Article
Chemistry, Multidisciplinary
Roman A. Voloshin, Ekaterina S. Lokteva, Suleyman I. Allakhverdiev
Summary: Biohybrid devices based on natural pigment-protein complexes are being widely studied, with a focus on designing electrodes using photo-system I. This review emphasizes the benefits of nanostructured electrodes, recombinant photosystems, and electrophoretic deposition techniques for uniform photosystem I orientation on an electrode.
CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY
(2023)
Article
Chemistry, Physical
Elisabeth Hengge, Markus Hirber, Philipp Brunner, Eva-Maria Steyskal, Bernd Nidetzky, Roland Wuerschum
Summary: The electrochemical behavior of nanoporous gold modified with self-assembled monolayers was investigated, with long-chain MHDA showing the most distinct point of zero charge for precise charge control. Proton transfer reaction studies indicated an increased fraction of electrochemically controllable SAMs compared to planar electrodes, suggesting attractive application potential.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2021)
Article
Chemistry, Physical
Rajendra P. Gautam, Christopher J. Barile
Summary: The study reveals that on carbon electrodes, electron transfer between modified SAMs and ferrocene molecules may be driven by defect sites, rather than the alkyl linker tunneling mechanism. Additionally, blocking SAM defect sites with ZnO electrodeposits can alter the electron transfer rates.
JOURNAL OF PHYSICAL CHEMISTRY C
(2021)
Article
Nanoscience & Nanotechnology
Ravi Pamu, Benjamin J. Lawrie, Bamin Khomami, Dibyendu Mukherjee
Summary: The study investigated wavelength-dependent plasmon-enhanced photocurrents from PSI immobilized around highly ordered Au (AuND) and Ag (AgND) nanodisks. The results showed significant enhancements in photocurrents at specific excitation wavelengths, paving the way for rational assembly of future biohybrid structures.
ACS APPLIED NANO MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
H. Doerpholz, S. Subramanian, A. Zouni, F. Lisdat
Summary: In this study, photobioelectrodes based on a ferredoxin-modified photosystem I (PSI-Fd) from Thermosynechococcus vestitus were prepared and characterized. The direct electron transfer between PSI-Fd and the electrode was investigated. The results showed that the modified PSI-Fd photobioelectrode had a lower photocurrent and a lower onset potential of the cathodic photocurrent compared to native PSI, mainly due to the different adsorption behavior of the PSI-Fd construct onto the 3D ITO electrode. However, the overall behavior was similar to PSI. First experiments demonstrated the usage of the PSI-Fd photobioelectrode for enzyme-driven NADPH generation.
BIOELECTROCHEMISTRY
(2023)
Article
Physics, Multidisciplinary
Tomas Opatrny, Avijit Misra, Gershon Kurizki
Summary: The concept of extracting work from thermal noise through phase-sensitive measurements and unitary manipulations is proposed, showing improved efficiency and power with greater average quantum input. This protocol proves to be advantageous compared to traditional models of information and heat engines.
PHYSICAL REVIEW LETTERS
(2021)
Article
Chemistry, Physical
Yusuke Yoneda, Masaya Kito, Daiki Mori, Akari Goto, Masaharu Kondo, Hiroshi Miyasaka, Yutaka Nagasawa, Takehisa Dewa
Summary: In this study, a biohybrid LH system was constructed, achieving efficient energy transfer through non-covalent self-assembly. The results suggest that this facile self-assembled biohybrid LH system holds great promise for solar-energy conversion.
JOURNAL OF CHEMICAL PHYSICS
(2022)
Review
Chemistry, Multidisciplinary
Andrew J. Bagnall, Sagar Ganguli, Alina Sekretareva
Summary: This review focuses on the mechanisms behind photocurrents observed in plasmon-enhanced electrocatalysis (PEEC) and critically evaluates alternative sources of current enhancement. Guidelines for experimentalists to distinguish between different enhancement mechanisms in PEEC are presented.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Green & Sustainable Science & Technology
Yuriy Shapovalov, Rustam Tokpayev, Tamina Khavaza, Mikhail Nauryzbayev
Summary: Photosynthesis is considered a promising area for cheap and environmentally friendly energy production. The process involves water oxidation to generate oxygen and hydrogen, with the mechanisms of H+, O-2 up arrow, e(-) production described in this review. The functioning mechanisms of PS-I and PS-II can potentially be used in developing environmentally friendly technologies for molecular hydrogen production.
