Article
Chemistry, Physical
Zuhra Cinar Esin, Murat Sen
Summary: The aim of this study was to investigate the effects of radiation on the mechanical properties of platinum-cured VMQ elastomers. The results showed that radiation could increase the crosslink density of the elastomers, improving their mechanical properties, but slightly decreasing their damping properties.
RADIATION PHYSICS AND CHEMISTRY
(2023)
Article
Chemistry, Analytical
Wei Gao, Ye Wang, Feng Zhang, Sen Zhang, Hong-zhen Lian
Summary: The immobilization of tetrasulfonate calix[4]arene modified large pore mesoporous silica microspheres showed high protein adsorption efficiency and capacity, with the ability to adsorb and release proteins within a wide pH range.
Article
Chemistry, Physical
Pranab Sarker, Md Symon Jahan Sajib, Xiuping Tao, Tao Wei
Summary: This study investigates the dynamics and structure of the protein corona formed on nanoparticles, and reveals that the size and surface hydrophilicity of nanoparticles impact the formation process and structure of the protein corona. The findings enhance our understanding of the interactions between nanoparticles and biological systems.
JOURNAL OF PHYSICAL CHEMISTRY B
(2022)
Article
Biochemistry & Molecular Biology
Soraya Mohammadi, Khosro Khajeh, Majid Taghdir, Bijan Ranjbar
Summary: This paper investigates the effect of buffer and osmolytes concentrations on the thermodynamic stability of lysozyme, showing that higher concentrations can increase protein stability and lead to the formation of a molten globule-like state.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Bo Kyeong Yoon, Gamaliel Junren Ma, Hyeonjin Park, Abdul Rahim Ferhan, Nam-Joon Cho, Joshua A. Jackman
Summary: The adsorption behavior of lysozyme proteins strongly depends on the ethanol fraction in a non-monotonic fashion, influenced by competing effects of water and ethanol solvation on protein size and conformational stability. The degree of protein conformational flexibility has a greater impact on the hydrodynamic properties of lysozyme adlayers than solvation effects alone.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Environmental Sciences
Christelle Latrille, Olivier Bildstein
Summary: The study found that Ca-illite exhibited higher adsorption affinity for Cs compared to Ca-vermiculite, with non-linear adsorption behavior and multi-site exchange model playing a key role. High-selective and low-capacity sites were responsible for Cs uptake at low concentrations, while high-capacity sites dominated Cs adsorption at higher concentrations. Adsorption reversibility was demonstrated for illite at a certain range of Ca concentrations, whereas vermiculite showed partial irreversibility at higher Cs concentrations.
Article
Biochemical Research Methods
Jun Ren, Xiao Meng, Xu Xiang, Fangling Ji, Lulu Han, Xiaorong Gao, Lingyun Jia
Summary: This study developed a bioconjugation strategy using tyrosine-based dual-functional interfaces, which can efficiently capture and covalently immobilize target proteins. It has the advantages of simplicity, high efficiency, and high utilization rate of target molecules.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Chemistry, Physical
Tomas E. Benavidez, Jose D. S. Guerra, Carlos D. Garcia
Summary: The study applied dielectric spectroscopy as a simple and fast method for guiding protein adsorption experiments, demonstrating that lysozyme responds more efficiently to signals with lower frequencies. Experimental data confirmed that the highest adsorbed amounts of lysozyme were obtained at the lowest tested frequencies (0.1-1.0 Hz), in line with the corresponding dielectric features of the protein.
Article
Biochemistry & Molecular Biology
Magdalena Blachnio, Malgorzata Zienkiewicz-Strzalka, Anna Derylo-Marczewska, Liudmyla V. V. Nosach, Eugeny F. F. Voronin
Summary: A new type of composite material, biopolymer-silica materials, is proposed as selective and effective adsorbents. A new synthesis procedure using geometrical modification of silica and mechanosorption of chitosan is applied to create chitosan-nanosilica composites (ChNS) and chitosan-silica gel composites (ChSG). The adsorption efficiency of ChNS and ChSG towards anionic dyes was analyzed and the results showed that ChNS had faster adsorption kinetics due to the silica-type effect.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Applied
Bianfeng Yang, Cong Wang, Xu Ji, Xirong Yue, Guanchao Lv, Mengqi Wang
Summary: The composite adsorption material ACF-silica sol-LiCl (ASL), prepared by impregnating activated carbon fiber (ACF) with silica sol and LiCl, is proven to enhance the water vapor adsorption performance of ACF. Silica sol solidifies the soft ACF and improves adhesion area for LiCl, which effectively increases the water vapor adsorption performance. The adsorption capacity of ASL steadily increases with relative pressure and exhibits higher dynamic water absorption rate and adsorption rate coefficient than ACF.
