
Article
Chemistry, Multidisciplinary
Jia Shi, Taisei Yashiro, Masahiro Teraguchi, Takashi Kaneko, Toshiki Aoki
Summary: In this study, a series of fully conjugated polymers with a rigid backbone consisting of (di)ethynylene and phenylene groups and alkyl side groups were synthesized by Glaser or Sonogashira coupling polycondensation. Some of these polymers formed self-supporting membranes, and their oxygen permselectivities were found to be influenced by the alkyl groups. The oxygen permselectivities of polymers containing diynes in the main chain were determined for the first time.
Editorial Material
Chemistry, Multidisciplinary
Krishna N. Ganesh, Marc Hillmyer, David Kaplan, Sebastien Lecommandoux, Jodie L. Lutkenhaus, Stuart Rowan, Kirk Schanze, Shu Wang, Deqing Zhang
Review
Nanoscience & Nanotechnology
Han Sun, Kirk S. Schanze
Summary: This article comprehensively reviews the synthetic chemistries used to covalently link biofunctional groups onto water-soluble conjugated polymer platforms, including different types of backbones. It also discusses the applications and challenges of functionalized conjugated polymers.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Editorial Material
Nanoscience & Nanotechnology
Kirk S. Schanze, Laura E. Fernandez, Shu Wan, T. Randall Lee
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Nanoscience & Nanotechnology
Han Sun, Samantha S. Yee, Habtom B. Gobeze, Ru He, Daniel Martinez, April L. Risinger, Kirk S. Schanze
Summary: We report a water-soluble polymer that can be used for photoactivated chemotherapy. The polymer undergoes electron transfer upon light activation, resulting in the release of an anticancer drug and cytotoxicity towards cancer cells.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Editorial Material
Nanoscience & Nanotechnology
Cong Li, Chunhai Fan, Kirk S. Schanze, Laura Fernandez
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Aya Sakaya, Isabel O. L. Bacellar, Jose Luis Fonseca, Andres M. Durantini, Julia McCain, Laiyi Xu, Mariana Vignoni, Andres H. Thomas, Mauricio S. Baptista, Gonzalo Cosa
Summary: A sensitive fluorogenic probe is developed to quantify the arrival of singlet oxygen (1O2) on lipid membranes, allowing for the study of membrane dynamics. The probe shows remarkable intensity enhancement upon 1O2 trapping and enables the visualization of membrane expansion dynamics.
Editorial Material
Nanoscience & Nanotechnology
Kirk Schanze, Christy L. Haynes, Kirk S. Schanze, Laura E. Fernandez
ACS APPLIED MATERIALS & INTERFACES
(2023)
Editorial Material
Chemistry, Multidisciplinary
Jillian M. Buriak, Deji Akinwande, Natalie Artzi, C. Jeffrey Brinker, Cynthia Burrows, Warren C. W. Chan, Chunying Chen, Xiaodong Chen, Manish Chhowalla, Lifeng Chi, William Chueh, Cathleen M. Crudden, Dino Di Carlo, Sharon C. Glotzer, Mark C. Hersam, Dean Ho, Tony Y. Hu, Jiaxing Huang, Ali Javey, Prashant V. Kamat, Il-Doo Kim, Nicholas A. Kotov, T. Randall Lee, Young Hee Lee, Yan Li, Luis M. Liz-Marzan, Paul Mulvaney, Prineha Narang, Peter Nordlander, Rahmi Oklu, Wolfgang J. Parak, Andrey L. Rogach, Mathieu Salanne, Paolo Samori, Raymond E. Schaak, Kirk S. Schanze, Tsuyoshi Sekitani, Sara Skrabalak, Ajay K. Sood, Ilja K. Voets, Shu Wang, Shutao Wang, Andrew T. S. Wee, Jinhua Ye
Article
Chemistry, Physical
Shorooq A. Alomar, Luis Gutierrez-Arzaluz, Issatay Nadinov, Ru He, Xiaodan Wang, Jian-Xin Wang, Jiangtao Jia, Osama Shekhah, Mohamed Eddaoudi, Husam N. Alshareef, Kirk S. Schanze, Omar F. Mohammed