Article
Chemistry, Physical
Dimitrios Stefanakis, Vangelis Harmandaris, Georgios Kopidakis, Ioannis Remediakis
Summary: The study examines the impact of substrate on the structural properties and morphology of alkanethiol self-assembled monolayers on gold surfaces through calculations and simulations. Changes in substrate lead to transitions from order to disorder, with substrate morphology playing a crucial role in determining long-range order.
JOURNAL OF PHYSICAL CHEMISTRY C
(2021)
Article
Engineering, Electrical & Electronic
Akanksha Singh, Sarthak Mandal, Shaojiang Chen, Minghui Liu, Christopher J. Gisriel, Anne-Marie Carey, Hao Yan, Dong-Kyun Seo, Su Lin, Neal W. Woodbury
Summary: A biohybrid electronic system has been developed with a photosystem I (PSI) fixed on a porous antimony-doped tin oxide electrode, resulting in light-powered reduction of NADPH. Cytochrome c was used to orient PSI reaction centers and transport electrons, generating a photocurrent. Despite attempts to catalyze dye reduction by NADPH, dye reduction occurred even without enzymes, with direct light-dependent reduction of NADPH and subsequent reduction of resazurin.
ACS APPLIED ELECTRONIC MATERIALS
(2021)
Article
Energy & Fuels
Emre Cevik, Muhammad Hassan, Mohammed Ashraf Gondal
Summary: In this study, a millimeter-scale bioelectrochemical cell was fabricated using a thylakoid membrane on a screen-printed electrode to generate direct and mediated photocurrent without using a selective membrane. Significant enhancement in photocurrent generation was observed when an efficient amount of mediator was used in the system.
Article
Nanoscience & Nanotechnology
Tea-Yon Kim, Byung Su Kim, Jong Gyu Oh, Seul Chan Park, Jaeyoung Jang, Thomas W. Hamann, Young Soo Kang, Jin Ho Bang, Sixto Gimenez, Yong Soo Kang
Summary: By applying a TiO2(B) surface passivation layer (SPL) on TiO2/QD (PbS and CdS), electron conductivity was improved, collection efficiency was increased, and a record high photocurrent density was achieved, suggesting a promising strategy for the development of high-performance photoelectrochemical devices.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Analytical
Marcin Gwiazda, Sheetal K. Bhardwaj, Ewa Kijenska-Gawronska, Wojciech Swieszkowski, Unni Sivasankaran, Ajeet Kaushik
Summary: This research presents an electrochemical immunosensor for collagen I detection using stable covalent immobilization of gold nanoparticles and enzymatically reduced antibodies, achieving high sensitivity in the range of pg/mL. The sensor's unique advantage lies in the appropriate orientation of antibodies onto the surface, good stability, and selectivity properties, with a detection range of 1 to 5 pg/mL.
Article
Biochemistry & Molecular Biology
Tom Schwartz, Mariia Fadeeva, Daniel Klaiman, Nathan Nelson
Summary: The mutant TSP9 of Chlamydomonas reinhardtii, isolated through negative selection of UV irradiation-mutated cells, has a single amino acid mutation in the Rieske protein. It loses its function at 37 degrees C but recovers at 25 degrees C. The mutant showed structural alterations in photosystem I and II supercomplexes, suggesting a possible response mechanism involving the exclusion of certain subunits.
Review
Cell Biology
Madeline M. Davis, Rajan Lamichhane, Barry D. Bruce
Summary: Translocons are protein assemblies that aid in protein targeting and transport across biological membranes. Recent advancements in cryogenic electron microscopy and single-molecule fluorescence microscopy have allowed for a more detailed understanding of translocon function. These methods are crucial in studying the structure, function, and dynamics of translocons.
TRENDS IN CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nathan G. Brady, Cameron E. Workman, Bridgie Cawthon, Barry D. Bruce, Brian K. Long
Summary: This study demonstrates that functionalizing SMAs can enhance the efficiency of extracting PSI from cyanobacterial membranes, with the number and length of side chains playing a crucial role in the extraction mechanism of PSI.