MICROPOROUS AND MESOPOROUS MATERIALS
(2022)
Article
Engineering, Environmental
Reham Salah Hassan Boushara, Abdul Rahman Abdul Rahim, Khairiraihanna Johari, Nurul Ekmi Rabat, Amir Sada Khan, Norasikin Saman
Summary: This study investigates the adsorption behavior of dyes using silica nanocapsules (SiNCs) adsorbents and finds that toluene and ethyl ether are effective solvents for synthesizing SiNCs. SiNCs exhibit high selectivity for the removal of congo red, methyl orange, methylene blue, and Janus green B. The adsorption process follows pseudo-second-order kinetics and Langmuir isotherm model.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Environmental Sciences
Yanhua Duan, Rong Li, Kai Yu, Guangci Zeng, Chongxuan Liu
Summary: Spatial and temporal variations in groundwater arsenic concentrations are mainly influenced by changes in geochemical and hydrodynamic conditions. Competitive adsorption between arsenic and phosphate promotes arsenic desorption but some arsenic remains slowly desorbed or irreversibly sorbed in the system. Water extraction accelerates arsenic transport between layers, increasing the overall rate of arsenic release from diffusion-limited zones.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Polymer Science
Wenwen Liu, Chanjuan Zeng, Fangqing Ge, Yunjie Yin, Chaoxia Wang
Summary: The novel functionalized poly(silanization alcoholamine diacetylene)/silica nanoparticles have achieved reversible thermochromism, transitioning from blue to red at specific temperatures, and returning to bluish violet after cooling. The strategy of extending the alkyl chain length with covalent bonding promotes interaction between alkyl chains, improving the reversibility of thermochromic polydiacetylene.
JOURNAL OF APPLIED POLYMER SCIENCE
(2021)
Article
Chemistry, Analytical
Carla Queiros, Susana O. Ribeiro, Ana M. G. Silva, Andreia Leite
Summary: In this study, new rosamine-silica composites were prepared and their sensing ability towards different amines was evaluated. The composites showed significant sensitivity towards biogenic amines such as cadaverine and putrescine in solution, with a noticeable decrease in fluorescence intensity. The composite also exhibited fast response and reversibility in detecting putrescine vapors in powder form.
Article
Crystallography
Yuxiao Zhang, Xuntao Jiang, Xia Wu, Xiaoqiang Wang, Fang Huang, Kefei Li, Gaoyang Zheng, Shengzhou Lu, Yanxu Ma, Yuyu Zhou, Xiaoxi Yu
Summary: This study aimed to investigate the effects of nucleate agent sizes on lysozyme crystallization. The results showed that the size of silica nanoparticles had an impact on the morphological defects and quantity of lysozyme crystals. The interaction between silica nanoparticles and lysozyme was found to be mainly electrostatic, which increased with the size of the nanoparticles.
Article
Biotechnology & Applied Microbiology
Timothy A. Whitehead, Scott Banta, William E. Bentley, Michael J. Betenbaugh, Christina Chan, Douglas S. Clark, Corinne A. Hoesli, Michael C. Jewett, Beth Junker, Mattheos Koffas, Rashmi Kshirsagar, Amanda Lewis, Chien-Ting Li, Costas Maranas, E. Terry Papoutsakis, Kristala L. J. Prather, Steffen Schaffer, Laura Segatori, Ian Wheeldon
BIOTECHNOLOGY AND BIOENGINEERING
(2020)
Article
Biotechnology & Applied Microbiology
Yuta Inaba, Alan C. West, Scott Banta
BIOTECHNOLOGY AND BIOENGINEERING
(2020)
Article
Biochemistry & Molecular Biology
Virginia Jiang, Sagar D. Khare, Scott Banta
Summary: Cyc2 is a key protein in Acidithiobacillus ferrooxidans that mediates electron transfer between extracellular inorganic iron and intracellular central metabolism. A structural model of Cyc2 was proposed, highlighting a possible mechanism for the ferrous iron electron transfer chain, with identification of iron and heme docking sites. This computational model accounts for the membrane microenvironment and acidity in the extracellular matrix, and can be applied to model other TMBBs important for chemolithotrophic microbial growth.
Article
Biotechnology & Applied Microbiology
Yuta Inaba, Alan C. West, Scott Banta
Summary: The overexpression of glutathione in A. ferrooxidans enhances halotolerance and iron oxidation ability, allowing the cells to grow under inhibitory salt concentrations and pH conditions. Furthermore, the increase in glutathione levels leads to higher levels of intracellular reactive oxygen species, improving the cell's ability to tolerate oxidative stress in the presence of salt.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yuta Inaba, Timothy Kernan, Alan C. West, Scott Banta
Summary: The acidophilic chemolithoautotroph Acidithiobacillus ferrooxidans commonly exhibits diauxic population growth behavior where ferrous iron is oxidized before elemental sulfur despite the higher energy content of sulfur. Through the discovery of sulfur dispersion formulations, dispersed sulfur can be oxidized before ferrous iron, leading to rapid ferric iron reduction. This substrate utilization pattern is strongly influenced by cell loading, sulfur concentration, sulfur surface hydrophobicity, and culture pH.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Nadim Massad, Scott Banta
Summary: Protein engineering has been utilized to enhance the activities and stabilities of enzymes by introducing synthetic swing arms and utilizing modular assembly methods. Increasing NAD(H)-PEG swing arms has been shown to improve the performance of synthetic oxidoreductases, while also enabling the prediction of K-M values for new enzymes.