Summary: Structural modifications to molecular systems that control photon emission processes are crucial for the development of fluorescence sensors, X-ray imaging scintillators, and OLEDs. This study explored the effects of slight changes in chemical structure on interfacial excited-state transfer processes using two donor-acceptor systems. The results showed efficient energy transfer in the SDZ-TADF system and both energy and electron transfer processes in the Ac-SDZ-TADF system. Transient absorption measurements and TD-DFT calculations confirmed the occurrence of photoinduced electron transfer in the Ac-SDZ-TADF system. This work provides a straightforward approach to modulate excited-state energy/charge transfer processes at donor-acceptor interfaces.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Article
Biochemistry & Molecular Biology
Zhiliang Li, Charles J. Zeman, Silvano Valandro, Jose Paolo O. Bantang, Kirk S. Schanze
Summary: Non-covalent interactions are widely used in supramolecular chemistry to create nanoscale structures. However, the biomimetic self-assembly and reversibility of diverse nanostructures induced by different important biomolecules in aqueous solution remain challenging.
Article
Chemistry, Multidisciplinary
Taemin Park, Minwoo Han, Kirk S. S. Schanze, Seoung Ho Lee
Summary: We developed a fluorescence sensing strategy based on a self-assembled amphiphilic pyrene/protamine complex system for sensitive and selective detection of trypsin in human urine, which is important for evaluating pancreatic disease. The fluorescence of functionalized pyrene aggregates in aqueous solution was quenched by protamine, but this quenched fluorescence could be recovered by the enzymatic reaction of trypsin. This sensing platform successfully detected trypsin at low concentrations and screened for trypsin inhibitors in non-pretreated human urine.
Article
Nanoscience & Nanotechnology
Michael D. Turlington, Habtom B. Gobeze, Muhammed Younus, Kirk S. Schanze
Summary: A study on the wavelength-dependent interfacial electron transfer mechanisms of diblock oligomers containing oligothiophene and 4,7-di(thiophen-2-yl)benzo[c][1,2,5]thiadizole segments anchored to mesoporous TiO2 films was conducted. It was found that the injection pathway and recombination pathway were influenced by the excitation wavelength and the bridging unit between the blocks. Control over hole localization in the electron transfer process could be achieved by varying the excitation wavelength.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Editorial Material
Engineering, Electrical & Electronic
Hyun Jae Kim, Kirk Schanze, Chengmei Zhong
ACS APPLIED ELECTRONIC MATERIALS
(2023)
Article
Chemistry, Physical
Habtom B. Gobeze, Pradeepkumar Jagadesan, Kirk S. Schanze
Summary: This paper investigates the electronic structure and photophysical properties of two diblock π-conjugated oligomers with electron rich tetra(thiophene) (T4) or octa(thiophene) (T8) segments linked to an electron poor 4,7-bis(2-thienyl)-2,1,3-benzothiadiazole (TBT) moiety. The results show that the diblock oligomers display redox and absorption features that can be attributed to the Tn and TBT units, and the frontier orbitals on the diblock oligomers retain characteristics of the individual π-conjugated segments. Moreover, the fluorescence and transient absorption spectroscopy reveal that the photophysical properties and dynamics of the excited states are dominated by charge transfer between the oligo-thiophene and TBT units.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Chemistry, Inorganic & Nuclear
Amran A. T. Khan, Habtom B. Gobeze, Tanjila Islam, Hadi D. Arman, Kirk S. Schanze
Summary: This study investigates the effect of bromine substitution on the trans-(NHC)(2)Pt-II(CC-Ar)(2) chromophore through photophysical studies. The results show that the mono-bromo complex has lower emission quantum yield and lifetime, attributed to a larger non-radiative decay rate.
DALTON TRANSACTIONS
(2023)