Article
Engineering, Biomedical
Alexandra H. Teodor, Lucas B. Thal, Shinduri Vijayakumar, Madison Chan, Gabriela Little, Barry D. Bruce
Summary: The study explores the encapsulation of detergent-solubilized PSI in CaCO3 microspheres, producing uniform, monodisperse, mesoporous microspheres. This method proves to be effective in enhancing protein stability and activity, particularly for long-term storage of large membrane proteins.
MATERIALS TODAY BIO
(2021)
Article
Chemistry, Multidisciplinary
Diandra Doppler, Mohammad T. Rabbani, Romain Letrun, Jorvani Cruz Villarreal, Dai Hyun Kim, Sahir Gandhi, Ana Egatz-Gomez, Mukul Sonker, Joe Chen, Faisal H. M. Koua, Jayhow Yang, Mohamed Youssef, Victoria Mazalova, Sasa Bajt, Megan L. Shelby, Matt A. Coleman, Max O. Wiedorn, Juraj Knoska, Silvan Schon, Tokushi Sato, Mark S. Hunter, Ahmad Hosseinizadeh, Christopher Kuptiz, Reza Nazari, Roberto C. Alvarez, Konstantinos Karpos, Sahba Zaare, Zachary Dobson, Erin Discianno, Shangji Zhang, James D. Zook, Johan Bielecki, Raphael de Wijn, Adam R. Round, Patrik Vagovic, Marco Kloos, Mohammad Vakili, Gihan K. Ketawala, Natasha E. Stander, Tien L. Olson, Katherine Morin, Jyotirmory Mondal, Jonathan Nguyen, Jose Domingo Meza-Aguilar, Gerdenis Kodis, Sara Vaiana, Jose M. Martin-Garcia, Valerio Mariani, Peter Schwander, Marius Schmidt, Marc Messerschmidt, Abbas Ourmazd, Nadia Zatsepin, Uwe Weierstall, Barry D. Bruce, Adrian P. Mancuso, Thomas Grant, Anton Barty, Henry N. Chapman, Matthias Frank, Raimund Fromme, John C. H. Spence, Sabine Botha, Petra Fromme, Richard A. Kirian, Alexandra Ros
Summary: This study proposes a new method to address the issue of crystal introduction in the X-ray beam. By using an inert oil phase to supplement the flow of the sample, stable injection of crystals from two different materials can be achieved, avoiding crystal clogging and potentially increasing the preservation rate of crystal samples, enabling degradation-free light-induced time-resolved SFX.
JOURNAL OF APPLIED CRYSTALLOGRAPHY
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Yue Zhou, Barry Bruce, Todd Reynolds
Article
Biochemistry & Molecular Biology
Alexandra H. Teodor, Stephanie Monge, Dariana Aguilar, Alexandra Tames, Roger Nunez, Elaine Gonzalez, Juan J. Montero Rodriguez, Jesse J. Bergkamp, Ricardo Starbird, Venkatesan Renugopalakrishnan, Barry D. Bruce, Claudia Villarreal
Summary: This study explores a novel counter electrode and redox mediator system that can be used for both PSI and bR devices and performs well. The compatibility of disparate photosensitizers with common mediators and counter electrodes may improve bio-sensitized PV design.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Nathan G. Brady, Shuo Qian, Jon Nguyen, Hugh M. O'Neill, Barry D. Bruce
Summary: This study utilized SANS technique to investigate the geometry of SMALPs and reported the dimensions of the Thermosynechococcus elongatus PSI-SMALP nanodiscs. The results showed that PSI-SMALP is the largest isolated SMALP to date, and its lipid composition differs significantly from the bulk thylakoid composition.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2022)
Article
Biochemistry & Molecular Biology
Cameron E. Workman, Bridgie Cawthon, Nathan G. Brady, Barry D. Bruce, Brian K. Long
Summary: The detergent-free extraction of integral membrane proteins using styrene-maleic acid copolymers (SMAs) is a promising technique for isolating proteins in a more native-like conformation. However, the monoesterification of SMAs with alkoxy ethoxylate sidechains can significantly affect the bioactivity of these copolymers in protein extraction. Furthermore, the preparation, purification, and storage conditions of these esterified SMA analogues can also alter their structure and impact the protein extraction process.