Article
Nanoscience & Nanotechnology
Heejung Jung, Yuta Inaba, Virginia Jiang, Alan C. West, Scott Banta
Summary: Metal processing using microorganisms has potential for reduced environmental impacts. This study demonstrated the enhanced metal binding capacity of Acidithiobacillus ferrooxidans through the fusion of polyhistidine tags to endogenous proteins. The cellular localization of the recombinant proteins correlated with their binding behavior, and the polyhistidine sequences showed selectivity for cobalt in mixed metal solutions.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Biochemical Research Methods
Walaa Abdallah, Xiao Hong, Scott Banta, Ian Wheeldon
Summary: Natural cascades often use spatial organization to introduce beneficial substrate channeling mechanisms, which has been widely adopted in engineered multienzyme cascades. Utilizing new molecular scaffolds now allows testing the effects of spatial organization on cascade kinetics, but these scaffolds can also change the microenvironment experienced by the assembled enzymes, complicating kinetic analysis.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Heejung Jung, Yuta Inaba, Scott Banta
Summary: Iron- and sulfur-oxidizing bacteria thrive in extremely acidic conditions and play important roles in biogeochemical cycles. They also have potential applications in industrial metal bioleaching and future biotechnology. However, their unique growth conditions make genetic techniques challenging. Researchers have successfully developed genetic tools and conducted genetic engineering on Acidithiobacillus ferrooxidans, a model organism with iron- and sulfur-oxidizing capabilities.
TRENDS IN BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Heejung Jung, Yuta Inaba, Jonathan T. Vardner, Alan C. West, Scott Banta
Summary: With the decrease in high-grade ore reserves, it is crucial to develop suitable metal extraction methods for processing low-grade materials. Bioleaching, an energy- and environmentally friendly process, is effective for processing copper and other sulfidic ores. Overexpression of licanantase protein in Acidithiobacillus ferrooxidans improves cell attachment and biofilm formation, enhancing the bioleaching efficiency of certain low-grade copper ores.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Emma Willett, Virginia Jiang, Ronald L. Koder, Scott Banta
Summary: NADK is the only enzyme capable of phosphorylating NAD(H) to NADP(H) and plays a crucial role in maintaining NAD(P)(H) homeostasis. The reverse activity of pigeon liver NADK was investigated, which can form ATP and NAD(+) from ADP and NADP(+). Four recombinant NADKs were characterized, showing that duck and cat NADKs have high activity in the reverse direction.
Article
Biotechnology & Applied Microbiology
Nadim Massad, Scott Banta
Summary: There is a growing interest in upgrading inexpensive and abundant C-1 feedstocks to higher carbon products. Linear carbon ligation routes are favored due to their simplicity and potential for high carbon efficiencies. This study provides a theoretical framework for comparing the performance of FLS enzymes, which is crucial for designing and implementing novel carbon ligation pathways.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Review
Biotechnology & Applied Microbiology
Ipek Simay Gokulu, Scott Banta
Summary: The functionalization of proteins with DNA through covalent bond formation enables various biotechnology advancements. DNA labeling of proteins allows for easy identification, organization, and control within DNA-protein networks. Bioconjugation reactions can target different amino acids within proteins, and further protein engineering methods can be used for conjugation reactions. The choice of technique depends on factors such as yield, selectivity, conjugation position, cost, and potential impact on protein function.
TRENDS IN BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Jonathan T. Vardner, Yuta Inaba, Heejung Jung, Raymond S. Farinato, D. R. Nagaraj, Scott Banta, Alan C. West
Summary: A hydrometallurgical process has been developed for the cost-effective production of copper during the global shift towards renewable energy technologies. Unlike conventional oxidative treatments, this process utilizes a reductive treatment of chalcopyrite using chromium(II) ions. This study demonstrates the successful reduction of chalcopyrite into copper(I) chloride at room temperature and pressure, with complete reaction within minutes. The reductive treatment is followed by leaching of the mineral products using iron(III) sulfate for complete copper extraction, and the chromium(II) ions can be regenerated through an electrolysis unit.
Article
Electrochemistry
Jonathan T. Vardner, Elifsu Gencer, Melissa Emerson, Raymond S. Farinato, D. R. Nagaraj, Scott Banta, Alan C. West
Summary: The demand for copper is projected to exceed supply in the coming decades due to the increased use of wind and solar technologies. This study introduces a potentially transformative hydrometallurgical process to effectively extract copper from chalcopyrite and ensure high copper production rates throughout the 21st century. By reacting chalcopyrite with vanadium (II) sulfate, copper can be rapidly, cleanly, and completely recovered.