Article
Biochemistry & Molecular Biology
Yue Zhou, Jawhar H. Syed, Dmitry A. Semchonok, Edward Wright, Fotis L. Kyrilis, Farzad Hamdi, Panagiotis L. Kastritis, Barry D. Bruce, Todd B. Reynolds
Summary: Phosphatidylserine (PS) synthase encoded by the CHO1 gene is a potential drug target for antifungals against systemic candidiasis. This study investigated methods to solubilize and purify Cho1 protein, which has a transmembrane nature. The results showed that digitonin or DDM were the most effective detergents for solubilization, and protein purification was achieved through affinity chromatography and TEV protease treatment. The purified Cho1 protein exhibited optimal enzyme activity under specific conditions of temperature, pH, and manganese and detergent concentrations, which is important for substrate determination and drug screening.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Cameron E. Workman, Pushan Bag, Bridgie Cawthon, Fidaa H. Ali, Nathan G. Brady, Barry D. Bruce, Brian K. Long
Summary: Styrene-maleic acid copolymers and related amphiphilic copolymers are promising tools for isolating and studying integral membrane proteins. A new polymeric platform is introduced in this study, which overcomes limitations of existing copolymers and offers advantages such as low UV absorbance, high protein extraction yields, and retention of native-like protein structure.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Christopher Espinoza-Araya, Ricardo Starbird, E. Senthil Prasad, Venkatesan Renugopalakrishnan, Ashok Mulchandani, Barry D. Bruce, Claudia C. Villarreal
Summary: There is a high demand for renewable and sustainable energy production worldwide. Bio-sensitized solar cells with bacteriorhodopsin (bR) as the biosensitizer show promise due to their simplicity, stability, and quantum efficiency. In this study, a mutant of bR, D96N, was utilized in a solar cell with low-cost, carbon-based components. The bR device achieved a current density of 1.0 mA/cm(2), a fill factor of similar to 24%, and a power conversion efficiency of 0.16%.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2023)
Review
Chemistry, Multidisciplinary
Roman A. Voloshin, Ayshat M. Bozieva, Barry D. Bruce, Suleyman I. Allakhverdiev
Summary: Membrane electron transfer is essential for energy conversion in central metabolic pathways. Photosynthetic and respiratory electron transport chains can generate proton gradients from sunlight or chemical energy. The use of these apparatuses as energy convertors, especially those based on photosynthesis, has gained interest due to their availability and eco-friendliness. This article reviews the latest advances and challenges in this field.
RUSSIAN CHEMICAL REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Sergei K. Zharmukhamedov, Mehriban S. Shabanova, Irada M. Huseynova, Mehmet Sayim Karacan, Nurcan Karacan, Hande Akar, Vladimir D. Kreslavski, Hesham F. Alharby, Barry D. Bruce, Suleyman I. Allakhverdiev
Summary: Modern agricultural cultivation heavily relies on genetically modified plants that can survive after exposure to herbicides. However, there is a need for new sustainable and biodegradable herbicides. We developed a novel [CuL2]Br-2 complex that inhibits photosynthetic oxygen evolution on the micromolar level. The inhibitory mechanism of [CuL2]Br-2 remains unclear.
Article
Biochemistry & Molecular Biology
Dmitry A. Semchonok, Jyotirmoy Mondal, Connor J. Cooper, Katrina Schlum, Meng Li, Muhamed Amin, Carlos O. S. Sorzano, Erney Ramirez-Aportela, Panagiotis L. Kastritis, Egbert J. Boekema, Albert Guskov, Barry D. Bruce
Summary: The tetrameric structure of photosystem I in cyanobacteria has been studied, showing that the tetramer is arranged through two different interfaces. The localization of chlorophyll molecules allows for excitation energy transfer within and between monomers. Tetrameric PSI may play a significant role in the evolution of photosynthetic organisms.
PLANT COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Alexandra H. Teodor, Eu-Jee Ooi, Jackeline Medina, Miguel Alarcon, Michael D. Vaughn, Barry D. Bruce, Jesse J. Bergkamp
Summary: This study characterizes synthetic cobalt complexes as redox mediators for PSI-based biophotovoltaic devices and demonstrates their stability and effectiveness in organic solvents. The research shows evidence of direct electron transfer from cobalt complexes to PSI, leading to photocurrent generation. The findings suggest the potential for fine-tuning redox active species to interact with biological partners for the development of low cost, carbon-neutral energy production in the future.